In Situ Detection of Complex DNA Damage Using Microscopy: A Rough Road Ahead
Complexity of DNA damage is considered currently one if not the primary instigator of biological responses and determinant of short and long-term effects in organisms and their offspring. In this review, we focus on the detection of complex (clustered) DNA damage (CDD) induced for example by ionizin...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/12/11/3288 |
| _version_ | 1797548707107831808 |
|---|---|
| author | Zacharenia Nikitaki Eloise Pariset Damir Sudar Sylvain V. Costes Alexandros G. Georgakilas |
| author_facet | Zacharenia Nikitaki Eloise Pariset Damir Sudar Sylvain V. Costes Alexandros G. Georgakilas |
| author_sort | Zacharenia Nikitaki |
| collection | DOAJ |
| description | Complexity of DNA damage is considered currently one if not the primary instigator of biological responses and determinant of short and long-term effects in organisms and their offspring. In this review, we focus on the detection of complex (clustered) DNA damage (CDD) induced for example by ionizing radiation (IR) and in some cases by high oxidative stress. We perform a short historical perspective in the field, emphasizing the microscopy-based techniques and methodologies for the detection of CDD at the cellular level. We extend this analysis on the pertaining methodology of surrogate protein markers of CDD (foci) colocalization and provide a unique synthesis of imaging parameters, software, and different types of microscopy used. Last but not least, we critically discuss the main advances and necessary future direction for the better detection of CDD, with important outcomes in biological and clinical setups. |
| first_indexed | 2024-03-10T15:03:28Z |
| format | Article |
| id | doaj.art-d2ccdb9a9be54dd4821122fc7e00044d |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T15:03:28Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-d2ccdb9a9be54dd4821122fc7e00044d2023-11-20T20:01:15ZengMDPI AGCancers2072-66942020-11-011211328810.3390/cancers12113288In Situ Detection of Complex DNA Damage Using Microscopy: A Rough Road AheadZacharenia Nikitaki0Eloise Pariset1Damir Sudar2Sylvain V. Costes3Alexandros G. Georgakilas4Physics Department, School of Applied Mathematical and Physical Sciences, DNA Damage Laboratory, National Technical University of Athens (NTUA), 15780 Zografou, Athens, GreeceSpace Biosciences Division, Radiation Biophysics Laboratory, NASA Ames Research Center, Moffett Field, CA 94035, USALife Sciences Department, Quantitative Imaging Systems LLC, Portland, OR 97209, USASpace Biosciences Division, Radiation Biophysics Laboratory, NASA Ames Research Center, Moffett Field, CA 94035, USAPhysics Department, School of Applied Mathematical and Physical Sciences, DNA Damage Laboratory, National Technical University of Athens (NTUA), 15780 Zografou, Athens, GreeceComplexity of DNA damage is considered currently one if not the primary instigator of biological responses and determinant of short and long-term effects in organisms and their offspring. In this review, we focus on the detection of complex (clustered) DNA damage (CDD) induced for example by ionizing radiation (IR) and in some cases by high oxidative stress. We perform a short historical perspective in the field, emphasizing the microscopy-based techniques and methodologies for the detection of CDD at the cellular level. We extend this analysis on the pertaining methodology of surrogate protein markers of CDD (foci) colocalization and provide a unique synthesis of imaging parameters, software, and different types of microscopy used. Last but not least, we critically discuss the main advances and necessary future direction for the better detection of CDD, with important outcomes in biological and clinical setups.https://www.mdpi.com/2072-6694/12/11/3288colocalizationcomplex DNA damagedetection of DNA damagefluorescence microscopydSTORMsingle-molecule detection |
| spellingShingle | Zacharenia Nikitaki Eloise Pariset Damir Sudar Sylvain V. Costes Alexandros G. Georgakilas In Situ Detection of Complex DNA Damage Using Microscopy: A Rough Road Ahead Cancers colocalization complex DNA damage detection of DNA damage fluorescence microscopy dSTORM single-molecule detection |
| title | In Situ Detection of Complex DNA Damage Using Microscopy: A Rough Road Ahead |
| title_full | In Situ Detection of Complex DNA Damage Using Microscopy: A Rough Road Ahead |
| title_fullStr | In Situ Detection of Complex DNA Damage Using Microscopy: A Rough Road Ahead |
| title_full_unstemmed | In Situ Detection of Complex DNA Damage Using Microscopy: A Rough Road Ahead |
| title_short | In Situ Detection of Complex DNA Damage Using Microscopy: A Rough Road Ahead |
| title_sort | in situ detection of complex dna damage using microscopy a rough road ahead |
| topic | colocalization complex DNA damage detection of DNA damage fluorescence microscopy dSTORM single-molecule detection |
| url | https://www.mdpi.com/2072-6694/12/11/3288 |
| work_keys_str_mv | AT zacharenianikitaki insitudetectionofcomplexdnadamageusingmicroscopyaroughroadahead AT eloisepariset insitudetectionofcomplexdnadamageusingmicroscopyaroughroadahead AT damirsudar insitudetectionofcomplexdnadamageusingmicroscopyaroughroadahead AT sylvainvcostes insitudetectionofcomplexdnadamageusingmicroscopyaroughroadahead AT alexandrosggeorgakilas insitudetectionofcomplexdnadamageusingmicroscopyaroughroadahead |