Development of Low-Serum Culture Media for the in Vitro Cultivation of Theileria annulata S15 Cell Line
Background: The production of bovine theileriosis vaccine involves in vitro cultivation of Theileria annulata schizont-infected cell lines. Fetal bovine serum (FBS) is commonly used in animal cell culture, including the Theileria cell line. However, we aimed to reduce the amount of serum needed for...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2024-03-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3949 |
| _version_ | 1797266453649424384 |
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| author | Gholamreza Habibi Asghar Afshari Amin Shahedi Mousa Hashemlo Ali Es-Haghi Mohamad Hosein Fallah Mehrabadi Saeid Fathi |
| author_facet | Gholamreza Habibi Asghar Afshari Amin Shahedi Mousa Hashemlo Ali Es-Haghi Mohamad Hosein Fallah Mehrabadi Saeid Fathi |
| author_sort | Gholamreza Habibi |
| collection | DOAJ |
| description |
Background: The production of bovine theileriosis vaccine involves in vitro cultivation of Theileria annulata schizont-infected cell lines. Fetal bovine serum (FBS) is commonly used in animal cell culture, including the Theileria cell line. However, we aimed to reduce the amount of serum needed for cell culture by modifying the Stoker culture medium with supplements such as excretion factor and serum substitutes.
Methods: To evaluate the effectiveness of these modifications, techniques such as cell counting, cell viability assays, and genomic analysis were employed in the Parasitic Vaccines Production Department of Razi institute of Iran, from 2020 to 2022. Statistical analysis was used to compare the results of different experimental conditions.
Results: The three experimental media were as effective as the commonly used 10% Stoker medium in supporting the growth and viability of cells.
Conclusion: The significant reduction in the required amount of serum and the remarkable cell growth achieved by using defined serum replacements for the production of cell culture media is a significant step towards the preparation of a proper cell culture medium for the production of bovine Theileriosis vaccine.
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| first_indexed | 2024-04-25T01:00:56Z |
| format | Article |
| id | doaj.art-d2cf93a1a3f943119d643c946603b346 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-04-25T01:00:56Z |
| publishDate | 2024-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-d2cf93a1a3f943119d643c946603b3462024-03-11T05:30:01ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2024-03-01191Development of Low-Serum Culture Media for the in Vitro Cultivation of Theileria annulata S15 Cell LineGholamreza Habibi0Asghar Afshari1Amin Shahedi2Mousa Hashemlo3Ali Es-Haghi4Mohamad Hosein Fallah Mehrabadi5Saeid Fathi6Department of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agricul-ture Research, Education and Extension Organization, Karaj, IranDepartment of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agricul-ture Research, Education and Extension Organization, Karaj, IranDepartment of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agricul-ture Research, Education and Extension Organization, Karaj, IranDepartment of Veterinary Biological Products Approval Group of Razi Vaccine and Serum Research, Agricultural Research, Education and Extension Organization, Karaj, IranDepartment of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, IranDepartment of Epidemiology, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization, Karaj, IranDepartment of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization, Karaj, Iran Background: The production of bovine theileriosis vaccine involves in vitro cultivation of Theileria annulata schizont-infected cell lines. Fetal bovine serum (FBS) is commonly used in animal cell culture, including the Theileria cell line. However, we aimed to reduce the amount of serum needed for cell culture by modifying the Stoker culture medium with supplements such as excretion factor and serum substitutes. Methods: To evaluate the effectiveness of these modifications, techniques such as cell counting, cell viability assays, and genomic analysis were employed in the Parasitic Vaccines Production Department of Razi institute of Iran, from 2020 to 2022. Statistical analysis was used to compare the results of different experimental conditions. Results: The three experimental media were as effective as the commonly used 10% Stoker medium in supporting the growth and viability of cells. Conclusion: The significant reduction in the required amount of serum and the remarkable cell growth achieved by using defined serum replacements for the production of cell culture media is a significant step towards the preparation of a proper cell culture medium for the production of bovine Theileriosis vaccine. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3949StokerCell culture mediumTheileria annulataSerum |
| spellingShingle | Gholamreza Habibi Asghar Afshari Amin Shahedi Mousa Hashemlo Ali Es-Haghi Mohamad Hosein Fallah Mehrabadi Saeid Fathi Development of Low-Serum Culture Media for the in Vitro Cultivation of Theileria annulata S15 Cell Line Iranian Journal of Parasitology Stoker Cell culture medium Theileria annulata Serum |
| title | Development of Low-Serum Culture Media for the in Vitro Cultivation of Theileria annulata S15 Cell Line |
| title_full | Development of Low-Serum Culture Media for the in Vitro Cultivation of Theileria annulata S15 Cell Line |
| title_fullStr | Development of Low-Serum Culture Media for the in Vitro Cultivation of Theileria annulata S15 Cell Line |
| title_full_unstemmed | Development of Low-Serum Culture Media for the in Vitro Cultivation of Theileria annulata S15 Cell Line |
| title_short | Development of Low-Serum Culture Media for the in Vitro Cultivation of Theileria annulata S15 Cell Line |
| title_sort | development of low serum culture media for the in vitro cultivation of theileria annulata s15 cell line |
| topic | Stoker Cell culture medium Theileria annulata Serum |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3949 |
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