Validation of a high-throughput and robust technique: BACs-on-beads assay (KaryoLite BoBs) for pre-implantation aneuploidy screening
Objective: This study aims to validate the BACs-on-Beads (BoB) technology as a robust and high throughput method for pre-implantation genetic screening (PGS) for aneuploidy. Material and methods: The performances with respect to the sensitivity, specificity, success rate and detection rate of this t...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-08-01
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| Series: | Taiwanese Journal of Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1028455917301493 |
| _version_ | 1818023472891166720 |
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| author | Grace Wing Shan Kong Yanlin Ma Jian Ou Yvonne Ka Yin Kwok Wei Wang Queenie Sum Yee Yeung Cherry Kit Man Wong Qi Li Wen Xu Weiying Lu Hong Li Tin Chiu Li Kwong Wai Choy |
| author_facet | Grace Wing Shan Kong Yanlin Ma Jian Ou Yvonne Ka Yin Kwok Wei Wang Queenie Sum Yee Yeung Cherry Kit Man Wong Qi Li Wen Xu Weiying Lu Hong Li Tin Chiu Li Kwong Wai Choy |
| author_sort | Grace Wing Shan Kong |
| collection | DOAJ |
| description | Objective: This study aims to validate the BACs-on-Beads (BoB) technology as a robust and high throughput method for pre-implantation genetic screening (PGS) for aneuploidy.
Material and methods: The performances with respect to the sensitivity, specificity, success rate and detection rate of this technique from new BoBs technology and traditional array chromosomal genomic hybridization (aCGH) were compared. And the use of BoBs as a screening tool for euploid embryos in PGS was evaluated.
Result: In the first part of validation study, there were total 75 embryos completed PGS by both BoBs and aCGH. The success rate of PGS was 97.4%, and the results showed 100% concordance between BoBs and aCGH for aneuploidy. In the second part, a total 219 embryos were involved. The success rate of PGS by BoBs was 100%. BoBs identified 28% (62/219) euploidy which were further confirmed to be euploidy by aCGH.
Conclusion: This new strategic approach using BoBs as a first tier PGS screening tool and aCGH as a confirmatory tool can increase the throughput of PGS with a reduced cost and time to meet the demand in high volume units. |
| first_indexed | 2024-12-10T03:44:52Z |
| format | Article |
| id | doaj.art-d2bdba40b45f470fa40347a89274e785 |
| institution | Directory Open Access Journal |
| issn | 1028-4559 |
| language | English |
| last_indexed | 2024-12-10T03:44:52Z |
| publishDate | 2017-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Taiwanese Journal of Obstetrics & Gynecology |
| spelling | doaj.art-d2bdba40b45f470fa40347a89274e7852022-12-22T02:03:27ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592017-08-0156451452010.1016/j.tjog.2017.01.011Validation of a high-throughput and robust technique: BACs-on-beads assay (KaryoLite BoBs) for pre-implantation aneuploidy screeningGrace Wing Shan Kong0Yanlin Ma1Jian Ou2Yvonne Ka Yin Kwok3Wei Wang4Queenie Sum Yee Yeung5Cherry Kit Man Wong6Qi Li7Wen Xu8Weiying Lu9Hong Li10Tin Chiu Li11Kwong Wai Choy12Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, ChinaAssisted Reproductive Technology Center, Affiliated Hospital, Hainan Medical University, Haikou, 570102, PR ChinaCenter of Reproduction and Genetics Suzhou Municipal Hospital, Jiangsu Suzhou 215002, PR ChinaDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, ChinaCenter of Reproduction and Genetics Suzhou Municipal Hospital, Jiangsu Suzhou 215002, PR ChinaDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, ChinaDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, ChinaAssisted Reproductive Technology Center, Affiliated Hospital, Hainan Medical University, Haikou, 570102, PR ChinaAssisted Reproductive Technology Center, Affiliated Hospital, Hainan Medical University, Haikou, 570102, PR ChinaAssisted Reproductive Technology Center, Affiliated Hospital, Hainan Medical University, Haikou, 570102, PR ChinaCenter of Reproduction and Genetics Suzhou Municipal Hospital, Jiangsu Suzhou 215002, PR ChinaDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, ChinaDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, ChinaObjective: This study aims to validate the BACs-on-Beads (BoB) technology as a robust and high throughput method for pre-implantation genetic screening (PGS) for aneuploidy. Material and methods: The performances with respect to the sensitivity, specificity, success rate and detection rate of this technique from new BoBs technology and traditional array chromosomal genomic hybridization (aCGH) were compared. And the use of BoBs as a screening tool for euploid embryos in PGS was evaluated. Result: In the first part of validation study, there were total 75 embryos completed PGS by both BoBs and aCGH. The success rate of PGS was 97.4%, and the results showed 100% concordance between BoBs and aCGH for aneuploidy. In the second part, a total 219 embryos were involved. The success rate of PGS by BoBs was 100%. BoBs identified 28% (62/219) euploidy which were further confirmed to be euploidy by aCGH. Conclusion: This new strategic approach using BoBs as a first tier PGS screening tool and aCGH as a confirmatory tool can increase the throughput of PGS with a reduced cost and time to meet the demand in high volume units.http://www.sciencedirect.com/science/article/pii/S1028455917301493BoBKaryoliteaCGHPGSAneuploidy |
| spellingShingle | Grace Wing Shan Kong Yanlin Ma Jian Ou Yvonne Ka Yin Kwok Wei Wang Queenie Sum Yee Yeung Cherry Kit Man Wong Qi Li Wen Xu Weiying Lu Hong Li Tin Chiu Li Kwong Wai Choy Validation of a high-throughput and robust technique: BACs-on-beads assay (KaryoLite BoBs) for pre-implantation aneuploidy screening Taiwanese Journal of Obstetrics & Gynecology BoB Karyolite aCGH PGS Aneuploidy |
| title | Validation of a high-throughput and robust technique: BACs-on-beads assay (KaryoLite BoBs) for pre-implantation aneuploidy screening |
| title_full | Validation of a high-throughput and robust technique: BACs-on-beads assay (KaryoLite BoBs) for pre-implantation aneuploidy screening |
| title_fullStr | Validation of a high-throughput and robust technique: BACs-on-beads assay (KaryoLite BoBs) for pre-implantation aneuploidy screening |
| title_full_unstemmed | Validation of a high-throughput and robust technique: BACs-on-beads assay (KaryoLite BoBs) for pre-implantation aneuploidy screening |
| title_short | Validation of a high-throughput and robust technique: BACs-on-beads assay (KaryoLite BoBs) for pre-implantation aneuploidy screening |
| title_sort | validation of a high throughput and robust technique bacs on beads assay karyolite bobs for pre implantation aneuploidy screening |
| topic | BoB Karyolite aCGH PGS Aneuploidy |
| url | http://www.sciencedirect.com/science/article/pii/S1028455917301493 |
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