The effect of enterohemorrhagic E. coli infection on the cell mechanics of host cells.
Enterohaemorrhagic E. coli (EHEC) is a type of human pathogenic bacteria. The main virulence characteristics of EHEC include the formation of attaching and effacing lesions (A/E lesions) and the production of one or more Shiga-like toxins, which may induce human uremic complications. When EHEC infec...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4219835?pdf=render |
| _version_ | 1818025409193705472 |
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| author | Yin-Quan Chen Pin-Tzu Su Yu-Hsuan Chen Ming-Tzo Wei Chien-Hsiu Huang Kathryn Osterday Juan C del Álamo Wan-Jr Syu Arthur Chiou |
| author_facet | Yin-Quan Chen Pin-Tzu Su Yu-Hsuan Chen Ming-Tzo Wei Chien-Hsiu Huang Kathryn Osterday Juan C del Álamo Wan-Jr Syu Arthur Chiou |
| author_sort | Yin-Quan Chen |
| collection | DOAJ |
| description | Enterohaemorrhagic E. coli (EHEC) is a type of human pathogenic bacteria. The main virulence characteristics of EHEC include the formation of attaching and effacing lesions (A/E lesions) and the production of one or more Shiga-like toxins, which may induce human uremic complications. When EHEC infects host cells, it releases translocated intimin receptor (Tir) and effector proteins inside the host cells, inducing the rearrangement and accumulation of the F-actin cytoskeleton, a phenotype leading to the formation of pedestals in the apical cell surface, and the growth of stress fibers at the base of the cells. To examine the effect of EHEC infection on cell mechanics, we carried out a series of experiments to examine HeLa cells with and without EHEC infection to quantify the changes in (1) focal adhesion area, visualized by anti-vinculin staining; (2) the distribution and orientation of stress fibers; and (3) the intracellular viscoelasticity, via directional video particle tracking microrheology. Our results indicated that in EHEC-infected HeLa cells, the focal adhesion area increased and the actin stress fibers became thicker and more aligned. The cytoskeletal reorganization induced by EHEC infection mediated a dramatic increase in the cytoplasmic elastic shear modulus of the infected cells, and a transition in the viscoelastic behavior of the cells from viscous-like to elastic-like. These changes in mechanobiological characteristics might modulate the attachments between EHEC and the host cell to withstand exfoliation, and between the host cell and the extracellular matrix, and might also alter epithelial integrity. |
| first_indexed | 2024-12-10T04:15:39Z |
| format | Article |
| id | doaj.art-d2b8345f6fa6432482239be00585178d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T04:15:39Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d2b8345f6fa6432482239be00585178d2022-12-22T02:02:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11213710.1371/journal.pone.0112137The effect of enterohemorrhagic E. coli infection on the cell mechanics of host cells.Yin-Quan ChenPin-Tzu SuYu-Hsuan ChenMing-Tzo WeiChien-Hsiu HuangKathryn OsterdayJuan C del ÁlamoWan-Jr SyuArthur ChiouEnterohaemorrhagic E. coli (EHEC) is a type of human pathogenic bacteria. The main virulence characteristics of EHEC include the formation of attaching and effacing lesions (A/E lesions) and the production of one or more Shiga-like toxins, which may induce human uremic complications. When EHEC infects host cells, it releases translocated intimin receptor (Tir) and effector proteins inside the host cells, inducing the rearrangement and accumulation of the F-actin cytoskeleton, a phenotype leading to the formation of pedestals in the apical cell surface, and the growth of stress fibers at the base of the cells. To examine the effect of EHEC infection on cell mechanics, we carried out a series of experiments to examine HeLa cells with and without EHEC infection to quantify the changes in (1) focal adhesion area, visualized by anti-vinculin staining; (2) the distribution and orientation of stress fibers; and (3) the intracellular viscoelasticity, via directional video particle tracking microrheology. Our results indicated that in EHEC-infected HeLa cells, the focal adhesion area increased and the actin stress fibers became thicker and more aligned. The cytoskeletal reorganization induced by EHEC infection mediated a dramatic increase in the cytoplasmic elastic shear modulus of the infected cells, and a transition in the viscoelastic behavior of the cells from viscous-like to elastic-like. These changes in mechanobiological characteristics might modulate the attachments between EHEC and the host cell to withstand exfoliation, and between the host cell and the extracellular matrix, and might also alter epithelial integrity.http://europepmc.org/articles/PMC4219835?pdf=render |
| spellingShingle | Yin-Quan Chen Pin-Tzu Su Yu-Hsuan Chen Ming-Tzo Wei Chien-Hsiu Huang Kathryn Osterday Juan C del Álamo Wan-Jr Syu Arthur Chiou The effect of enterohemorrhagic E. coli infection on the cell mechanics of host cells. PLoS ONE |
| title | The effect of enterohemorrhagic E. coli infection on the cell mechanics of host cells. |
| title_full | The effect of enterohemorrhagic E. coli infection on the cell mechanics of host cells. |
| title_fullStr | The effect of enterohemorrhagic E. coli infection on the cell mechanics of host cells. |
| title_full_unstemmed | The effect of enterohemorrhagic E. coli infection on the cell mechanics of host cells. |
| title_short | The effect of enterohemorrhagic E. coli infection on the cell mechanics of host cells. |
| title_sort | effect of enterohemorrhagic e coli infection on the cell mechanics of host cells |
| url | http://europepmc.org/articles/PMC4219835?pdf=render |
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