Pressure-assisted cold denaturation of hen egg white lysozyme: the influence of co-solvents probed by hydrogen exchange nuclear magnetic resonance

COSY proton nuclear magnetic resonance was used to measure the exchange rates of amide protons of hen egg white lysozyme (HEWL) in the pressure-assisted cold-denatured state and in the heat-denatured state. After dissolving lysozyme in deuterium oxide buffer, labile protons exchange for deuterons in...

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Detalhes bibliográficos
Principais autores: K. Vogtt, R. Winter
Formato: Artigo
Idioma:English
Publicado em: Associação Brasileira de Divulgação Científica 2005-08-01
coleção:Brazilian Journal of Medical and Biological Research
Assuntos:
Acesso em linha:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800005