A New Cell Line Derived from the Spleen of the Japanese Flounder (<i>Paralichthys olivaceus</i>) and Its Application in Viral Study
A new cell line Japanese flounder spleen (JFSP) derived from the spleen of Japanese flounder (<i>Paralichthys olivaceus</i>) was established and characterized in this study. The JFSP cells grew rapidly at 29 °C, and the optimum fetal bovine serum concentration in the L-15 medium was 15%....
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/11/12/1697 |
| _version_ | 1797461382114836480 |
|---|---|
| author | Yucong Yang Yuqin Ren Yitong Zhang Guixing Wang Zhongwei He Yufeng Liu Wei Cao Yufen Wang Songlin Chen Yuanshuai Fu Jilun Hou |
| author_facet | Yucong Yang Yuqin Ren Yitong Zhang Guixing Wang Zhongwei He Yufeng Liu Wei Cao Yufen Wang Songlin Chen Yuanshuai Fu Jilun Hou |
| author_sort | Yucong Yang |
| collection | DOAJ |
| description | A new cell line Japanese flounder spleen (JFSP) derived from the spleen of Japanese flounder (<i>Paralichthys olivaceus</i>) was established and characterized in this study. The JFSP cells grew rapidly at 29 °C, and the optimum fetal bovine serum concentration in the L-15 medium was 15%. Cells were subcultured for more than 80 passages. The JFSP cells have a diploid chromosome number of 2n = 68, which differs from the chromosome number of normal diploid Japanese flounder. The established cells were susceptible to Bohle virus (BIV), Viral hemorrhagic septicemia virus (VHSV), Hirame rhabdovirus (HIRRV), Infectious hematopoietic necrosis virus (IHNV), and Lymphocystis disease virus (LCDV), as evidenced by varying degrees of cytopathic effects (CPE). Replication of the virus in JFSP cells was confirmed by qRT-PCR and transmission electron microscopy. In addition, the expression of four immune-related genes, <i>TRAF3</i>, <i>IL-1β</i>, <i>TNF-α</i>, and <i>TLR2</i>, was differentially altered following viral infection. The results indicated that the cells underwent an antiviral immune response. JFSP cell line is an ideal tool <i>in vitro</i> for virology. The use of fish cell lines to study the immune genes and immune mechanism of fish and to clarify the immune mechanism of fish has important theoretical significance and practical application value for the fundamental prevention and treatment of fish diseases. |
| first_indexed | 2024-03-09T17:19:34Z |
| format | Article |
| id | doaj.art-d2da7e923f724ff88c5eea9735082686 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T17:19:34Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-d2da7e923f724ff88c5eea97350826862023-11-24T13:21:49ZengMDPI AGBiology2079-77372022-11-011112169710.3390/biology11121697A New Cell Line Derived from the Spleen of the Japanese Flounder (<i>Paralichthys olivaceus</i>) and Its Application in Viral StudyYucong Yang0Yuqin Ren1Yitong Zhang2Guixing Wang3Zhongwei He4Yufeng Liu5Wei Cao6Yufen Wang7Songlin Chen8Yuanshuai Fu9Jilun Hou10Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, ChinaHebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaHebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaHebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaHebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaHebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaHebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaHebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, ChinaHebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaA new cell line Japanese flounder spleen (JFSP) derived from the spleen of Japanese flounder (<i>Paralichthys olivaceus</i>) was established and characterized in this study. The JFSP cells grew rapidly at 29 °C, and the optimum fetal bovine serum concentration in the L-15 medium was 15%. Cells were subcultured for more than 80 passages. The JFSP cells have a diploid chromosome number of 2n = 68, which differs from the chromosome number of normal diploid Japanese flounder. The established cells were susceptible to Bohle virus (BIV), Viral hemorrhagic septicemia virus (VHSV), Hirame rhabdovirus (HIRRV), Infectious hematopoietic necrosis virus (IHNV), and Lymphocystis disease virus (LCDV), as evidenced by varying degrees of cytopathic effects (CPE). Replication of the virus in JFSP cells was confirmed by qRT-PCR and transmission electron microscopy. In addition, the expression of four immune-related genes, <i>TRAF3</i>, <i>IL-1β</i>, <i>TNF-α</i>, and <i>TLR2</i>, was differentially altered following viral infection. The results indicated that the cells underwent an antiviral immune response. JFSP cell line is an ideal tool <i>in vitro</i> for virology. The use of fish cell lines to study the immune genes and immune mechanism of fish and to clarify the immune mechanism of fish has important theoretical significance and practical application value for the fundamental prevention and treatment of fish diseases.https://www.mdpi.com/2079-7737/11/12/1697<i>Paralichthys olivaceus</i>cell linevirus susceptibilityBohle virusLymphocystis disease viruscell transfection |
| spellingShingle | Yucong Yang Yuqin Ren Yitong Zhang Guixing Wang Zhongwei He Yufeng Liu Wei Cao Yufen Wang Songlin Chen Yuanshuai Fu Jilun Hou A New Cell Line Derived from the Spleen of the Japanese Flounder (<i>Paralichthys olivaceus</i>) and Its Application in Viral Study Biology <i>Paralichthys olivaceus</i> cell line virus susceptibility Bohle virus Lymphocystis disease virus cell transfection |
| title | A New Cell Line Derived from the Spleen of the Japanese Flounder (<i>Paralichthys olivaceus</i>) and Its Application in Viral Study |
| title_full | A New Cell Line Derived from the Spleen of the Japanese Flounder (<i>Paralichthys olivaceus</i>) and Its Application in Viral Study |
| title_fullStr | A New Cell Line Derived from the Spleen of the Japanese Flounder (<i>Paralichthys olivaceus</i>) and Its Application in Viral Study |
| title_full_unstemmed | A New Cell Line Derived from the Spleen of the Japanese Flounder (<i>Paralichthys olivaceus</i>) and Its Application in Viral Study |
| title_short | A New Cell Line Derived from the Spleen of the Japanese Flounder (<i>Paralichthys olivaceus</i>) and Its Application in Viral Study |
| title_sort | new cell line derived from the spleen of the japanese flounder i paralichthys olivaceus i and its application in viral study |
| topic | <i>Paralichthys olivaceus</i> cell line virus susceptibility Bohle virus Lymphocystis disease virus cell transfection |
| url | https://www.mdpi.com/2079-7737/11/12/1697 |
| work_keys_str_mv | AT yucongyang anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yuqinren anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yitongzhang anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT guixingwang anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT zhongweihe anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yufengliu anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT weicao anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yufenwang anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT songlinchen anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yuanshuaifu anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT jilunhou anewcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yucongyang newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yuqinren newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yitongzhang newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT guixingwang newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT zhongweihe newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yufengliu newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT weicao newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yufenwang newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT songlinchen newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT yuanshuaifu newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy AT jilunhou newcelllinederivedfromthespleenofthejapaneseflounderiparalichthysolivaceusianditsapplicationinviralstudy |