Current Trends in the Use of Semiconducting Materials for Electrochemical Aptasensing
Aptamers are synthetic single-stranded oligonucleotides that exhibit selective binding properties to specific targets, thereby providing a powerful basis for the development of selective and sensitive (bio)chemical assays. Electrochemical biosensors utilizing aptamers as biological recognition eleme...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/11/8/438 |
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| author | Leda Bousiakou Omar Al-Dosary Anastasios Economou Veronika Subjakova Tibor Hianik |
| author_facet | Leda Bousiakou Omar Al-Dosary Anastasios Economou Veronika Subjakova Tibor Hianik |
| author_sort | Leda Bousiakou |
| collection | DOAJ |
| description | Aptamers are synthetic single-stranded oligonucleotides that exhibit selective binding properties to specific targets, thereby providing a powerful basis for the development of selective and sensitive (bio)chemical assays. Electrochemical biosensors utilizing aptamers as biological recognition elements, namely aptasensors, are at the forefront of current research. They exploit the combination of the unique properties of aptamers with the advantages of electrochemical detection with the view to fabricate inexpensive and portable analytical platforms for rapid detection in point-of-care (POC) applications or for on-site monitoring. The immobilization of aptamers on suitable substrates is of paramount importance in order to preserve their functionality and optimize the sensors’ sensitivity. This work describes different immobilization strategies for aptamers on the surface of semiconductor-based working electrodes, including metal oxides, conductive polymers, and carbon allotropes. These are presented as platforms with tunable band gaps and various surface morphologies for the preparation of low cost, highly versatile aptasensor devices in analytical chemistry. A survey of the current literature is provided, discussing each analytical method. Future trends are outlined which envisage aptamer-based biosensing using semiconductors. |
| first_indexed | 2024-03-11T00:02:46Z |
| format | Article |
| id | doaj.art-d2f18d0abc134fbd9dd458466474e19a |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-11T00:02:46Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-d2f18d0abc134fbd9dd458466474e19a2023-11-19T00:38:28ZengMDPI AGChemosensors2227-90402023-08-0111843810.3390/chemosensors11080438Current Trends in the Use of Semiconducting Materials for Electrochemical AptasensingLeda Bousiakou0Omar Al-Dosary1Anastasios Economou2Veronika Subjakova3Tibor Hianik4IMD Laboratories Co., R&D Section, Lefkippos Technology Park, National Centre for Scientific Research (NCSR) Demokritos, Agia Paraskevi, 15130 Athens, GreeceDepartment of Physics and Astronomy, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Chemistry, National and Kapodistrian University, 15771 Athens, GreeceDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, 842 48 Bratislava, SlovakiaDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, 842 48 Bratislava, SlovakiaAptamers are synthetic single-stranded oligonucleotides that exhibit selective binding properties to specific targets, thereby providing a powerful basis for the development of selective and sensitive (bio)chemical assays. Electrochemical biosensors utilizing aptamers as biological recognition elements, namely aptasensors, are at the forefront of current research. They exploit the combination of the unique properties of aptamers with the advantages of electrochemical detection with the view to fabricate inexpensive and portable analytical platforms for rapid detection in point-of-care (POC) applications or for on-site monitoring. The immobilization of aptamers on suitable substrates is of paramount importance in order to preserve their functionality and optimize the sensors’ sensitivity. This work describes different immobilization strategies for aptamers on the surface of semiconductor-based working electrodes, including metal oxides, conductive polymers, and carbon allotropes. These are presented as platforms with tunable band gaps and various surface morphologies for the preparation of low cost, highly versatile aptasensor devices in analytical chemistry. A survey of the current literature is provided, discussing each analytical method. Future trends are outlined which envisage aptamer-based biosensing using semiconductors.https://www.mdpi.com/2227-9040/11/8/438DNA aptamerselectrochemical sensorssemiconductorsnanomaterialscarbon materialsmetal oxides |
| spellingShingle | Leda Bousiakou Omar Al-Dosary Anastasios Economou Veronika Subjakova Tibor Hianik Current Trends in the Use of Semiconducting Materials for Electrochemical Aptasensing Chemosensors DNA aptamers electrochemical sensors semiconductors nanomaterials carbon materials metal oxides |
| title | Current Trends in the Use of Semiconducting Materials for Electrochemical Aptasensing |
| title_full | Current Trends in the Use of Semiconducting Materials for Electrochemical Aptasensing |
| title_fullStr | Current Trends in the Use of Semiconducting Materials for Electrochemical Aptasensing |
| title_full_unstemmed | Current Trends in the Use of Semiconducting Materials for Electrochemical Aptasensing |
| title_short | Current Trends in the Use of Semiconducting Materials for Electrochemical Aptasensing |
| title_sort | current trends in the use of semiconducting materials for electrochemical aptasensing |
| topic | DNA aptamers electrochemical sensors semiconductors nanomaterials carbon materials metal oxides |
| url | https://www.mdpi.com/2227-9040/11/8/438 |
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