Detection of <em>Coxiella burnetii</em> in milk of domestic cattle in Ecuador
<p>Ruminants are the main reservoir of <em>Coxiella burnetii</em>, an obligate intracellular Gram negative pathogen. This microorganism is the causative agent of Q fever, a a zoonosis transmited to humans by contacting contaminated fluids from infected animals, inhaled aerosols or...
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| Format: | Article |
| Language: | English |
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Universidad San Francisco de Quito USFQ
2013-04-01
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| Series: | ACI Avances en Ciencias e Ingenierías |
| Subjects: | |
| Online Access: | http://revistas.usfq.edu.ec/index.php/avances/article/view/115 |
| _version_ | 1797285294389592064 |
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| author | María Isabel Rojas Verónica Barragán Gabriel A. Trueba P. Heidie M. Hornstra O'Neill Talima Pearson Paul Keim |
| author_facet | María Isabel Rojas Verónica Barragán Gabriel A. Trueba P. Heidie M. Hornstra O'Neill Talima Pearson Paul Keim |
| author_sort | María Isabel Rojas |
| collection | DOAJ |
| description | <p>Ruminants are the main reservoir of <em>Coxiella burnetii</em>, an obligate intracellular Gram negative pathogen. This microorganism is the causative agent of Q fever, a a zoonosis transmited to humans by contacting contaminated fluids from infected animals, inhaled aerosols or consuming milk from infected animals. We used Real-Time PCR assay with a Taq-Man fluorescent probe to detect the pathogen in samples of bovine milk collected from random locations in nine Ecuadorian provinces. The assay targeted a 61-63bp repetitive sequence within the IS1111 transposon in <em>C. burnetii</em>. The assay found positive samples in two provinces located close to the Colombian and Peruvian borders and in Chimborazo (Ecuadorian central region). This study is the first in Ecuador to detect <em>Coxiella burnetii</em> using a molecular method of high sensitivity.</p> |
| first_indexed | 2024-03-07T18:00:05Z |
| format | Article |
| id | doaj.art-d2bad1de084846a98ef0477f9b71b265 |
| institution | Directory Open Access Journal |
| issn | 1390-5384 2528-7788 |
| language | English |
| last_indexed | 2024-03-07T18:00:05Z |
| publishDate | 2013-04-01 |
| publisher | Universidad San Francisco de Quito USFQ |
| record_format | Article |
| series | ACI Avances en Ciencias e Ingenierías |
| spelling | doaj.art-d2bad1de084846a98ef0477f9b71b2652024-03-02T11:03:27ZengUniversidad San Francisco de Quito USFQACI Avances en Ciencias e Ingenierías1390-53842528-77882013-04-015110.18272/aci.v5i1.115115Detection of <em>Coxiella burnetii</em> in milk of domestic cattle in EcuadorMaría Isabel Rojas0Verónica Barragán1Gabriel A. Trueba P.2Heidie M. Hornstra O'Neill3Talima Pearson4Paul Keim5Universidad San Francisco de QuitoUniversidad San Francisco de QuitoUniversidad San Francisco de QuitoNorthern Arizona UniversityNorthern Arizona UniversityNorthern Arizona University<p>Ruminants are the main reservoir of <em>Coxiella burnetii</em>, an obligate intracellular Gram negative pathogen. This microorganism is the causative agent of Q fever, a a zoonosis transmited to humans by contacting contaminated fluids from infected animals, inhaled aerosols or consuming milk from infected animals. We used Real-Time PCR assay with a Taq-Man fluorescent probe to detect the pathogen in samples of bovine milk collected from random locations in nine Ecuadorian provinces. The assay targeted a 61-63bp repetitive sequence within the IS1111 transposon in <em>C. burnetii</em>. The assay found positive samples in two provinces located close to the Colombian and Peruvian borders and in Chimborazo (Ecuadorian central region). This study is the first in Ecuador to detect <em>Coxiella burnetii</em> using a molecular method of high sensitivity.</p>http://revistas.usfq.edu.ec/index.php/avances/article/view/115Fiebre QCoxiella burnetiiPCR en tiempo real |
| spellingShingle | María Isabel Rojas Verónica Barragán Gabriel A. Trueba P. Heidie M. Hornstra O'Neill Talima Pearson Paul Keim Detection of <em>Coxiella burnetii</em> in milk of domestic cattle in Ecuador ACI Avances en Ciencias e Ingenierías Fiebre Q Coxiella burnetii PCR en tiempo real |
| title | Detection of <em>Coxiella burnetii</em> in milk of domestic cattle in Ecuador |
| title_full | Detection of <em>Coxiella burnetii</em> in milk of domestic cattle in Ecuador |
| title_fullStr | Detection of <em>Coxiella burnetii</em> in milk of domestic cattle in Ecuador |
| title_full_unstemmed | Detection of <em>Coxiella burnetii</em> in milk of domestic cattle in Ecuador |
| title_short | Detection of <em>Coxiella burnetii</em> in milk of domestic cattle in Ecuador |
| title_sort | detection of em coxiella burnetii em in milk of domestic cattle in ecuador |
| topic | Fiebre Q Coxiella burnetii PCR en tiempo real |
| url | http://revistas.usfq.edu.ec/index.php/avances/article/view/115 |
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