Biosurfactant Production by <i>Bacillus amyloliquefaciens</i> C11 and <i>Streptomyces lavendulae</i> C27 Isolated from a Biopurification System for Environmental Applications
Biosurfactant-producing bacteria can be found in contaminated environments such as biopurification systems (BPS) for pesticide treatments. A total of 18 isolates were screened to determine their ability to produce extracellular biosurfactants, using olive oil as the main carbon source. Out of the ei...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/10/1892 |
| _version_ | 1797471199064752128 |
|---|---|
| author | M. Cristina Diez Cesar Llafquen Paola Fincheira Claudio Lamilla Gabriela Briceño Heidi Schalchli |
| author_facet | M. Cristina Diez Cesar Llafquen Paola Fincheira Claudio Lamilla Gabriela Briceño Heidi Schalchli |
| author_sort | M. Cristina Diez |
| collection | DOAJ |
| description | Biosurfactant-producing bacteria can be found in contaminated environments such as biopurification systems (BPS) for pesticide treatments. A total of 18 isolates were screened to determine their ability to produce extracellular biosurfactants, using olive oil as the main carbon source. Out of the eighteen isolates, two strains (C11 and C27) were selected for biosurfactant production. The emulsification activities of the C11 and C27 strains using sunflower oil was 58.4 and 53.7%, respectively, and 46.6 and 48.0% using olive oil. Using molecular techniques and MALDI-TOF, the strains were identified as <i>Bacillus amyloliquefaciens</i> (C11) and <i>Streptomyces lavendulae</i> (C27). The submerged cultivation of the two selected strains was carried out in a 1 L stirred-tank bioreactor. The maximum biosurfactant production, indicated by the lowest surface tension measurement, was similar (46 and 45 mN/m) for both strains, independent of the fact that the biomass of the <i>B. amyloliquefaciens</i> C11 strain was 50% lower than the biomass of the <i>S. lavendulae</i> C27 strain. The partially purified biosurfactants produced by <i>B. amyloliquefaciens</i> C11 and <i>S. lavendulae</i> C27 were characterized as a lipopeptide and a glycolipid, respectively. These outcomes highlight the potential of the selected biosurfactant-producing microorganisms for improving pesticides’ bioavailability and therefore the degradational efficacy of BPS. |
| first_indexed | 2024-03-09T19:45:46Z |
| format | Article |
| id | doaj.art-d2f56547fd85449089dbc229e4de2de5 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T19:45:46Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d2f56547fd85449089dbc229e4de2de52023-11-24T01:24:58ZengMDPI AGMicroorganisms2076-26072022-09-011010189210.3390/microorganisms10101892Biosurfactant Production by <i>Bacillus amyloliquefaciens</i> C11 and <i>Streptomyces lavendulae</i> C27 Isolated from a Biopurification System for Environmental ApplicationsM. Cristina Diez0Cesar Llafquen1Paola Fincheira2Claudio Lamilla3Gabriela Briceño4Heidi Schalchli5Chemical Engineering Department, University of La Frontera, Av. Francisco Salazar 01145, Temuco 4780000, ChileBiotechnological Research Center Applied to the Environment (CIBAMA-BIOREN), University of La Frontera, Av. Francisco Salazar 01145, Temuco 4780000, ChileBiotechnological Research Center Applied to the Environment (CIBAMA-BIOREN), University of La Frontera, Av. Francisco Salazar 01145, Temuco 4780000, ChileBiotechnological Research Center Applied to the Environment (CIBAMA-BIOREN), University of La Frontera, Av. Francisco Salazar 01145, Temuco 4780000, ChileBiotechnological Research Center Applied to the Environment (CIBAMA-BIOREN), University of La Frontera, Av. Francisco Salazar 01145, Temuco 4780000, ChileBiotechnological Research Center Applied to the Environment (CIBAMA-BIOREN), University of La Frontera, Av. Francisco Salazar 01145, Temuco 4780000, ChileBiosurfactant-producing bacteria can be found in contaminated environments such as biopurification systems (BPS) for pesticide treatments. A total of 18 isolates were screened to determine their ability to produce extracellular biosurfactants, using