Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows
Endometritis is a disease that affects reproductive health in dairy cows and causes serious economic damage to the dairy industry world-wide. Although in recent years, the application of mesenchymal stem cell (MSC) therapy for the treatment of inflammatory diseases has attracted much attention, ther...
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| Format: | Article |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.726328/full |
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| author | Wengeng Lu Wengeng Lu Zheng-Mei Xu Qing Liu Nan-Nan Yu Jia-Bin Yu Wei-Long Li Ying-Ying Mao Zhenzhen Du Linqing Si Siqi Yuan Jidong Jin Shixin Fu Shixin Fu Dongbo Sun Ying-Hao Han |
| author_facet | Wengeng Lu Wengeng Lu Zheng-Mei Xu Qing Liu Nan-Nan Yu Jia-Bin Yu Wei-Long Li Ying-Ying Mao Zhenzhen Du Linqing Si Siqi Yuan Jidong Jin Shixin Fu Shixin Fu Dongbo Sun Ying-Hao Han |
| author_sort | Wengeng Lu |
| collection | DOAJ |
| description | Endometritis is a disease that affects reproductive health in dairy cows and causes serious economic damage to the dairy industry world-wide. Although in recent years, the application of mesenchymal stem cell (MSC) therapy for the treatment of inflammatory diseases has attracted much attention, there are few reports of the use of MSCs in dairy cows. In the present study, our objective was to explore the inhibitory effects of bovine adipose-derived mesenchymal stem cells (bAD-MSCs) on lipopolysaccharide (LPS) induced inflammation in bovine endometrial epithelial cells (bEECs) along with the potential underlying molecular mechanisms. We characterized isolated bAD-MSCs using cell surface marker staining and adipogenic/osteogenic differentiation, and analyzed them using immunofluorescence, flow cytometry (surface marker staining), and adipogenic and osteogenic differentiation. Furthermore, to understand the anti-inflammatory effects of bAD-MSCs on LPS induced bEEC inflammation, we used a bAD-MSC/bEEC co-culture system. The results showed that bAD-MSC treatments could significantly decrease LPS induced bEEC apoptosis and pro-inflammatory cytokine expression levels, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α). Furthermore, our results showed that bAD-MSC treatments could also significantly downregulate LPS induced p38, IkB-a, and JAK1 phosphorylation and Bax protein expression levels, which are closely related to inflammatory progress and cellular apoptosis in bEECs. Our findings demonstrate that bAD-MSCs play an inhibitory role in LPS induced bEEC inflammation and provide new insights for the clinical therapy of endometritis in dairy cows. |
| first_indexed | 2024-12-16T07:21:01Z |
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| id | doaj.art-d2e99d675b8e486e905b5412d618027e |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-16T07:21:01Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-d2e99d675b8e486e905b5412d618027e2022-12-21T22:39:39ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-10-01810.3389/fvets.2021.726328726328Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy CowsWengeng Lu0Wengeng Lu1Zheng-Mei Xu2Qing Liu3Nan-Nan Yu4Jia-Bin Yu5Wei-Long Li6Ying-Ying Mao7Zhenzhen Du8Linqing Si9Siqi Yuan10Jidong Jin11Shixin Fu12Shixin Fu13Dongbo Sun14Ying-Hao Han15Laboratory of Theriogenology and Reproductive Health, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaHeilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaLaboratory of Theriogenology and Reproductive Health, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaLaboratory of Theriogenology and Reproductive Health, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaLaboratory of Stem Cell Therapy and Regenration Biology, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, ChinaLaboratory of Stem Cell Therapy and Regenration Biology, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, ChinaLaboratory of Stem Cell Therapy and Regenration Biology, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, ChinaLaboratory of Stem Cell Therapy and Regenration Biology, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, ChinaLaboratory of Theriogenology and Reproductive Health, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaLaboratory of Theriogenology and Reproductive Health, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaLaboratory of Theriogenology and Reproductive Health, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaCofeed Feedmill (Changchun) Co., Ltd., Changchun, ChinaLaboratory of Theriogenology and Reproductive Health, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaHeilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaHeilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaLaboratory of Stem Cell Therapy and Regenration Biology, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, ChinaEndometritis is a disease that affects reproductive health in dairy cows and causes serious economic damage to the dairy industry world-wide. Although in recent years, the application of mesenchymal stem cell (MSC) therapy for the treatment of inflammatory diseases has attracted much attention, there are few reports of the use of MSCs in dairy cows. In the present study, our objective was to explore the inhibitory effects of bovine adipose-derived mesenchymal stem cells (bAD-MSCs) on lipopolysaccharide (LPS) induced inflammation in bovine endometrial epithelial cells (bEECs) along with the potential underlying molecular mechanisms. We characterized isolated bAD-MSCs using cell surface marker staining and adipogenic/osteogenic differentiation, and analyzed them using immunofluorescence, flow cytometry (surface marker staining), and adipogenic and osteogenic differentiation. Furthermore, to understand the anti-inflammatory effects of bAD-MSCs on LPS induced bEEC inflammation, we used a bAD-MSC/bEEC co-culture system. The results showed that bAD-MSC treatments could significantly decrease LPS induced bEEC apoptosis and pro-inflammatory cytokine expression levels, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α). Furthermore, our results showed that bAD-MSC treatments could also significantly downregulate LPS induced p38, IkB-a, and JAK1 phosphorylation and Bax protein expression levels, which are closely related to inflammatory progress and cellular apoptosis in bEECs. Our findings demonstrate that bAD-MSCs play an inhibitory role in LPS induced bEEC inflammation and provide new insights for the clinical therapy of endometritis in dairy cows.https://www.frontiersin.org/articles/10.3389/fvets.2021.726328/fullbovine adipose-derived mesenchymal stem cellinflammationlipopolysaccharideendometritisdairy cows |
| spellingShingle | Wengeng Lu Wengeng Lu Zheng-Mei Xu Qing Liu Nan-Nan Yu Jia-Bin Yu Wei-Long Li Ying-Ying Mao Zhenzhen Du Linqing Si Siqi Yuan Jidong Jin Shixin Fu Shixin Fu Dongbo Sun Ying-Hao Han Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows Frontiers in Veterinary Science bovine adipose-derived mesenchymal stem cell inflammation lipopolysaccharide endometritis dairy cows |
| title | Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows |
| title_full | Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows |
| title_fullStr | Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows |
| title_full_unstemmed | Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows |
| title_short | Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows |
| title_sort | inhibitory effect of bovine adipose derived mesenchymal stem cells on lipopolysaccharide induced inflammation of endometrial epithelial cells in dairy cows |
| topic | bovine adipose-derived mesenchymal stem cell inflammation lipopolysaccharide endometritis dairy cows |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.726328/full |
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