Alterations in gut microbiota and metabolite profiles in patients with infantile cholestasis
Abstract Background Infantile cholestasis (IC) is the most common hepatobiliary disease in infants, resulting in elevated direct bilirubin levels. Indeed, hepatointestinal circulation impacts bile acid and bilirubin metabolism. This study evaluates changes in the gut microbiota composition in childr...
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-03115-1 |
| _version_ | 1797577775848095744 |
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| author | Meng Jin Jinghua Cui Huijuan Ning Meijuan Wang Wenwen Liu Kunyu Yao Jing Yuan Xuemei Zhong |
| author_facet | Meng Jin Jinghua Cui Huijuan Ning Meijuan Wang Wenwen Liu Kunyu Yao Jing Yuan Xuemei Zhong |
| author_sort | Meng Jin |
| collection | DOAJ |
| description | Abstract Background Infantile cholestasis (IC) is the most common hepatobiliary disease in infants, resulting in elevated direct bilirubin levels. Indeed, hepatointestinal circulation impacts bile acid and bilirubin metabolism. This study evaluates changes in the gut microbiota composition in children with IC and identifies abnormal metabolite profiles associated with microbial alterations. Results The gut microbiota in the IC group exhibits the higher abundance of Veillonella, Streptococcus and Clostridium spp. (P < 0.05), compared to healthy infants (CON) group. Moreover, the abundance of Ruminococcus, Vibrio butyricum, Eubacterium coprostanogenes group, Intestinibacter, and Faecalibacterium were lower (P < 0.05). In terms of microbiota-derived metabolites, the levels of fatty acids (palmitoleic, α-linolenic, arachidonic, and linoleic) (P < 0.05) increased and the levels of amino acids decreased in IC group. Furthermore, the abundances of Ruminococcus, Eubacterium coprostanoligenes group, Intestinibacter and Butyrivibrio are positively correlated with proline, asparagine and aspartic acid, but negatively correlated with the α-linolenic acid, linoleic acid, palmitoleic acid and arachidonic acid. For analysis of the relationship between the microbiota and clinical index, it was found that the abundance of Veillonella and Streptococcus was positively correlated with serum bile acid content (P < 0.05), while APTT, PT and INR were negatively correlated with Faecalibalum and Ruminococcus (P < 0.05). Conclusion Microbiota dysbiosis happened in IC children, which also can lead to the abnormal metabolism, thus obstructing the absorption of enteral nutrition and aggravating liver cell damage. Veillonella, Ruminococcus and Butyrivibrio may be important microbiome related with IC and need further research. |
| first_indexed | 2024-03-10T22:13:45Z |
| format | Article |
| id | doaj.art-d2d044d7a605472a8d4c6c2736c5a558 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-10T22:13:45Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-d2d044d7a605472a8d4c6c2736c5a5582023-11-19T12:32:41ZengBMCBMC Microbiology1471-21802023-11-0123111210.1186/s12866-023-03115-1Alterations in gut microbiota and metabolite profiles in patients with infantile cholestasisMeng Jin0Jinghua Cui1Huijuan Ning2Meijuan Wang3Wenwen Liu4Kunyu Yao5Jing Yuan6Xuemei Zhong7Gastroenterology Department, Children’s Hospital Capital Institute of PediatricsBacteriology Department, Capital Institute of PediatricsGastroenterology Department, Children’s Hospital Capital Institute of PediatricsGastroenterology Department, Children’s Hospital Capital Institute of PediatricsGastroenterology Department, Children’s Hospital Capital Institute of PediatricsGastroenterology Department, Children’s Hospital Capital Institute of PediatricsBacteriology Department, Capital Institute of PediatricsGastroenterology Department, Children’s Hospital Capital Institute of PediatricsAbstract Background Infantile cholestasis (IC) is the most common hepatobiliary disease in infants, resulting in elevated direct bilirubin levels. Indeed, hepatointestinal circulation impacts bile acid and bilirubin metabolism. This study evaluates changes in the gut microbiota composition in children with IC and identifies abnormal metabolite profiles associated with microbial alterations. Results The gut microbiota in the IC group exhibits the higher abundance of Veillonella, Streptococcus and Clostridium spp. (P < 0.05), compared to healthy infants (CON) group. Moreover, the abundance of Ruminococcus, Vibrio butyricum, Eubacterium coprostanogenes group, Intestinibacter, and Faecalibacterium were lower (P < 0.05). In terms of microbiota-derived metabolites, the levels of fatty acids (palmitoleic, α-linolenic, arachidonic, and linoleic) (P < 0.05) increased and the levels of amino acids decreased in IC group. Furthermore, the abundances of Ruminococcus, Eubacterium coprostanoligenes group, Intestinibacter and Butyrivibrio are positively correlated with proline, asparagine and aspartic acid, but negatively correlated with the α-linolenic acid, linoleic acid, palmitoleic acid and arachidonic acid. For analysis of the relationship between the microbiota and clinical index, it was found that the abundance of Veillonella and Streptococcus was positively correlated with serum bile acid content (P < 0.05), while APTT, PT and INR were negatively correlated with Faecalibalum and Ruminococcus (P < 0.05). Conclusion Microbiota dysbiosis happened in IC children, which also can lead to the abnormal metabolism, thus obstructing the absorption of enteral nutrition and aggravating liver cell damage. Veillonella, Ruminococcus and Butyrivibrio may be important microbiome related with IC and need further research.https://doi.org/10.1186/s12866-023-03115-1Infantile cholestasis (IC)Gut microbiotaMicrobiota-derived metabolitesRuminococcusButyrivibrioVeillonella |
| spellingShingle | Meng Jin Jinghua Cui Huijuan Ning Meijuan Wang Wenwen Liu Kunyu Yao Jing Yuan Xuemei Zhong Alterations in gut microbiota and metabolite profiles in patients with infantile cholestasis BMC Microbiology Infantile cholestasis (IC) Gut microbiota Microbiota-derived metabolites Ruminococcus Butyrivibrio Veillonella |
| title | Alterations in gut microbiota and metabolite profiles in patients with infantile cholestasis |
| title_full | Alterations in gut microbiota and metabolite profiles in patients with infantile cholestasis |
| title_fullStr | Alterations in gut microbiota and metabolite profiles in patients with infantile cholestasis |
| title_full_unstemmed | Alterations in gut microbiota and metabolite profiles in patients with infantile cholestasis |
| title_short | Alterations in gut microbiota and metabolite profiles in patients with infantile cholestasis |
| title_sort | alterations in gut microbiota and metabolite profiles in patients with infantile cholestasis |
| topic | Infantile cholestasis (IC) Gut microbiota Microbiota-derived metabolites Ruminococcus Butyrivibrio Veillonella |
| url | https://doi.org/10.1186/s12866-023-03115-1 |
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