Suppression of neuronal apoptosis and glial activation with modulation of Nrf2/HO-1 and NF-kB signaling by curcumin in streptozotocin-induced diabetic spinal cord central neuropathy
IntroductionDiabetes is a global disease, commonly complicated by neuropathy. The spinal cord reacts to diabetes by neuronal apoptosis, microglial activation, and astrocytosis, with a disturbance in neuronal and glial Nuclear factor erythroid 2-related factor/Heme oxygenase-1 (Nrf2/HO-1) and Nuclear...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnana.2023.1094301/full |
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| author | Hassan Reda Hassan Elsayed Hassan Reda Hassan Elsayed Mohammed R. Rabei Mohammed R. Rabei Mohamed Mahmoud Abdelraheem Elshaer Mohamed Mahmoud Abdelraheem Elshaer Eman Mohamad El Nashar Eman Mohamad El Nashar Mansour Abdullah Alghamdi Mansour Abdullah Alghamdi Zainah Al-Qahtani Ahmed Nabawy Ahmed Nabawy |
| author_facet | Hassan Reda Hassan Elsayed Hassan Reda Hassan Elsayed Mohammed R. Rabei Mohammed R. Rabei Mohamed Mahmoud Abdelraheem Elshaer Mohamed Mahmoud Abdelraheem Elshaer Eman Mohamad El Nashar Eman Mohamad El Nashar Mansour Abdullah Alghamdi Mansour Abdullah Alghamdi Zainah Al-Qahtani Ahmed Nabawy Ahmed Nabawy |
| author_sort | Hassan Reda Hassan Elsayed |
| collection | DOAJ |
| description | IntroductionDiabetes is a global disease, commonly complicated by neuropathy. The spinal cord reacts to diabetes by neuronal apoptosis, microglial activation, and astrocytosis, with a disturbance in neuronal and glial Nuclear factor erythroid 2-related factor/Heme oxygenase-1 (Nrf2/HO-1) and Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signaling. Curcumin, a bioactive natural substance, showed neuroprotective role in many diseases. However, its role in the treatment of the diabetic central neuropathy of spinal cord and the underlying mechanisms still need clarification. The present study tried to evaluate the role of curcumin in diabetes-induced central neuropathy of the spinal cord in rats.MethodsTwenty rats were divided into three groups; group 1: a negative control group; group 2: received streptozotocin (STZ) to induce type I diabetes, and group 3: received STZ + Curcumin (150 mg/kg/day) for eight weeks. The spinal cords were examined for histopathological changes, and immunohistochemical staining for Glia fibrillary acidic protein (GFAP); an astrocyte marker, Ionized calcium-binding adaptor molecule 1 (Iba1), a microglial marker, neuronal nuclear protein (NeuN); a neuronal marker, caspase-3; an apoptosis marker, Nrf2/HO-1, NF-kB, and oxidative stress markers were assessed.ResultsCurcumin could improve spinal cord changes, suppress the expression of Iba1, GFAP, caspase-3, and NF-kB, and could increase the expression of NeuN and restore the Nrf2/HO-1 signaling.DiscussionCurcumin could suppress diabetic spinal cord central neuropathy, glial activation, and neuronal apoptosis with the regulation of Nrf2/HO-1 and NF-kB signaling. |
| first_indexed | 2024-04-10T04:37:56Z |
| format | Article |
| id | doaj.art-d2fac8e099534bc8abfb4b611720cdf8 |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-04-10T04:37:56Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-d2fac8e099534bc8abfb4b611720cdf82023-03-09T16:55:54ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292023-03-011710.3389/fnana.2023.10943011094301Suppression of neuronal apoptosis and glial activation with modulation of Nrf2/HO-1 and NF-kB signaling by curcumin in streptozotocin-induced diabetic spinal cord central neuropathyHassan Reda Hassan Elsayed0Hassan Reda Hassan Elsayed1Mohammed R. Rabei2Mohammed R. Rabei3Mohamed Mahmoud Abdelraheem Elshaer4Mohamed Mahmoud Abdelraheem Elshaer5Eman Mohamad El Nashar6Eman Mohamad El Nashar7Mansour Abdullah Alghamdi8Mansour Abdullah Alghamdi9Zainah Al-Qahtani10Ahmed Nabawy11Ahmed Nabawy12Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura, EgyptDepartment of Anatomy, Faculty of Medicine, New Mansoura University, New Mansoura City, EgyptDepartment of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura, EgyptDepartment of Physiology, Faculty of Medicine, King Salman International University, South Sinai, EgyptDepartment of Clinical Pharmacology, Faculty of Medicine, Ain Shams University, Cairo, EgyptDepartment of Clinical Pharmacology, Faculty of Medicine, King Salman International University, South