The Involvement of Energy Metabolism and Lipid Peroxidation in Lignin Accumulation of Postharvest Pumelos
Lignification is especially prominent in postharvest pumelo fruit, which greatly impairs their attractiveness and commercial value. This study investigated the energy metabolism and lipid peroxidation and their relationship with accumulated lignin content in juice sacs of “Hongroumiyou” (HR) during...
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MDPI AG
2020-09-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/10/10/269 |
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| author | Huiling Yan Junjia Chen Juan Liu |
| author_facet | Huiling Yan Junjia Chen Juan Liu |
| author_sort | Huiling Yan |
| collection | DOAJ |
| description | Lignification is especially prominent in postharvest pumelo fruit, which greatly impairs their attractiveness and commercial value. This study investigated the energy metabolism and lipid peroxidation and their relationship with accumulated lignin content in juice sacs of “Hongroumiyou” (HR) during 90 d of storage at 25 °C. The results indicated that, the alterations of energy metabolism in juice of sacs of postharvest pumelos was featured by a continuous decline in energy charge and ATP/ADP; an increase in succinic dehydrogenase (SDH) activity before 30 d and increases in activities of cytochrome c oxidase (CCO) and F<sub>0</sub>F<sub>1</sub>-ATPase before 60 d; but declines in activities of Ca<sup>2+</sup>-ATPase and H<sup>+</sup>-ATPase. Additionally, enhanced contents of H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>−</sup>, and –OH scavenging rate; increased malondialdehyde (MDA) content; and transformation of unsaturated fatty acids (USFA) to saturated fatty acids (USFA) and reduced USFA/SFA (U/S) could result in lipid peroxidation and membrane integrity loss. Moreover, correlation analysis showed that lignin accumulation was in close relation to energy metabolism and lipid peroxidation in juice sacs of postharvest pumelos. These results gave evident credence for the involvement of energy metabolism and lipid peroxidation in the lignin accumulation of HR pumelo fruit during postharvest storage. |
| first_indexed | 2024-03-10T15:57:14Z |
| format | Article |
| id | doaj.art-d2ba7ad72b0d4cdc9e00bef1b1798483 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-10T15:57:14Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-d2ba7ad72b0d4cdc9e00bef1b17984832023-11-20T15:36:44ZengMDPI AGMembranes2077-03752020-09-01101026910.3390/membranes10100269The Involvement of Energy Metabolism and Lipid Peroxidation in Lignin Accumulation of Postharvest PumelosHuiling Yan0Junjia Chen1Juan Liu2Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaGuangdong Engineering Lab of High Value Utilization of Biomass, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaGuangdong Engineering Lab of High Value Utilization of Biomass, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaLignification is especially prominent in postharvest pumelo fruit, which greatly impairs their attractiveness and commercial value. This study investigated the energy metabolism and lipid peroxidation and their relationship with accumulated lignin content in juice sacs of “Hongroumiyou” (HR) during 90 d of storage at 25 °C. The results indicated that, the alterations of energy metabolism in juice of sacs of postharvest pumelos was featured by a continuous decline in energy charge and ATP/ADP; an increase in succinic dehydrogenase (SDH) activity before 30 d and increases in activities of cytochrome c oxidase (CCO) and F<sub>0</sub>F<sub>1</sub>-ATPase before 60 d; but declines in activities of Ca<sup>2+</sup>-ATPase and H<sup>+</sup>-ATPase. Additionally, enhanced contents of H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>−</sup>, and –OH scavenging rate; increased malondialdehyde (MDA) content; and transformation of unsaturated fatty acids (USFA) to saturated fatty acids (USFA) and reduced USFA/SFA (U/S) could result in lipid peroxidation and membrane integrity loss. Moreover, correlation analysis showed that lignin accumulation was in close relation to energy metabolism and lipid peroxidation in juice sacs of postharvest pumelos. These results gave evident credence for the involvement of energy metabolism and lipid peroxidation in the lignin accumulation of HR pumelo fruit during postharvest storage.https://www.mdpi.com/2077-0375/10/10/269pumeloligninenergyROSlipid peroxidationmembrane integrity |
| spellingShingle | Huiling Yan Junjia Chen Juan Liu The Involvement of Energy Metabolism and Lipid Peroxidation in Lignin Accumulation of Postharvest Pumelos Membranes pumelo lignin energy ROS lipid peroxidation membrane integrity |
| title | The Involvement of Energy Metabolism and Lipid Peroxidation in Lignin Accumulation of Postharvest Pumelos |
| title_full | The Involvement of Energy Metabolism and Lipid Peroxidation in Lignin Accumulation of Postharvest Pumelos |
| title_fullStr | The Involvement of Energy Metabolism and Lipid Peroxidation in Lignin Accumulation of Postharvest Pumelos |
| title_full_unstemmed | The Involvement of Energy Metabolism and Lipid Peroxidation in Lignin Accumulation of Postharvest Pumelos |
| title_short | The Involvement of Energy Metabolism and Lipid Peroxidation in Lignin Accumulation of Postharvest Pumelos |
| title_sort | involvement of energy metabolism and lipid peroxidation in lignin accumulation of postharvest pumelos |
| topic | pumelo lignin energy ROS lipid peroxidation membrane integrity |
| url | https://www.mdpi.com/2077-0375/10/10/269 |
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