Vaccination against Streptococcus pneumoniae using truncated derivatives of polyhistidine triad protein D.
Polyhistidine triad protein D (PhtD) has been described as a promising vaccine candidate for use against Streptococcus pneumoniae, but there has been a lack of examination of its structure and of which region(s) of the protein are targeted by protective immune responses. In this study, we purified r...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3814962?pdf=render |
| _version_ | 1811232875227381760 |
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| author | Charles D Plumptre Abiodun D Ogunniyi James C Paton |
| author_facet | Charles D Plumptre Abiodun D Ogunniyi James C Paton |
| author_sort | Charles D Plumptre |
| collection | DOAJ |
| description | Polyhistidine triad protein D (PhtD) has been described as a promising vaccine candidate for use against Streptococcus pneumoniae, but there has been a lack of examination of its structure and of which region(s) of the protein are targeted by protective immune responses. In this study, we purified recombinant truncated derivatives of PhtD and examined their secondary structural composition, as well as their capacity to bind antibodies from polyclonal murine serum generated against the full length protein. This allowed the identification of a particularly immunogenic fragment of PhtD, which was also purified and characterised. The truncated derivatives were tested as vaccine antigens in mouse models of pneumococcal sepsis and colonisation, using alum and E. coli heat labile toxin B subunit respectively as adjuvants. These experiments revealed that whilst the immunogenic region identified may be a promising candidate to protect against sepsis, the full length PhtD was ineffective at conferring significant protective immunity. These results are significant for the potential for PhtD to be used in novel vaccines, which are currently being tested in clinical trials. |
| first_indexed | 2024-04-12T11:11:44Z |
| format | Article |
| id | doaj.art-d2bbac1a3afe46a19e100a66fbeb5ab1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T11:11:44Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d2bbac1a3afe46a19e100a66fbeb5ab12022-12-22T03:35:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7891610.1371/journal.pone.0078916Vaccination against Streptococcus pneumoniae using truncated derivatives of polyhistidine triad protein D.Charles D PlumptreAbiodun D OgunniyiJames C PatonPolyhistidine triad protein D (PhtD) has been described as a promising vaccine candidate for use against Streptococcus pneumoniae, but there has been a lack of examination of its structure and of which region(s) of the protein are targeted by protective immune responses. In this study, we purified recombinant truncated derivatives of PhtD and examined their secondary structural composition, as well as their capacity to bind antibodies from polyclonal murine serum generated against the full length protein. This allowed the identification of a particularly immunogenic fragment of PhtD, which was also purified and characterised. The truncated derivatives were tested as vaccine antigens in mouse models of pneumococcal sepsis and colonisation, using alum and E. coli heat labile toxin B subunit respectively as adjuvants. These experiments revealed that whilst the immunogenic region identified may be a promising candidate to protect against sepsis, the full length PhtD was ineffective at conferring significant protective immunity. These results are significant for the potential for PhtD to be used in novel vaccines, which are currently being tested in clinical trials.http://europepmc.org/articles/PMC3814962?pdf=render |
| spellingShingle | Charles D Plumptre Abiodun D Ogunniyi James C Paton Vaccination against Streptococcus pneumoniae using truncated derivatives of polyhistidine triad protein D. PLoS ONE |
| title | Vaccination against Streptococcus pneumoniae using truncated derivatives of polyhistidine triad protein D. |
| title_full | Vaccination against Streptococcus pneumoniae using truncated derivatives of polyhistidine triad protein D. |
| title_fullStr | Vaccination against Streptococcus pneumoniae using truncated derivatives of polyhistidine triad protein D. |
| title_full_unstemmed | Vaccination against Streptococcus pneumoniae using truncated derivatives of polyhistidine triad protein D. |
| title_short | Vaccination against Streptococcus pneumoniae using truncated derivatives of polyhistidine triad protein D. |
| title_sort | vaccination against streptococcus pneumoniae using truncated derivatives of polyhistidine triad protein d |
| url | http://europepmc.org/articles/PMC3814962?pdf=render |
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