The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen <i>Plasmopara viticola</i>
Downy mildew, caused by <i>Plasmopara viticola</i>, is one of the most severe diseases of grapevine (<i>Vitis vinifera</i> L.). Genetic resistance is an effective and sustainable control strategy, but major resistance genes (encoding receptors for specific pathogen effectors)...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/940 |
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| author | Elodie Vandelle Pietro Ariani Alice Regaiolo Davide Danzi Arianna Lovato Claudia Zadra Nicola Vitulo Giorgio Gambino Annalisa Polverari |
| author_facet | Elodie Vandelle Pietro Ariani Alice Regaiolo Davide Danzi Arianna Lovato Claudia Zadra Nicola Vitulo Giorgio Gambino Annalisa Polverari |
| author_sort | Elodie Vandelle |
| collection | DOAJ |
| description | Downy mildew, caused by <i>Plasmopara viticola</i>, is one of the most severe diseases of grapevine (<i>Vitis vinifera</i> L.). Genetic resistance is an effective and sustainable control strategy, but major resistance genes (encoding receptors for specific pathogen effectors) introgressed from wild <i>Vitis</i> species, although effective, may be non-durable because the pathogen can evolve to avoid specific recognition. Previous transcriptomic studies in the resistant species <i>Vitis riparia</i> highlighted the activation of signal transduction components during infection. The transfer of such components to <i>V. vinifera</i> might confer less specific and therefore more durable resistance. Here, we describe the generation of transgenic <i>V. vinifera</i> lines constitutively expressing the <i>V. riparia</i> E3 ubiquitin ligase gene <i>VriATL156</i>. Phenotypic and molecular analysis revealed that the transgenic plants were less susceptible to <i>P. viticola</i> than vector-only controls, confirming the role of this E3 ubiquitin ligase in the innate immune response. Two independent transgenic lines were selected for detailed analysis of the resistance phenotype by RNA-Seq and microscopy, revealing the profound reprogramming of transcription to achieve resistance that operates from the earliest stages of pathogen infection. The introduction of <i>VriATL156</i> into elite grapevine cultivars could therefore provide an effective and sustainable control measure against downy mildew. |
| first_indexed | 2024-03-09T04:21:21Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:21:21Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d2df7da4ab794655ab79730b08f533122023-12-03T13:47:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122294010.3390/ijms22020940The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen <i>Plasmopara viticola</i>Elodie Vandelle0Pietro Ariani1Alice Regaiolo2Davide Danzi3Arianna Lovato4Claudia Zadra5Nicola Vitulo6Giorgio Gambino7Annalisa Polverari8Department of Biotechnology, University of Verona, Strada Le Grazie 15, CV1, 37134 Verona, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie 15, CV1, 37134 Verona, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie 15, CV1, 37134 Verona, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie 15, CV1, 37134 Verona, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie 15, CV1, 37134 Verona, ItalyDepartment of Pharmaceutical Sciences, University of Perugia, Borgo XX Giugno 72, 06121 Perugia, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie 15, CV1, 37134 Verona, ItalyInstitute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie 15, CV1, 37134 Verona, ItalyDowny mildew, caused by <i>Plasmopara viticola</i>, is one of the most severe diseases of grapevine (<i>Vitis vinifera</i> L.). Genetic resistance is an effective and sustainable control strategy, but major resistance genes (encoding receptors for specific pathogen effectors) introgressed from wild <i>Vitis</i> species, although effective, may be non-durable because the pathogen can evolve to avoid specific recognition. Previous transcriptomic studies in the resistant species <i>Vitis riparia</i> highlighted the activation of signal transduction components during infection. The transfer of such components to <i>V. vinifera</i> might confer less specific and therefore more durable resistance. Here, we describe the generation of transgenic <i>V. vinifera</i> lines constitutively expressing the <i>V. riparia</i> E3 ubiquitin ligase gene <i>VriATL156</i>. Phenotypic and molecular analysis revealed that the transgenic plants were less susceptible to <i>P. viticola</i> than vector-only controls, confirming the role of this E3 ubiquitin ligase in the innate immune response. Two independent transgenic lines were selected for detailed analysis of the resistance phenotype by RNA-Seq and microscopy, revealing the profound reprogramming of transcription to achieve resistance that operates from the earliest stages of pathogen infection. The introduction of <i>VriATL156</i> into elite grapevine cultivars could therefore provide an effective and sustainable control measure against downy mildew.https://www.mdpi.com/1422-0067/22/2/940ATL E3 ubiquitin ligasesplant functional genomicspromoter analysisplant resistancesustainable agriculture |
| spellingShingle | Elodie Vandelle Pietro Ariani Alice Regaiolo Davide Danzi Arianna Lovato Claudia Zadra Nicola Vitulo Giorgio Gambino Annalisa Polverari The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen <i>Plasmopara viticola</i> International Journal of Molecular Sciences ATL E3 ubiquitin ligases plant functional genomics promoter analysis plant resistance sustainable agriculture |
| title | The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen <i>Plasmopara viticola</i> |
| title_full | The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen <i>Plasmopara viticola</i> |
| title_fullStr | The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen <i>Plasmopara viticola</i> |
| title_full_unstemmed | The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen <i>Plasmopara viticola</i> |
| title_short | The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen <i>Plasmopara viticola</i> |
| title_sort | grapevine e3 ubiquitin ligase vriatl156 confers resistance against the downy mildew pathogen i plasmopara viticola i |
| topic | ATL E3 ubiquitin ligases plant functional genomics promoter analysis plant resistance sustainable agriculture |
| url | https://www.mdpi.com/1422-0067/22/2/940 |
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