olive oil as the main carbon source. Out of the eighteen isolates, two strains (C11 and C27) were selected for biosurfactant production. The emulsification activities of the C11 and C27 strains using sunflower oil was 58.4 and 53.7%, respectively, and 46.6 and 48.0% using olive oil. Using molecular techniques and MALDI-TOF, the strains were identified as <i>Bacillus amyloliquefaciens</i> (C11) and <i>Streptomyces lavendulae</i> (C27). The submerged cultivation of the two selected strains was carried out in a 1 L stirred-tank bioreactor. The maximum biosurfactant production, indicated by the lowest surface tension measurement, was similar (46 and 45 mN/m) for both strains, independent of the fact that the biomass of the <i>B. amyloliquefaciens</i> C11 strain was 50% lower than the biomass of the <i>S. lavendulae</i> C27 strain. The partially purified biosurfactants produced by <i>B. amyloliquefaciens</i> C11 and <i>S. lavendulae</i> C27 were characterized as a lipopeptide and a glycolipid, respectively. These outcomes highlight the potential of the selected biosurfactant-producing microorganisms for improving pesticides’ bioavailability and therefore the degradational efficacy of BPS.https://www.mdpi.com/2076-2607/10/10/1892biosurfactants<i>Bacillus</i>lipopeptide<i>Streptomyces</i>glycolipidsenvironmental applications |
| spellingShingle | M. Cristina Diez Cesar Llafquen Paola Fincheira Claudio Lamilla Gabriela Briceño Heidi Schalchli Biosurfactant Production by <i>Bacillus amyloliquefaciens</i> C11 and <i>Streptomyces lavendulae</i> C27 Isolated from a Biopurification System for Environmental Applications Microorganisms biosurfactants <i>Bacillus</i> lipopeptide <i>Streptomyces</i> glycolipids environmental applications |
| title | Biosurfactant Production by <i>Bacillus amyloliquefaciens</i> C11 and <i>Streptomyces lavendulae</i> C27 Isolated from a Biopurification System for Environmental Applications |
| title_full | Biosurfactant Production by <i>Bacillus amyloliquefaciens</i> C11 and <i>Streptomyces lavendulae</i> C27 Isolated from a Biopurification System for Environmental Applications |
| title_fullStr | Biosurfactant Production by <i>Bacillus amyloliquefaciens</i> C11 and <i>Streptomyces lavendulae</i> C27 Isolated from a Biopurification System for Environmental Applications |
| title_full_unstemmed | Biosurfactant Production by <i>Bacillus amyloliquefaciens</i> C11 and <i>Streptomyces lavendulae</i> C27 Isolated from a Biopurification System for Environmental Applications |
| title_short | Biosurfactant Production by <i>Bacillus amyloliquefaciens</i> C11 and <i>Streptomyces lavendulae</i> C27 Isolated from a Biopurification System for Environmental Applications |
| title_sort | biosurfactant production by i bacillus amyloliquefaciens i c11 and i streptomyces lavendulae i c27 isolated from a biopurification system for environmental applications |
| topic | biosurfactants <i>Bacillus</i> lipopeptide <i>Streptomyces</i> glycolipids environmental applications |
| url | https://www.mdpi.com/2076-2607/10/10/1892 |
| work_keys_str_mv | AT mcristinadiez biosurfactantproductionbyibacillusamyloliquefaciensic11andistreptomyceslavendulaeic27isolatedfromabiopurificationsystemforenvironmentalapplications AT cesarllafquen biosurfactantproductionbyibacillusamyloliquefaciensic11andistreptomyceslavendulaeic27isolatedfromabiopurificationsystemforenvironmentalapplications AT paolafincheira biosurfactantproductionbyibacillusamyloliquefaciensic11andistreptomyceslavendulaeic27isolatedfromabiopurificationsystemforenvironmentalapplications AT claudiolamilla biosurfactantproductionbyibacillusamyloliquefaciensic11andistreptomyceslavendulaeic27isolatedfromabiopurificationsystemforenvironmentalapplications AT gabrielabriceno biosurfactantproductionbyibacillusamyloliquefaciensic11andistreptomyceslavendulaeic27isolatedfromabiopurificationsystemforenvironmentalapplications AT heidischalchli biosurfactantproductionbyibacillusamyloliquefaciensic11andistreptomyceslavendulaeic27isolatedfromabiopurificationsystemforenvironmentalapplications |