Pressure-assisted cold denaturation of hen egg white lysozyme: the influence of co-solvents probed by hydrogen exchange nuclear magnetic resonance
COSY proton nuclear magnetic resonance was used to measure the exchange rates of amide protons of hen egg white lysozyme (HEWL) in the pressure-assisted cold-denatured state and in the heat-denatured state. After dissolving lysozyme in deuterium oxide buffer, labile protons exchange for deuterons in...
Principais autores: | , |
---|---|
Formato: | Artigo |
Idioma: | English |
Publicado em: |
Associação Brasileira de Divulgação Científica
2005-08-01
|
coleção: | Brazilian Journal of Medical and Biological Research |
Assuntos: | |
Acesso em linha: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800005 |