Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation
Understanding the different regulatory functions of epithelial and mesenchymal cell types in salivary gland development and cellular organization is essential for proper organoid formation and salivary gland tissue regeneration. Here, we demonstrate a biocompatible platform using pre-formed alginate...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/9/1/38 |
| _version_ | 1827666697917562880 |
|---|---|
| author | Matthew Jorgensen Pujhitha Ramesh Miriam Toro Emily Evans Nicholas Moskwa Xulang Zhang Susan T. Sharfstein Melinda Larsen Yubing Xie |
| author_facet | Matthew Jorgensen Pujhitha Ramesh Miriam Toro Emily Evans Nicholas Moskwa Xulang Zhang Susan T. Sharfstein Melinda Larsen Yubing Xie |
| author_sort | Matthew Jorgensen |
| collection | DOAJ |
| description | Understanding the different regulatory functions of epithelial and mesenchymal cell types in salivary gland development and cellular organization is essential for proper organoid formation and salivary gland tissue regeneration. Here, we demonstrate a biocompatible platform using pre-formed alginate hydrogel microtubes to facilitate direct epithelial–mesenchymal cell interaction for 3D salivary gland cell organization, which allows for monitoring cellular organization while providing a protective barrier from cell-cluster loss during medium changes. Using mouse salivary gland ductal epithelial SIMS cells as the epithelial model cell type and NIH 3T3 fibroblasts or primary E16 salivary mesenchyme cells as the stromal model cell types, self-organization from epithelial–mesenchymal interaction was examined. We observed that epithelial and mesenchymal cells undergo aggregation on day 1, cavitation by day 4, and generation of an EpCAM-expressing epithelial cell layer as early as day 7 of the co-culture in hydrogel microtubes, demonstrating the utility of hydrogel microtubes to facilitate heterotypic cell–cell interactions to form cavitated organoids. Thus, pre-formed alginate microtubes are a promising co-culture method for further understanding epithelial and mesenchymal interaction during tissue morphogenesis and for future practical applications in regenerative medicine. |
| first_indexed | 2024-03-10T01:55:22Z |
| format | Article |
| id | doaj.art-d2e6efc7c64c4826881ee6d2baed7ca4 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T01:55:22Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-d2e6efc7c64c4826881ee6d2baed7ca42023-11-23T12:59:27ZengMDPI AGBioengineering2306-53542022-01-01913810.3390/bioengineering9010038Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and CavitationMatthew Jorgensen0Pujhitha Ramesh1Miriam Toro2Emily Evans3Nicholas Moskwa4Xulang Zhang5Susan T. Sharfstein6Melinda Larsen7Yubing Xie8College of Nanoscale Science and Engineering, SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY 12203, USACollege of Nanoscale Science and Engineering, SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY 12203, USACollege of Nanoscale Science and Engineering, SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY 12203, USACollege of Nanoscale Science and Engineering, SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY 12203, USADepartment of Biological Sciences, State University of New York, University at Albany, 1400 Washington Ave., Albany, NY 12222, USACollege of Nanoscale Science and Engineering, SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY 12203, USACollege of Nanoscale Science and Engineering, SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY 12203, USADepartment of Biological Sciences, State University of New York, University at Albany, 1400 Washington Ave., Albany, NY 12222, USACollege of Nanoscale Science and Engineering, SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY 12203, USAUnderstanding the different regulatory functions of epithelial and mesenchymal cell types in salivary gland development and cellular organization is essential for proper organoid formation and salivary gland tissue regeneration. Here, we demonstrate a biocompatible platform using pre-formed alginate hydrogel microtubes to facilitate direct epithelial–mesenchymal cell interaction for 3D salivary gland cell organization, which allows for monitoring cellular organization while providing a protective barrier from cell-cluster loss during medium changes. Using mouse salivary gland ductal epithelial SIMS cells as the epithelial model cell type and NIH 3T3 fibroblasts or primary E16 salivary mesenchyme cells as the stromal model cell types, self-organization from epithelial–mesenchymal interaction was examined. We observed that epithelial and mesenchymal cells undergo aggregation on day 1, cavitation by day 4, and generation of an EpCAM-expressing epithelial cell layer as early as day 7 of the co-culture in hydrogel microtubes, demonstrating the utility of hydrogel microtubes to facilitate heterotypic cell–cell interactions to form cavitated organoids. Thus, pre-formed alginate microtubes are a promising co-culture method for further understanding epithelial and mesenchymal interaction during tissue morphogenesis and for future practical applications in regenerative medicine.https://www.mdpi.com/2306-5354/9/1/38alginatehydrogelmicrotube3D culturesalivary glandorganoids |
| spellingShingle | Matthew Jorgensen Pujhitha Ramesh Miriam Toro Emily Evans Nicholas Moskwa Xulang Zhang Susan T. Sharfstein Melinda Larsen Yubing Xie Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation Bioengineering alginate hydrogel microtube 3D culture salivary gland organoids |
| title | Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation |
| title_full | Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation |
| title_fullStr | Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation |
| title_full_unstemmed | Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation |
| title_short | Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation |
| title_sort | alginate hydrogel microtubes for salivary gland cell organization and cavitation |
| topic | alginate hydrogel microtube 3D culture salivary gland organoids |
| url | https://www.mdpi.com/2306-5354/9/1/38 |
| work_keys_str_mv | AT matthewjorgensen alginatehydrogelmicrotubesforsalivaryglandcellorganizationandcavitation AT pujhitharamesh alginatehydrogelmicrotubesforsalivaryglandcellorganizationandcavitation AT miriamtoro alginatehydrogelmicrotubesforsalivaryglandcellorganizationandcavitation AT emilyevans alginatehydrogelmicrotubesforsalivaryglandcellorganizationandcavitation AT nicholasmoskwa alginatehydrogelmicrotubesforsalivaryglandcellorganizationandcavitation AT xulangzhang alginatehydrogelmicrotubesforsalivaryglandcellorganizationandcavitation AT susantsharfstein alginatehydrogelmicrotubesforsalivaryglandcellorganizationandcavitation AT melindalarsen alginatehydrogelmicrotubesforsalivaryglandcellorganizationandcavitation AT yubingxie alginatehydrogelmicrotubesforsalivaryglandcellorganizationandcavitation |