Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis
Context: Published data on genetic characterization of Toxoplasma gondii (T.gondii) from clinical cases of toxoplasmosis from India is lacking. Aims: The present study was aimed at identifying genetic types of T. gondii in fatal cases of cerebral toxoplasmosis (CT) associated with HIV, from India. S...
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Annals of Indian Academy of Neurology |
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| Online Access: | http://www.annalsofian.org/article.asp?issn=0972-2327;year=2018;volume=21;issue=4;spage=250;epage=255;aulast=Vijaykumar |
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| author | B R Vijaykumar R Sai Kant C Rajendran Swathi U Lekshmi Sundar Keerthana Anita Mahadevan S K Shankar R S Jayshree |
| author_facet | B R Vijaykumar R Sai Kant C Rajendran Swathi U Lekshmi Sundar Keerthana Anita Mahadevan S K Shankar R S Jayshree |
| author_sort | B R Vijaykumar |
| collection | DOAJ |
| description | Context: Published data on genetic characterization of Toxoplasma gondii (T.gondii) from clinical cases of toxoplasmosis from India is lacking. Aims: The present study was aimed at identifying genetic types of T. gondii in fatal cases of cerebral toxoplasmosis (CT) associated with HIV, from India. Settings and Design: Archived tissues of CT were obtained postmortem from 25 acquired immunodeficiency syndrome patients between 2000 and 2014. Subjects and Methods: Direct amplification of eight different loci, namely, SAG1, 5'-3'SAG2, Alt. SAG2, SAG3, BTUB, GRA6, C22-8, and L358 followed by restriction fragment length polymorphism was used to genotype the parasite. Results: The canonical Types I, II, or III were not found in our study. More than 96% of the cases harbored atypical genotypes–likely recombinants of the canonical types; one case closely corresponded to Type II genotype. Conclusions: Thus, a majority of T. gondii causing CT in South India belonged to a noncanonical lineage. These nonarchetypal genotypes differed from the conventional Types I, II, and III and caused devastating severity in patients with CT in the background of HIV. These results are a step further to deciphering the population genetics of this important zoonotic parasitic infection in Indian patients, information that has thus far been lacking. |
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| institution | Directory Open Access Journal |
| issn | 0972-2327 1998-3549 |
| language | English |
| last_indexed | 2024-12-16T06:23:44Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Annals of Indian Academy of Neurology |
| spelling | doaj.art-d2c63387c1d045048443e245f4eb26202022-12-21T22:41:04ZengWolters Kluwer Medknow PublicationsAnnals of Indian Academy of Neurology0972-23271998-35492018-01-0121425025510.4103/aian.AIAN_358_17Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosisB R VijaykumarR Sai KantC RajendranSwathi U LekshmiSundar KeerthanaAnita MahadevanS K ShankarR S JayshreeContext: Published data on genetic characterization of Toxoplasma gondii (T.gondii) from clinical cases of toxoplasmosis from India is lacking. Aims: The present study was aimed at identifying genetic types of T. gondii in fatal cases of cerebral toxoplasmosis (CT) associated with HIV, from India. Settings and Design: Archived tissues of CT were obtained postmortem from 25 acquired immunodeficiency syndrome patients between 2000 and 2014. Subjects and Methods: Direct amplification of eight different loci, namely, SAG1, 5'-3'SAG2, Alt. SAG2, SAG3, BTUB, GRA6, C22-8, and L358 followed by restriction fragment length polymorphism was used to genotype the parasite. Results: The canonical Types I, II, or III were not found in our study. More than 96% of the cases harbored atypical genotypes–likely recombinants of the canonical types; one case closely corresponded to Type II genotype. Conclusions: Thus, a majority of T. gondii causing CT in South India belonged to a noncanonical lineage. These nonarchetypal genotypes differed from the conventional Types I, II, and III and caused devastating severity in patients with CT in the background of HIV. These results are a step further to deciphering the population genetics of this important zoonotic parasitic infection in Indian patients, information that has thus far been lacking.http://www.annalsofian.org/article.asp?issn=0972-2327;year=2018;volume=21;issue=4;spage=250;epage=255;aulast=VijaykumarCerebral toxoplasmosisHIV/Acquired Immunodeficiency Syndrome patientsmultilocus polymerase chain reaction-restriction fragment length polymorphism genotypingToxoplasma gondii |
| spellingShingle | B R Vijaykumar R Sai Kant C Rajendran Swathi U Lekshmi Sundar Keerthana Anita Mahadevan S K Shankar R S Jayshree Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis Annals of Indian Academy of Neurology Cerebral toxoplasmosis HIV/Acquired Immunodeficiency Syndrome patients multilocus polymerase chain reaction-restriction fragment length polymorphism genotyping Toxoplasma gondii |
| title | Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis |
| title_full | Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis |
| title_fullStr | Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis |
| title_full_unstemmed | Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis |
| title_short | Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis |
| title_sort | restriction fragment length polymorphism based genotyping of toxoplasma gondii from autopsy proven cases of acquired immunodeficiency syndrome associated cerebral toxoplasmosis |
| topic | Cerebral toxoplasmosis HIV/Acquired Immunodeficiency Syndrome patients multilocus polymerase chain reaction-restriction fragment length polymorphism genotyping Toxoplasma gondii |
| url | http://www.annalsofian.org/article.asp?issn=0972-2327;year=2018;volume=21;issue=4;spage=250;epage=255;aulast=Vijaykumar |
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