Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran
Skin nodules of Onchocerca fasciata Railliet and Henry, 1910 (Spirurida, Onchocercidae) are a common finding in dromedary camels, though with a minimal clinical impact. There is little information about the morphology, molecular make-up and pathological impact of this parasite. Onchocerca fasciata n...
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EDP Sciences
2018-01-01
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Online Access: | https://doi.org/10.1051/parasite/2018045 |
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author | Mirzaei Mohammad Ghahvei Younes Lefoulon Emilie Lia Riccardo Paolo Otranto Domenico Martin Coralie Sazmand Alireza |
author_facet | Mirzaei Mohammad Ghahvei Younes Lefoulon Emilie Lia Riccardo Paolo Otranto Domenico Martin Coralie Sazmand Alireza |
author_sort | Mirzaei Mohammad |
collection | DOAJ |
description | Skin nodules of Onchocerca fasciata Railliet and Henry, 1910 (Spirurida, Onchocercidae) are a common finding in dromedary camels, though with a minimal clinical impact. There is little information about the morphology, molecular make-up and pathological impact of this parasite. Onchocerca fasciata nodules (1.3–2.1 cm in diameter and 509–841 mg in weight) were detected on the neck region in 31.5% of dromedary camels examined in Kerman province, southeastern Iran. Of 38 isolated nodules, only 23 (60.5%) contained viable worms. Measurement and morphological analyses were performed on isolated female worms by light microscopy. The identification of O. fasciata specimens was confirmed by sequence analysis of two mitochondrial genes (12S rDNA and cox1), which showed 0.4% divergence from available O. fasciata sequences. In addition, a phylogeny of filarial nematodes was constructed, based on these two mitochondrial genes and five nuclear genes (18S rDNA, 28S rDNA, MyoHC, rbp1, hsp70); this indicated that O. fasciata belongs to clade ONC3 of Onchocercidae, with representatives of the genera Onchocerca and Dirofilaria. Within the genus Onchocerca, O. fasciata is grouped with bovine parasitic species and the human parasitic Onchocerca volvulus, which suggests an impact of domestication on the radiation of the genus. Data provided here on the distribution and morphology of O. fasciata contribute to the molecular identification and phylogenetic position of the species. |
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language | English |
last_indexed | 2024-03-09T08:34:26Z |
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spelling | doaj.art-d2af59771dfc4f5fa02e202da8199a442023-12-02T18:51:36ZengEDP SciencesParasite1776-10422018-01-01255010.1051/parasite/2018045parasite170153Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in IranMirzaei MohammadGhahvei YounesLefoulon EmilieLia Riccardo PaoloOtranto DomenicoMartin CoralieSazmand AlirezaSkin nodules of Onchocerca fasciata Railliet and Henry, 1910 (Spirurida, Onchocercidae) are a common finding in dromedary camels, though with a minimal clinical impact. There is little information about the morphology, molecular make-up and pathological impact of this parasite. Onchocerca fasciata nodules (1.3–2.1 cm in diameter and 509–841 mg in weight) were detected on the neck region in 31.5% of dromedary camels examined in Kerman province, southeastern Iran. Of 38 isolated nodules, only 23 (60.5%) contained viable worms. Measurement and morphological analyses were performed on isolated female worms by light microscopy. The identification of O. fasciata specimens was confirmed by sequence analysis of two mitochondrial genes (12S rDNA and cox1), which showed 0.4% divergence from available O. fasciata sequences. In addition, a phylogeny of filarial nematodes was constructed, based on these two mitochondrial genes and five nuclear genes (18S rDNA, 28S rDNA, MyoHC, rbp1, hsp70); this indicated that O. fasciata belongs to clade ONC3 of Onchocercidae, with representatives of the genera Onchocerca and Dirofilaria. Within the genus Onchocerca, O. fasciata is grouped with bovine parasitic species and the human parasitic Onchocerca volvulus, which suggests an impact of domestication on the radiation of the genus. Data provided here on the distribution and morphology of O. fasciata contribute to the molecular identification and phylogenetic position of the species.https://doi.org/10.1051/parasite/2018045Onchocerca fasciataVector-borne diseaseLight microscopyPhylogenydromedary |
spellingShingle | Mirzaei Mohammad Ghahvei Younes Lefoulon Emilie Lia Riccardo Paolo Otranto Domenico Martin Coralie Sazmand Alireza Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran Parasite Onchocerca fasciata Vector-borne disease Light microscopy Phylogeny dromedary |
title | Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran |
title_full | Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran |
title_fullStr | Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran |
title_full_unstemmed | Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran |
title_short | Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran |
title_sort | morphological and molecular characterization of onchocerca fasciata nematoda onchocercidae from dromedary camels camelus dromedarius in iran |
topic | Onchocerca fasciata Vector-borne disease Light microscopy Phylogeny dromedary |
url | https://doi.org/10.1051/parasite/2018045 |
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