Comparison of immunogenic markers of human chondrocytes and chondroprogenitors derived from non-diseased and osteoarthritic articular cartilage
Background: In the field of cartilage repair, cell-based therapy, employing the use of mesenchymal stem cells and chondrocytes maybe a propitious treatment option. Once transferred, success of transplanted cells is determined by their immunogenicity making assessment imperative. Another contender in...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-06-01
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| Series: | Journal of Orthopaedics, Trauma and Rehabilitation |
| Online Access: | https://doi.org/10.1177/2210491720915927 |
| _version_ | 1819277932391038976 |
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| author | Elizabeth Vinod Boopalan Ramasamy Upasana Kachroo |
| author_facet | Elizabeth Vinod Boopalan Ramasamy Upasana Kachroo |
| author_sort | Elizabeth Vinod |
| collection | DOAJ |
| description | Background: In the field of cartilage repair, cell-based therapy, employing the use of mesenchymal stem cells and chondrocytes maybe a propitious treatment option. Once transferred, success of transplanted cells is determined by their immunogenicity making assessment imperative. Another contender in the field, may be articular chondroprogenitors (CPs) shown to possess chondrogenic potential and reduced expression of hypertrophy markers. Our aim was to assess immunogenic properties of CPs (non-diseased and osteoarthritic (OA)) and compare them with chondrocytes since it may be a good alternative for cell-based therapy and data regarding its immunogenic profile is limited. Methods: Human chondrocytes and CPs from the same cartilage source were isolated. Passage 0 cells characterized by fluorescence-activated cell sorting against human surface antigen were human leucocyte antigen class I (HLA-A2 and HLA-B7), HLA-DR and its costimulatory molecules (CD80 and CD86) and macrophage/monocyte marker (CD14). Results and conclusion: Our observations indicated that CPs isolated from human non-diseased and OA cartilage showed similar immunogenic properties to chondrocytes isolated from the same source with no significant difference in expression. High-to-moderate expression of MHC class I (HLA-A2 and HLA-B7) and moderate-to-low expression of MHC class II (HLA-DR and its co-stimulatory molecules CD80 and CD86) were observed. This is the first report to shed insight on the immunogenic properties of human cartilage-derived progenitors, a potential contender in the field of regenerative therapy for formation of genuine hyaline cartilage. |
| first_indexed | 2024-12-24T00:03:58Z |
| format | Article |
| id | doaj.art-d2be8f1a665e456abba24b75b78d74b0 |
| institution | Directory Open Access Journal |
| issn | 2210-4917 2210-4925 |
| language | English |
| last_indexed | 2024-12-24T00:03:58Z |
| publishDate | 2020-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Orthopaedics, Trauma and Rehabilitation |
| spelling | doaj.art-d2be8f1a665e456abba24b75b78d74b02022-12-21T17:25:03ZengSAGE PublishingJournal of Orthopaedics, Trauma and Rehabilitation2210-49172210-49252020-06-012710.1177/2210491720915927Comparison of immunogenic markers of human chondrocytes and chondroprogenitors derived from non-diseased and osteoarthritic articular cartilageElizabeth Vinod0Boopalan Ramasamy1Upasana Kachroo2 Centre for Stem Cell Research, Christian Medical College, Vellore, India Department of Orthopaedics, Royal Darwin Hospital, Tiwi, Australia Department of Physiology, Christian Medical College, Vellore, IndiaBackground: In the field of cartilage repair, cell-based therapy, employing the use of mesenchymal stem cells and chondrocytes maybe a propitious treatment option. Once transferred, success of transplanted cells is determined by their immunogenicity making assessment imperative. Another contender in the field, may be articular chondroprogenitors (CPs) shown to possess chondrogenic potential and reduced expression of hypertrophy markers. Our aim was to assess immunogenic properties of CPs (non-diseased and osteoarthritic (OA)) and compare them with chondrocytes since it may be a good alternative for cell-based therapy and data regarding its immunogenic profile is limited. Methods: Human chondrocytes and CPs from the same cartilage source were isolated. Passage 0 cells characterized by fluorescence-activated cell sorting against human surface antigen were human leucocyte antigen class I (HLA-A2 and HLA-B7), HLA-DR and its costimulatory molecules (CD80 and CD86) and macrophage/monocyte marker (CD14). Results and conclusion: Our observations indicated that CPs isolated from human non-diseased and OA cartilage showed similar immunogenic properties to chondrocytes isolated from the same source with no significant difference in expression. High-to-moderate expression of MHC class I (HLA-A2 and HLA-B7) and moderate-to-low expression of MHC class II (HLA-DR and its co-stimulatory molecules CD80 and CD86) were observed. This is the first report to shed insight on the immunogenic properties of human cartilage-derived progenitors, a potential contender in the field of regenerative therapy for formation of genuine hyaline cartilage.https://doi.org/10.1177/2210491720915927 |
| spellingShingle | Elizabeth Vinod Boopalan Ramasamy Upasana Kachroo Comparison of immunogenic markers of human chondrocytes and chondroprogenitors derived from non-diseased and osteoarthritic articular cartilage Journal of Orthopaedics, Trauma and Rehabilitation |
| title | Comparison of immunogenic markers of human chondrocytes and chondroprogenitors derived from non-diseased and osteoarthritic articular cartilage |
| title_full | Comparison of immunogenic markers of human chondrocytes and chondroprogenitors derived from non-diseased and osteoarthritic articular cartilage |
| title_fullStr | Comparison of immunogenic markers of human chondrocytes and chondroprogenitors derived from non-diseased and osteoarthritic articular cartilage |
| title_full_unstemmed | Comparison of immunogenic markers of human chondrocytes and chondroprogenitors derived from non-diseased and osteoarthritic articular cartilage |
| title_short | Comparison of immunogenic markers of human chondrocytes and chondroprogenitors derived from non-diseased and osteoarthritic articular cartilage |
| title_sort | comparison of immunogenic markers of human chondrocytes and chondroprogenitors derived from non diseased and osteoarthritic articular cartilage |
| url | https://doi.org/10.1177/2210491720915927 |
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