Polysaccharides from Radix <i>Peucedani</i>: Extraction, Structural Characterization and Antioxidant Activity
In this study, an ultrasound-assisted green extraction method was applied for the extraction of polysaccharides from Radix <i>Peucedani</i> based on deep eutectic solvents (DESs), and the result showed that a DES system composed of betaine and 1,2-propylene glycol with a molar ratio of 1...
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MDPI AG
2023-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/23/7845 |
| _version_ | 1827592203864637440 |
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| author | Jie Zhang Chenyue Wang Qian Li Wei Liang |
| author_facet | Jie Zhang Chenyue Wang Qian Li Wei Liang |
| author_sort | Jie Zhang |
| collection | DOAJ |
| description | In this study, an ultrasound-assisted green extraction method was applied for the extraction of polysaccharides from Radix <i>Peucedani</i> based on deep eutectic solvents (DESs), and the result showed that a DES system composed of betaine and 1,2-propylene glycol with a molar ratio of 1:2 possessed the optimal extraction efficiency for polysaccharides. Single-factor and Box–Behnken designs were used to determine the optimum extraction conditions for the maximum yields of polysaccharides from Radix <i>Peucedani</i> by using DESs. The maximum yields of polysaccharides attained 11.372% within a DES water content of 19%, an extraction time of 36 min, an extraction temperature of 54 °C, a solid–liquid ratio of 1:30 and an ultrasonic irradiation power of 420 W. The physicochemical properties of polysaccharides were analyzed using ICS and FT-IR, and the structure morphology was observed by SEM. The polysaccharides extracted from Radix <i>Peucedani</i> exhibited general antioxidant activities in vitro including DPPH, Hydroxyl and ABTS+ radical-scavenging activity. The antioxidant mechanism of Radix <i>Peucedani</i> polysaccharides was investigated using network pharmacology and molecular docking methods. The result showed that the high binding activity of glucose and IL1B, galactose and CASP3 was recognized as a potential mechanism for the antioxidant effects of Radix <i>Peucedani</i> polysaccharides. |
| first_indexed | 2024-03-09T01:46:22Z |
| format | Article |
| id | doaj.art-d2d51ee72d27452ab36a9a0df3f12830 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T01:46:22Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d2d51ee72d27452ab36a9a0df3f128302023-12-08T15:22:31ZengMDPI AGMolecules1420-30492023-11-012823784510.3390/molecules28237845Polysaccharides from Radix <i>Peucedani</i>: Extraction, Structural Characterization and Antioxidant ActivityJie Zhang0Chenyue Wang1Qian Li2Wei Liang3State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaIn this study, an ultrasound-assisted green extraction method was applied for the extraction of polysaccharides from Radix <i>Peucedani</i> based on deep eutectic solvents (DESs), and the result showed that a DES system composed of betaine and 1,2-propylene glycol with a molar ratio of 1:2 possessed the optimal extraction efficiency for polysaccharides. Single-factor and Box–Behnken designs were used to determine the optimum extraction conditions for the maximum yields of polysaccharides from Radix <i>Peucedani</i> by using DESs. The maximum yields of polysaccharides attained 11.372% within a DES water content of 19%, an extraction time of 36 min, an extraction temperature of 54 °C, a solid–liquid ratio of 1:30 and an ultrasonic irradiation power of 420 W. The physicochemical properties of polysaccharides were analyzed using ICS and FT-IR, and the structure morphology was observed by SEM. The polysaccharides extracted from Radix <i>Peucedani</i> exhibited general antioxidant activities in vitro including DPPH, Hydroxyl and ABTS+ radical-scavenging activity. The antioxidant mechanism of Radix <i>Peucedani</i> polysaccharides was investigated using network pharmacology and molecular docking methods. The result showed that the high binding activity of glucose and IL1B, galactose and CASP3 was recognized as a potential mechanism for the antioxidant effects of Radix <i>Peucedani</i> polysaccharides.https://www.mdpi.com/1420-3049/28/23/7845Radix <i>Peucedani</i>polysaccharidesdeep eutectic solventsnetwork pharmacologymolecular dockingantioxidant |
| spellingShingle | Jie Zhang Chenyue Wang Qian Li Wei Liang Polysaccharides from Radix <i>Peucedani</i>: Extraction, Structural Characterization and Antioxidant Activity Molecules Radix <i>Peucedani</i> polysaccharides deep eutectic solvents network pharmacology molecular docking antioxidant |
| title | Polysaccharides from Radix <i>Peucedani</i>: Extraction, Structural Characterization and Antioxidant Activity |
| title_full | Polysaccharides from Radix <i>Peucedani</i>: Extraction, Structural Characterization and Antioxidant Activity |
| title_fullStr | Polysaccharides from Radix <i>Peucedani</i>: Extraction, Structural Characterization and Antioxidant Activity |
| title_full_unstemmed | Polysaccharides from Radix <i>Peucedani</i>: Extraction, Structural Characterization and Antioxidant Activity |
| title_short | Polysaccharides from Radix <i>Peucedani</i>: Extraction, Structural Characterization and Antioxidant Activity |
| title_sort | polysaccharides from radix i peucedani i extraction structural characterization and antioxidant activity |
| topic | Radix <i>Peucedani</i> polysaccharides deep eutectic solvents network pharmacology molecular docking antioxidant |
| url | https://www.mdpi.com/1420-3049/28/23/7845 |
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