Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran

Skin nodules of Onchocerca fasciata Railliet and Henry, 1910 (Spirurida, Onchocercidae) are a common finding in dromedary camels, though with a minimal clinical impact. There is little information about the morphology, molecular make-up and pathological impact of this parasite. Onchocerca fasciata n...

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Main Authors: Mirzaei Mohammad, Ghahvei Younes, Lefoulon Emilie, Lia Riccardo Paolo, Otranto Domenico, Martin Coralie, Sazmand Alireza
Format: Article
Language:English
Published: EDP Sciences 2018-01-01
Series:Parasite
Subjects:
Online Access:https://doi.org/10.1051/parasite/2018045
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author Mirzaei Mohammad
Ghahvei Younes
Lefoulon Emilie
Lia Riccardo Paolo
Otranto Domenico
Martin Coralie
Sazmand Alireza
author_facet Mirzaei Mohammad
Ghahvei Younes
Lefoulon Emilie
Lia Riccardo Paolo
Otranto Domenico
Martin Coralie
Sazmand Alireza
author_sort Mirzaei Mohammad
collection DOAJ
description Skin nodules of Onchocerca fasciata Railliet and Henry, 1910 (Spirurida, Onchocercidae) are a common finding in dromedary camels, though with a minimal clinical impact. There is little information about the morphology, molecular make-up and pathological impact of this parasite. Onchocerca fasciata nodules (1.3–2.1 cm in diameter and 509–841 mg in weight) were detected on the neck region in 31.5% of dromedary camels examined in Kerman province, southeastern Iran. Of 38 isolated nodules, only 23 (60.5%) contained viable worms. Measurement and morphological analyses were performed on isolated female worms by light microscopy. The identification of O. fasciata specimens was confirmed by sequence analysis of two mitochondrial genes (12S rDNA and cox1), which showed 0.4% divergence from available O. fasciata sequences. In addition, a phylogeny of filarial nematodes was constructed, based on these two mitochondrial genes and five nuclear genes (18S rDNA, 28S rDNA, MyoHC, rbp1, hsp70); this indicated that O. fasciata belongs to clade ONC3 of Onchocercidae, with representatives of the genera Onchocerca and Dirofilaria. Within the genus Onchocerca, O. fasciata is grouped with bovine parasitic species and the human parasitic Onchocerca volvulus, which suggests an impact of domestication on the radiation of the genus. Data provided here on the distribution and morphology of O. fasciata contribute to the molecular identification and phylogenetic position of the species.
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spelling doaj.art-d2af59771dfc4f5fa02e202da8199a442023-12-02T18:51:36ZengEDP SciencesParasite1776-10422018-01-01255010.1051/parasite/2018045parasite170153Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in IranMirzaei MohammadGhahvei YounesLefoulon EmilieLia Riccardo PaoloOtranto DomenicoMartin CoralieSazmand AlirezaSkin nodules of Onchocerca fasciata Railliet and Henry, 1910 (Spirurida, Onchocercidae) are a common finding in dromedary camels, though with a minimal clinical impact. There is little information about the morphology, molecular make-up and pathological impact of this parasite. Onchocerca fasciata nodules (1.3–2.1 cm in diameter and 509–841 mg in weight) were detected on the neck region in 31.5% of dromedary camels examined in Kerman province, southeastern Iran. Of 38 isolated nodules, only 23 (60.5%) contained viable worms. Measurement and morphological analyses were performed on isolated female worms by light microscopy. The identification of O. fasciata specimens was confirmed by sequence analysis of two mitochondrial genes (12S rDNA and cox1), which showed 0.4% divergence from available O. fasciata sequences. In addition, a phylogeny of filarial nematodes was constructed, based on these two mitochondrial genes and five nuclear genes (18S rDNA, 28S rDNA, MyoHC, rbp1, hsp70); this indicated that O. fasciata belongs to clade ONC3 of Onchocercidae, with representatives of the genera Onchocerca and Dirofilaria. Within the genus Onchocerca, O. fasciata is grouped with bovine parasitic species and the human parasitic Onchocerca volvulus, which suggests an impact of domestication on the radiation of the genus. Data provided here on the distribution and morphology of O. fasciata contribute to the molecular identification and phylogenetic position of the species.https://doi.org/10.1051/parasite/2018045Onchocerca fasciataVector-borne diseaseLight microscopyPhylogenydromedary
spellingShingle Mirzaei Mohammad
Ghahvei Younes
Lefoulon Emilie
Lia Riccardo Paolo
Otranto Domenico
Martin Coralie
Sazmand Alireza
Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran
Parasite
Onchocerca fasciata
Vector-borne disease
Light microscopy
Phylogeny
dromedary
title Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran
title_full Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran
title_fullStr Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran
title_full_unstemmed Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran
title_short Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran
title_sort morphological and molecular characterization of onchocerca fasciata nematoda onchocercidae from dromedary camels camelus dromedarius in iran
topic Onchocerca fasciata
Vector-borne disease
Light microscopy
Phylogeny
dromedary
url https://doi.org/10.1051/parasite/2018045
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