Experimental infection of post-weaned pigs with F18-encoding enterotoxigenic and enterotoxigenic/shigatoxigenic Escherichia coli stain isolated from the diarrheic feces in Korea
Background: Escherichia coli infections have been causing post-weaning diarrhea (PWD) and edema disease (ED) for many years in the porcine industry. In animals, it is classified into pathotypes and serotypes, according to virulence factors. Serotyping is performed for O, K, H, and F antigens, which...
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| Format: | Article |
| Language: | English |
| Published: |
Tripoli University
2023-06-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | https://www.openveterinaryjournal.com/?mno=148029 |
| _version_ | 1797657008656089088 |
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| author | Kang-Hyun Baek Warisraporn Tangchang Eun-Jin Choi Wan-Ky Lee Kyung-Hyun Lee Hyun-Kyung Lee Jae-Won Byun Hwa-Young Son |
| author_facet | Kang-Hyun Baek Warisraporn Tangchang Eun-Jin Choi Wan-Ky Lee Kyung-Hyun Lee Hyun-Kyung Lee Jae-Won Byun Hwa-Young Son |
| author_sort | Kang-Hyun Baek |
| collection | DOAJ |
| description | Background:
Escherichia coli infections have been causing post-weaning diarrhea (PWD) and edema disease (ED) for many years in the porcine industry. In animals, it is classified into pathotypes and serotypes, according to virulence factors. Serotyping is performed for O, K, H, and F antigens, which are important for discriminating pathogenicity and epidemiology. Furthermore, E. coli strains that produce F18 fimbriae are major sources of ED and PWD associated with Shiga-toxin producing E. coli (STEC) expressing F18ab, and enterotoxigenic E. coli (ETEC) expressing F18ac, respectively.
Aim:
To investigate the pathogenicity potential and infection characteristics of an experimental infection and confirm the pathological features of the Korean STEC/ETEC strains F18ab and F18ac in piglets.
Methods:
Three-week-old pigs were randomized into three experimental groups: infected G1 (F18ab), infected G2 (F18ac), and G3 (control). General health status was monitored daily, and pathological changes were evaluated.
Results:
Diarrhea occurred in all infected piglets. Pathological changes were only observed in the small intestine and regional lymph nodes. In G1, mucosal necrosis, inflammatory cell infiltration with hemorrhagic lesions, and apoptotic cell death in the tunica media of arterioles in the small intestine were observed. In contrast, the mucosa and epithelium appeared almost intact, with no abnormal vessel lesions in G2.
Conclusions:
Both strains which isolated from pigs in Korea could be infected and did not spread from the alimentary tract to other organs. The pathological features were quite different among the F18 subtypes. The F18ab strain was more virulent than F18ac, and virulence characteristics of the F18ac strain were more similar to ETEC than to STEC. [Open Vet J 2023; 13(6.000): 705-714] |
| first_indexed | 2024-03-11T17:38:14Z |
| format | Article |
| id | doaj.art-d2ca4e0ec58c40fd8d9f9dc89575a065 |
| institution | Directory Open Access Journal |
| issn | 2226-4485 |
| language | English |
| last_indexed | 2024-03-11T17:38:14Z |
| publishDate | 2023-06-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-d2ca4e0ec58c40fd8d9f9dc89575a0652023-10-18T15:29:29ZengTripoli UniversityOpen Veterinary Journal2226-44852023-06-0113670571410.5455/OVJ.2023.v13.i6.5148029Experimental infection of post-weaned pigs with F18-encoding enterotoxigenic and enterotoxigenic/shigatoxigenic Escherichia coli stain isolated from the diarrheic feces in KoreaKang-Hyun Baek0Warisraporn Tangchang1Eun-Jin Choi2Wan-Ky Lee3Kyung-Hyun Lee4Hyun-Kyung Lee5Jae-Won Byun6Hwa-Young Son7177, Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea. 99, Daehak-ro, Yuseong-gu, Daejeon-si 34134, Republic of Korea. 177, Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea. Chungdae-ro 1, Seowon-Gu, Cheongju-si, Chungcheongbuk-do 28644, Republic of Korea. 177, Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea. 177, Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea. 177, Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea. 99, Daehak-ro, Yuseong-gu, Daejeon-si 34134, Republic of Korea.Background: Escherichia coli infections have been causing post-weaning diarrhea (PWD) and edema disease (ED) for many years in the porcine industry. In animals, it is classified into pathotypes and serotypes, according to virulence factors. Serotyping is performed for O, K, H, and F antigens, which are important for discriminating pathogenicity and epidemiology. Furthermore, E. coli strains that produce F18 fimbriae are major sources of ED and PWD associated with Shiga-toxin producing E. coli (STEC) expressing F18ab, and enterotoxigenic E. coli (ETEC) expressing F18ac, respectively. Aim: To investigate the pathogenicity potential and infection characteristics of an experimental infection and confirm the pathological features of the Korean STEC/ETEC strains F18ab and F18ac in piglets. Methods: Three-week-old pigs were randomized into three experimental groups: infected G1 (F18ab), infected G2 (F18ac), and G3 (control). General health status was monitored daily, and pathological changes were evaluated. Results: Diarrhea occurred in all infected piglets. Pathological changes were only observed in the small intestine and regional lymph nodes. In G1, mucosal necrosis, inflammatory cell infiltration with hemorrhagic lesions, and apoptotic cell death in the tunica media of arterioles in the small intestine were observed. In contrast, the mucosa and epithelium appeared almost intact, with no abnormal vessel lesions in G2. Conclusions: Both strains which isolated from pigs in Korea could be infected and did not spread from the alimentary tract to other organs. The pathological features were quite different among the F18 subtypes. The F18ab strain was more virulent than F18ac, and virulence characteristics of the F18ac strain were more similar to ETEC than to STEC. [Open Vet J 2023; 13(6.000): 705-714]https://www.openveterinaryjournal.com/?mno=148029edema diseaseenteropathogenic escherichia colif18 variantskorean stainspost-weaning diarrhea |
| spellingShingle | Kang-Hyun Baek Warisraporn Tangchang Eun-Jin Choi Wan-Ky Lee Kyung-Hyun Lee Hyun-Kyung Lee Jae-Won Byun Hwa-Young Son Experimental infection of post-weaned pigs with F18-encoding enterotoxigenic and enterotoxigenic/shigatoxigenic Escherichia coli stain isolated from the diarrheic feces in Korea Open Veterinary Journal edema disease enteropathogenic escherichia coli f18 variants korean stains post-weaning diarrhea |
| title | Experimental infection of post-weaned pigs with F18-encoding enterotoxigenic and enterotoxigenic/shigatoxigenic Escherichia coli stain isolated from the diarrheic feces in Korea |
| title_full | Experimental infection of post-weaned pigs with F18-encoding enterotoxigenic and enterotoxigenic/shigatoxigenic Escherichia coli stain isolated from the diarrheic feces in Korea |
| title_fullStr | Experimental infection of post-weaned pigs with F18-encoding enterotoxigenic and enterotoxigenic/shigatoxigenic Escherichia coli stain isolated from the diarrheic feces in Korea |
| title_full_unstemmed | Experimental infection of post-weaned pigs with F18-encoding enterotoxigenic and enterotoxigenic/shigatoxigenic Escherichia coli stain isolated from the diarrheic feces in Korea |
| title_short | Experimental infection of post-weaned pigs with F18-encoding enterotoxigenic and enterotoxigenic/shigatoxigenic Escherichia coli stain isolated from the diarrheic feces in Korea |
| title_sort | experimental infection of post weaned pigs with f18 encoding enterotoxigenic and enterotoxigenic shigatoxigenic escherichia coli stain isolated from the diarrheic feces in korea |
| topic | edema disease enteropathogenic escherichia coli f18 variants korean stains post-weaning diarrhea |
| url | https://www.openveterinaryjournal.com/?mno=148029 |
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