Gut community alterations associated with Clostridioides difficile colonization in hospitalized gastroenterological patients with or without inflammatory bowel disease
Clostridioides difficile colonization and development of infection commonly occur in inflammatory bowel disease (IBD) patients and can trigger flare-ups. Both conditions are inherently linked to disrupted gut microbiota. This study included 149 hospitalized gastrointestinal patients, which were divi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.988426/full |
| _version_ | 1798031968419446784 |
|---|---|
| author | Aleksander Mahnic Spela Pintar Spela Pintar Pavel Skok Pavel Skok Maja Rupnik Maja Rupnik |
| author_facet | Aleksander Mahnic Spela Pintar Spela Pintar Pavel Skok Pavel Skok Maja Rupnik Maja Rupnik |
| author_sort | Aleksander Mahnic |
| collection | DOAJ |
| description | Clostridioides difficile colonization and development of infection commonly occur in inflammatory bowel disease (IBD) patients and can trigger flare-ups. Both conditions are inherently linked to disrupted gut microbiota. This study included 149 hospitalized gastrointestinal patients, which were divided into IBD (n = 48) and non-IBD patients (n = 101). Patients were tested for C. difficile colonization (qPCR and selective plating), and gut bacterial communities were analyzed with 16S amplicon sequencing. Blood test results were retrospectively collected from the medical records. IBD and non-IBD patients had comparable C. difficile colonization rates (31.7 and 33.3%, respectively). Compared to non-IBD C. difficile-non-colonized patients, IBD and C. difficile-colonized patients shared multiple common bacterial community characteristics including decreased diversity and reduced abundance of strict anaerobic bacteria. Furthermore, certain microbiota alterations were enhanced when IBD was accompanied by C. difficile colonization, indicating a synergistic effect between both medical complications. Conversely, certain microbial patterns were specific to C. difficile colonization, e.g., co-occurrence with Enterococcus, which was most common in IBD patients (81.3%). |
| first_indexed | 2024-04-11T20:05:08Z |
| format | Article |
| id | doaj.art-d2c960d15d354915987425d2f2e59cfa |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T20:05:08Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d2c960d15d354915987425d2f2e59cfa2022-12-22T04:05:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.988426988426Gut community alterations associated with Clostridioides difficile colonization in hospitalized gastroenterological patients with or without inflammatory bowel diseaseAleksander Mahnic0Spela Pintar1Spela Pintar2Pavel Skok3Pavel Skok4Maja Rupnik5Maja Rupnik6National Laboratory for Health, Environment and Food, Department for Microbiological Research, Maribor, SloveniaDepartment of Gastroenterology, University Medical Centre Ljubljana, Ljubljana, SloveniaFaculty of Medicine, University of Maribor, Maribor, SloveniaFaculty of Medicine, University of Maribor, Maribor, SloveniaDepartment of Gastroenterology, University Clinical Centre Maribor, Maribor, SloveniaNational Laboratory for Health, Environment and Food, Department for Microbiological Research, Maribor, SloveniaDepartment of Microbiology, Faculty of Medicine, University of Maribor, Maribor, SloveniaClostridioides difficile colonization and development of infection commonly occur in inflammatory bowel disease (IBD) patients and can trigger flare-ups. Both conditions are inherently linked to disrupted gut microbiota. This study included 149 hospitalized gastrointestinal patients, which were divided into IBD (n = 48) and non-IBD patients (n = 101). Patients were tested for C. difficile colonization (qPCR and selective plating), and gut bacterial communities were analyzed with 16S amplicon sequencing. Blood test results were retrospectively collected from the medical records. IBD and non-IBD patients had comparable C. difficile colonization rates (31.7 and 33.3%, respectively). Compared to non-IBD C. difficile-non-colonized patients, IBD and C. difficile-colonized patients shared multiple common bacterial community characteristics including decreased diversity and reduced abundance of strict anaerobic bacteria. Furthermore, certain microbiota alterations were enhanced when IBD was accompanied by C. difficile colonization, indicating a synergistic effect between both medical complications. Conversely, certain microbial patterns were specific to C. difficile colonization, e.g., co-occurrence with Enterococcus, which was most common in IBD patients (81.3%).https://www.frontiersin.org/articles/10.3389/fmicb.2022.988426/fullinflammatory bowel diseasegut microbiotaEnterococcus16S amplicon sequencingClostridium difficile |
| spellingShingle | Aleksander Mahnic Spela Pintar Spela Pintar Pavel Skok Pavel Skok Maja Rupnik Maja Rupnik Gut community alterations associated with Clostridioides difficile colonization in hospitalized gastroenterological patients with or without inflammatory bowel disease Frontiers in Microbiology inflammatory bowel disease gut microbiota Enterococcus 16S amplicon sequencing Clostridium difficile |
| title | Gut community alterations associated with Clostridioides difficile colonization in hospitalized gastroenterological patients with or without inflammatory bowel disease |
| title_full | Gut community alterations associated with Clostridioides difficile colonization in hospitalized gastroenterological patients with or without inflammatory bowel disease |
| title_fullStr | Gut community alterations associated with Clostridioides difficile colonization in hospitalized gastroenterological patients with or without inflammatory bowel disease |
| title_full_unstemmed | Gut community alterations associated with Clostridioides difficile colonization in hospitalized gastroenterological patients with or without inflammatory bowel disease |
| title_short | Gut community alterations associated with Clostridioides difficile colonization in hospitalized gastroenterological patients with or without inflammatory bowel disease |
| title_sort | gut community alterations associated with clostridioides difficile colonization in hospitalized gastroenterological patients with or without inflammatory bowel disease |
| topic | inflammatory bowel disease gut microbiota Enterococcus 16S amplicon sequencing Clostridium difficile |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.988426/full |
| work_keys_str_mv | AT aleksandermahnic gutcommunityalterationsassociatedwithclostridioidesdifficilecolonizationinhospitalizedgastroenterologicalpatientswithorwithoutinflammatoryboweldisease AT spelapintar gutcommunityalterationsassociatedwithclostridioidesdifficilecolonizationinhospitalizedgastroenterologicalpatientswithorwithoutinflammatoryboweldisease AT spelapintar gutcommunityalterationsassociatedwithclostridioidesdifficilecolonizationinhospitalizedgastroenterologicalpatientswithorwithoutinflammatoryboweldisease AT pavelskok gutcommunityalterationsassociatedwithclostridioidesdifficilecolonizationinhospitalizedgastroenterologicalpatientswithorwithoutinflammatoryboweldisease AT pavelskok gutcommunityalterationsassociatedwithclostridioidesdifficilecolonizationinhospitalizedgastroenterologicalpatientswithorwithoutinflammatoryboweldisease AT majarupnik gutcommunityalterationsassociatedwithclostridioidesdifficilecolonizationinhospitalizedgastroenterologicalpatientswithorwithoutinflammatoryboweldisease AT majarupnik gutcommunityalterationsassociatedwithclostridioidesdifficilecolonizationinhospitalizedgastroenterologicalpatientswithorwithoutinflammatoryboweldisease |