Assessment of ALK Rearrangement in Non-small Cell Lung Cancer: Using Enhancing Immunohistochemical Way and Fluorescence in situ Hybridization
Background and objective Besides epidermal growth factor receptor (EGFR) mutation, the non-small cell lung cancer (NSCLC) of anaplastic lymphoma kinase (ALK) rearrangement becomes another important clinical subtype. A specific and high-sensitive and economical detection way is convenience for identi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2015-02-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2015.02.04 |
| _version_ | 1818684770810331136 |
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| author | Hui MENG Xianzheng GAO Lan ZHANG Fang LIU Wencai LI |
| author_facet | Hui MENG Xianzheng GAO Lan ZHANG Fang LIU Wencai LI |
| author_sort | Hui MENG |
| collection | DOAJ |
| description | Background and objective Besides epidermal growth factor receptor (EGFR) mutation, the non-small cell lung cancer (NSCLC) of anaplastic lymphoma kinase (ALK) rearrangement becomes another important clinical subtype. A specific and high-sensitive and economical detection way is convenience for identification of ALK positive NSCLC quickly and accurately. So the objective of our research is to detect ALK rearrangement in 172 cases of NSCLC by using enhancing immunohistochemical way (ventana-IHC, V-IHC). Methods ALK rearrangement in 172 NSCLC samples was detected by using V-IHC, and positive staining cases were further verified by fluorescence in situ hybridization (FISH). Results Among 172 NSCLC cases, there were 12 positive staining. The positive results were confirmed by FISH and 11 cases were FISH positive. The overall concordance between V-IHC and FISH is 91.7% (11/12). Conclusion The V-IHC method is a reliable method for ALK arrangement and could be used in clinical screen and diagnosis. |
| first_indexed | 2024-12-17T10:55:55Z |
| format | Article |
| id | doaj.art-d2b042dc7bd7450985d091bc76e450d8 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 |
| language | zho |
| last_indexed | 2024-12-17T10:55:55Z |
| publishDate | 2015-02-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-d2b042dc7bd7450985d091bc76e450d82022-12-21T21:51:49ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34192015-02-01182757910.3779/j.issn.1009-3419.2015.02.04Assessment of ALK Rearrangement in Non-small Cell Lung Cancer: Using Enhancing Immunohistochemical Way and Fluorescence in situ HybridizationHui MENG0Xianzheng GAO1Lan ZHANG2Fang LIU3Wencai LI4Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaPfizer Oncology Medical Affairs, Chengdu 610041, ChinaDepartment of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaBackground and objective Besides epidermal growth factor receptor (EGFR) mutation, the non-small cell lung cancer (NSCLC) of anaplastic lymphoma kinase (ALK) rearrangement becomes another important clinical subtype. A specific and high-sensitive and economical detection way is convenience for identification of ALK positive NSCLC quickly and accurately. So the objective of our research is to detect ALK rearrangement in 172 cases of NSCLC by using enhancing immunohistochemical way (ventana-IHC, V-IHC). Methods ALK rearrangement in 172 NSCLC samples was detected by using V-IHC, and positive staining cases were further verified by fluorescence in situ hybridization (FISH). Results Among 172 NSCLC cases, there were 12 positive staining. The positive results were confirmed by FISH and 11 cases were FISH positive. The overall concordance between V-IHC and FISH is 91.7% (11/12). Conclusion The V-IHC method is a reliable method for ALK arrangement and could be used in clinical screen and diagnosis.http://dx.doi.org/10.3779/j.issn.1009-3419.2015.02.04Lung neoplasmsALKVentana-IHCFISH |
| spellingShingle | Hui MENG Xianzheng GAO Lan ZHANG Fang LIU Wencai LI Assessment of ALK Rearrangement in Non-small Cell Lung Cancer: Using Enhancing Immunohistochemical Way and Fluorescence in situ Hybridization Chinese Journal of Lung Cancer Lung neoplasms ALK Ventana-IHC FISH |
| title | Assessment of ALK Rearrangement in Non-small Cell Lung Cancer: Using Enhancing Immunohistochemical Way and Fluorescence in situ Hybridization |
| title_full | Assessment of ALK Rearrangement in Non-small Cell Lung Cancer: Using Enhancing Immunohistochemical Way and Fluorescence in situ Hybridization |
| title_fullStr | Assessment of ALK Rearrangement in Non-small Cell Lung Cancer: Using Enhancing Immunohistochemical Way and Fluorescence in situ Hybridization |
| title_full_unstemmed | Assessment of ALK Rearrangement in Non-small Cell Lung Cancer: Using Enhancing Immunohistochemical Way and Fluorescence in situ Hybridization |
| title_short | Assessment of ALK Rearrangement in Non-small Cell Lung Cancer: Using Enhancing Immunohistochemical Way and Fluorescence in situ Hybridization |
| title_sort | assessment of alk rearrangement in non small cell lung cancer using enhancing immunohistochemical way and fluorescence in situ hybridization |
| topic | Lung neoplasms ALK Ventana-IHC FISH |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2015.02.04 |
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