Cyclic nucleotides in archaea: Cyclic di‐AMP in the archaeon Haloferax volcanii and its putative role
Abstract The role of cyclic nucleotides as second messengers for intracellular signal transduction has been well described in bacteria. One recently discovered bacterial second messenger is cyclic di‐adenylate monophosphate (c‐di‐AMP), which has been demonstrated to be essential in bacteria. Compare...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-09-01
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| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.829 |
| _version_ | 1818902417062756352 |
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| author | Frank Braun Laura Thomalla Chris van derDoes Tessa E. F. Quax Thorsten Allers Volkhard Kaever Sonja‐Verena Albers |
| author_facet | Frank Braun Laura Thomalla Chris van derDoes Tessa E. F. Quax Thorsten Allers Volkhard Kaever Sonja‐Verena Albers |
| author_sort | Frank Braun |
| collection | DOAJ |
| description | Abstract The role of cyclic nucleotides as second messengers for intracellular signal transduction has been well described in bacteria. One recently discovered bacterial second messenger is cyclic di‐adenylate monophosphate (c‐di‐AMP), which has been demonstrated to be essential in bacteria. Compared to bacteria, significantly less is known about second messengers in archaea. This study presents the first evidence of in vivo presence of c‐di‐AMP in an archaeon. The model organism Haloferax volcanii was demonstrated to produce c‐di‐AMP. Its genome encodes one diadenylate cyclase (DacZ) which was shown to produce c‐di‐AMP in vitro. Similar to bacteria, the dacZ gene is essential and homologous overexpression of DacZ leads to cell death, suggesting the need for tight regulation of c‐di‐AMP levels. Such tight regulation often indicates the control of important regulatory processes. A central target of c‐di‐AMP signaling in bacteria is cellular osmohomeostasis. The results presented here suggest a comparable function in H. volcanii. A strain with decreased c‐di‐AMP levels exhibited an increased cell area in hypo‐salt medium, implying impaired osmoregulation. In summary, this study expands the field of research on c‐di‐AMP and its physiological function to archaea and indicates that osmoregulation is likely to be a common function of c‐di‐AMP in bacteria and archaea. |
| first_indexed | 2024-12-19T20:35:19Z |
| format | Article |
| id | doaj.art-d2c3afdeac1145f196428d284a8b9a2b |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-19T20:35:19Z |
| publishDate | 2019-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-d2c3afdeac1145f196428d284a8b9a2b2022-12-21T20:06:34ZengWileyMicrobiologyOpen2045-88272019-09-0189n/an/a10.1002/mbo3.829Cyclic nucleotides in archaea: Cyclic di‐AMP in the archaeon Haloferax volcanii and its putative roleFrank Braun0Laura Thomalla1Chris van derDoes2Tessa E. F. Quax3Thorsten Allers4Volkhard Kaever5Sonja‐Verena Albers6Molecular Biology of Archaea Institute of Biology, University of Freiburg Freiburg GermanyMolecular Biology of Archaea Institute of Biology, University of Freiburg Freiburg GermanyMolecular Biology of Archaea Institute of Biology, University of Freiburg Freiburg GermanyMolecular Biology of Archaea Institute of Biology, University of Freiburg Freiburg GermanySchool of Life Sciences, Queen's Medical Centre University of Nottingham Nottingham UKResearch Core Unit Metabolomics, Hannover Medical School Hannover GermanyMolecular Biology of Archaea Institute of Biology, University of Freiburg Freiburg GermanyAbstract The role of cyclic nucleotides as second messengers for intracellular signal transduction has been well described in bacteria. One recently discovered bacterial second messenger is cyclic di‐adenylate monophosphate (c‐di‐AMP), which has been demonstrated to be essential in bacteria. Compared to bacteria, significantly less is known about second messengers in archaea. This study presents the first evidence of in vivo presence of c‐di‐AMP in an archaeon. The model organism Haloferax volcanii was demonstrated to produce c‐di‐AMP. Its genome encodes one diadenylate cyclase (DacZ) which was shown to produce c‐di‐AMP in vitro. Similar to bacteria, the dacZ gene is essential and homologous overexpression of DacZ leads to cell death, suggesting the need for tight regulation of c‐di‐AMP levels. Such tight regulation often indicates the control of important regulatory processes. A central target of c‐di‐AMP signaling in bacteria is cellular osmohomeostasis. The results presented here suggest a comparable function in H. volcanii. A strain with decreased c‐di‐AMP levels exhibited an increased cell area in hypo‐salt medium, implying impaired osmoregulation. In summary, this study expands the field of research on c‐di‐AMP and its physiological function to archaea and indicates that osmoregulation is likely to be a common function of c‐di‐AMP in bacteria and archaea.https://doi.org/10.1002/mbo3.829archaeacyclic di‐AMPdiadenylate cyclaseHaloferax volcaniiosmoregulationsecond messenger |
| spellingShingle | Frank Braun Laura Thomalla Chris van derDoes Tessa E. F. Quax Thorsten Allers Volkhard Kaever Sonja‐Verena Albers Cyclic nucleotides in archaea: Cyclic di‐AMP in the archaeon Haloferax volcanii and its putative role MicrobiologyOpen archaea cyclic di‐AMP diadenylate cyclase Haloferax volcanii osmoregulation second messenger |
| title | Cyclic nucleotides in archaea: Cyclic di‐AMP in the archaeon Haloferax volcanii and its putative role |
| title_full | Cyclic nucleotides in archaea: Cyclic di‐AMP in the archaeon Haloferax volcanii and its putative role |
| title_fullStr | Cyclic nucleotides in archaea: Cyclic di‐AMP in the archaeon Haloferax volcanii and its putative role |
| title_full_unstemmed | Cyclic nucleotides in archaea: Cyclic di‐AMP in the archaeon Haloferax volcanii and its putative role |
| title_short | Cyclic nucleotides in archaea: Cyclic di‐AMP in the archaeon Haloferax volcanii and its putative role |
| title_sort | cyclic nucleotides in archaea cyclic di amp in the archaeon haloferax volcanii and its putative role |
| topic | archaea cyclic di‐AMP diadenylate cyclase Haloferax volcanii osmoregulation second messenger |
| url | https://doi.org/10.1002/mbo3.829 |
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