Sinai, EgyptDepartment of Anatomy, College Medicine, King Khalid University, Abha, Saudi ArabiaDepartment of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha, EgyptDepartment of Anatomy, College Medicine, King Khalid University, Abha, Saudi ArabiaGenomics and Personalized Medicine Unit, College of Medicine, King Khalid University, Abha, Saudi Arabia0Neurology Section, Internal Medicine Department, College of Medicine, King Khalid University, Abha, Saudi ArabiaDepartment of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura, EgyptDepartment of Anatomy, Faculty of Medicine, New Mansoura University, New Mansoura City, EgyptIntroductionDiabetes is a global disease, commonly complicated by neuropathy. The spinal cord reacts to diabetes by neuronal apoptosis, microglial activation, and astrocytosis, with a disturbance in neuronal and glial Nuclear factor erythroid 2-related factor/Heme oxygenase-1 (Nrf2/HO-1) and Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signaling. Curcumin, a bioactive natural substance, showed neuroprotective role in many diseases. However, its role in the treatment of the diabetic central neuropathy of spinal cord and the underlying mechanisms still need clarification. The present study tried to evaluate the role of curcumin in diabetes-induced central neuropathy of the spinal cord in rats.MethodsTwenty rats were divided into three groups; group 1: a negative control group; group 2: received streptozotocin (STZ) to induce type I diabetes, and group 3: received STZ + Curcumin (150 mg/kg/day) for eight weeks. The spinal cords were examined for histopathological changes, and immunohistochemical staining for Glia fibrillary acidic protein (GFAP); an astrocyte marker, Ionized calcium-binding adaptor molecule 1 (Iba1), a microglial marker, neuronal nuclear protein (NeuN); a neuronal marker, caspase-3; an apoptosis marker, Nrf2/HO-1, NF-kB, and oxidative stress markers were assessed.ResultsCurcumin could improve spinal cord changes, suppress the expression of Iba1, GFAP, caspase-3, and NF-kB, and could increase the expression of NeuN and restore the Nrf2/HO-1 signaling.DiscussionCurcumin could suppress diabetic spinal cord central neuropathy, glial activation, and neuronal apoptosis with the regulation of Nrf2/HO-1 and NF-kB signaling.https://www.frontiersin.org/articles/10.3389/fnana.2023.1094301/fulldiabetic neuropathymicrogliaastrocytescurcuminNrf2HO-1 |
| spellingShingle | Hassan Reda Hassan Elsayed Hassan Reda Hassan Elsayed Mohammed R. Rabei Mohammed R. Rabei Mohamed Mahmoud Abdelraheem Elshaer Mohamed Mahmoud Abdelraheem Elshaer Eman Mohamad El Nashar Eman Mohamad El Nashar Mansour Abdullah Alghamdi Mansour Abdullah Alghamdi Zainah Al-Qahtani Ahmed Nabawy Ahmed Nabawy Suppression of neuronal apoptosis and glial activation with modulation of Nrf2/HO-1 and NF-kB signaling by curcumin in streptozotocin-induced diabetic spinal cord central neuropathy Frontiers in Neuroanatomy diabetic neuropathy microglia astrocytes curcumin Nrf2 HO-1 |
| title | Suppression of neuronal apoptosis and glial activation with modulation of Nrf2/HO-1 and NF-kB signaling by curcumin in streptozotocin-induced diabetic spinal cord central neuropathy |
| title_full | Suppression of neuronal apoptosis and glial activation with modulation of Nrf2/HO-1 and NF-kB signaling by curcumin in streptozotocin-induced diabetic spinal cord central neuropathy |
| title_fullStr | Suppression of neuronal apoptosis and glial activation with modulation of Nrf2/HO-1 and NF-kB signaling by curcumin in streptozotocin-induced diabetic spinal cord central neuropathy |
| title_full_unstemmed | Suppression of neuronal apoptosis and glial activation with modulation of Nrf2/HO-1 and NF-kB signaling by curcumin in streptozotocin-induced diabetic spinal cord central neuropathy |
| title_short | Suppression of neuronal apoptosis and glial activation with modulation of Nrf2/HO-1 and NF-kB signaling by curcumin in streptozotocin-induced diabetic spinal cord central neuropathy |
| title_sort | suppression of neuronal apoptosis and glial activation with modulation of nrf2 ho 1 and nf kb signaling by curcumin in streptozotocin induced diabetic spinal cord central neuropathy |
| topic | diabetic neuropathy microglia astrocytes curcumin Nrf2 HO-1 |
| url | https://www.frontiersin.org/articles/10.3389/fnana.2023.1094301/full |
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