Gut Microbiota and Metabolic Endotoxemia in Young Obese Mexican Subjects
Background: The gut microbiota plays an important role in human metabolism; previous studies suggest that the imbalance can cause a metabolic endotoxemia that may be linked to weight gain and insulin resistance. The purpose of this study was to investigate the relationship between the gut microbiota...
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| Format: | Article |
| Language: | English |
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Karger Publishers
2016-01-01
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| Series: | Obesity Facts |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/442479 |
| _version_ | 1818931640481611776 |
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| author | Romina Belén Radilla-Vázquez Isela Parra-Rojas Norma Edith Martínez-Hernández Yolanda Fabiola Márquez-Sandoval Berenice Illades-Aguiar Natividad Castro-Alarcón |
| author_facet | Romina Belén Radilla-Vázquez Isela Parra-Rojas Norma Edith Martínez-Hernández Yolanda Fabiola Márquez-Sandoval Berenice Illades-Aguiar Natividad Castro-Alarcón |
| author_sort | Romina Belén Radilla-Vázquez |
| collection | DOAJ |
| description | Background: The gut microbiota plays an important role in human metabolism; previous studies suggest that the imbalance can cause a metabolic endotoxemia that may be linked to weight gain and insulin resistance. The purpose of this study was to investigate the relationship between the gut microbiota composition, the lipopolysaccharide levels and the metabolic profile in obese and normal-weight young subjects. Methods: We studied 32 obese (BMI ≥ 30 kg/m2) and 32 normal-weight subjects (BMI = 18.5-24.9 kg/m2), aged 18-25 years. Quantification of intestinal bacteria was performed by real-time PCR. Endotoxin units were determined with the test QCL-1000, and biochemical profile was performed under a standard protocol of Spinreact. Results: Obese individuals had a BMI of 34.5 (32.9-36.45) kg/m2, increased triglycerides (123 vs. 70 mg/dl), total cholesterol (168 vs. 142 mg/dl), and LDL-cholesterol (114 vs. 96.5 mg/dl). In obese subjects body temperature was higher than in normal-weight subjects. We found a greater number of Clostridum leptum and Lactobacillus (p Prevotella and Escherichia coli (p E. coli was associated with an increased risk of lipopolysaccharide levels ranging from 1 to 1.3 EU/ml. A positive correlation was found between serum lipopolysaccharides and BMI (r = 0.46, p = 0.008), triglyceride levels (r = 0.44, p = 0.011) as well as waist circumference (r = 0.34, p = 0.040), being more evident in young obese females. Conclusion: Subclinical metabolic endotoxemia determined by serum concentration of lipopolysaccharides was related to the smallest amount of E. coli, high triglyceride levels, and central adiposity in obese young persons. |
| first_indexed | 2024-12-20T04:19:48Z |
| format | Article |
| id | doaj.art-d2d0ab60a9674d1d8e0a32b314c220b5 |
| institution | Directory Open Access Journal |
| issn | 1662-4025 1662-4033 |
| language | English |
| last_indexed | 2024-12-20T04:19:48Z |
| publishDate | 2016-01-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Obesity Facts |
| spelling | doaj.art-d2d0ab60a9674d1d8e0a32b314c220b52022-12-21T19:53:39ZengKarger PublishersObesity Facts1662-40251662-40332016-01-019111110.1159/000442479442479Gut Microbiota and Metabolic Endotoxemia in Young Obese Mexican SubjectsRomina Belén Radilla-VázquezIsela Parra-RojasNorma Edith Martínez-HernándezYolanda Fabiola Márquez-SandovalBerenice Illades-AguiarNatividad Castro-AlarcónBackground: The gut microbiota plays an important role in human metabolism; previous studies suggest that the imbalance can cause a metabolic endotoxemia that may be linked to weight gain and insulin resistance. The purpose of this study was to investigate the relationship between the gut microbiota composition, the lipopolysaccharide levels and the metabolic profile in obese and normal-weight young subjects. Methods: We studied 32 obese (BMI ≥ 30 kg/m2) and 32 normal-weight subjects (BMI = 18.5-24.9 kg/m2), aged 18-25 years. Quantification of intestinal bacteria was performed by real-time PCR. Endotoxin units were determined with the test QCL-1000, and biochemical profile was performed under a standard protocol of Spinreact. Results: Obese individuals had a BMI of 34.5 (32.9-36.45) kg/m2, increased triglycerides (123 vs. 70 mg/dl), total cholesterol (168 vs. 142 mg/dl), and LDL-cholesterol (114 vs. 96.5 mg/dl). In obese subjects body temperature was higher than in normal-weight subjects. We found a greater number of Clostridum leptum and Lactobacillus (p Prevotella and Escherichia coli (p E. coli was associated with an increased risk of lipopolysaccharide levels ranging from 1 to 1.3 EU/ml. A positive correlation was found between serum lipopolysaccharides and BMI (r = 0.46, p = 0.008), triglyceride levels (r = 0.44, p = 0.011) as well as waist circumference (r = 0.34, p = 0.040), being more evident in young obese females. Conclusion: Subclinical metabolic endotoxemia determined by serum concentration of lipopolysaccharides was related to the smallest amount of E. coli, high triglyceride levels, and central adiposity in obese young persons.http://www.karger.com/Article/FullText/442479Gut microbiotaMetabolic endotoxemiaObesity |
| spellingShingle | Romina Belén Radilla-Vázquez Isela Parra-Rojas Norma Edith Martínez-Hernández Yolanda Fabiola Márquez-Sandoval Berenice Illades-Aguiar Natividad Castro-Alarcón Gut Microbiota and Metabolic Endotoxemia in Young Obese Mexican Subjects Obesity Facts Gut microbiota Metabolic endotoxemia Obesity |
| title | Gut Microbiota and Metabolic Endotoxemia in Young Obese Mexican Subjects |
| title_full | Gut Microbiota and Metabolic Endotoxemia in Young Obese Mexican Subjects |
| title_fullStr | Gut Microbiota and Metabolic Endotoxemia in Young Obese Mexican Subjects |
| title_full_unstemmed | Gut Microbiota and Metabolic Endotoxemia in Young Obese Mexican Subjects |
| title_short | Gut Microbiota and Metabolic Endotoxemia in Young Obese Mexican Subjects |
| title_sort | gut microbiota and metabolic endotoxemia in young obese mexican subjects |
| topic | Gut microbiota Metabolic endotoxemia Obesity |
| url | http://www.karger.com/Article/FullText/442479 |
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