Early Neonatal Meconium Does Not Have a Demonstrable Microbiota Determined through Use of Robust Negative Controls with cpn60-Based Microbiome Profiling

Early Neonatal Meconium Does Not Have a Demonstrable Microbiota Determined through Use of Robust Negative Controls with cpn60-Based Microbiome Profiling

ABSTRACT Detection of bacterial DNA within meconium is often cited as evidence supporting in utero colonization. However, many studies fail to adequately control for contamination. We aimed to define the microbial content of meconium under properly controlled conditions. DNA was extracted from 141 m...

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Bibliographic Details
Main Authors: Scott J. Dos Santos, Zahra Pakzad, Chelsea N. Elwood, Arianne Y. K. Albert, Soren Gantt, Amee R. Manges, Tim J. Dumonceaux, Evelyn J. Maan, Janet E. Hill, Deborah M. Money
Format: Article
Language:English
Published: American Society for Microbiology 2021-10-01
Series:Microbiology Spectrum
Subjects:
contamination
cpn60
low biomass
meconium
microbiome
negative controls
Online Access:https://journals.asm.org/doi/10.1128/Spectrum.00067-21

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