Apoptosis-Inducing Factor, Mitochondrion-Associated 3 (AIFM3) Protein Level in the Sera as a Prognostic Marker of Cholangiocarcinoma Patients
Prognosis of cholangiocarcinoma (CCA) patients is absolutely poor. Since improvement of prognosis and/or response to treatment by personalized and precision treatments requires earlier and precise diagnostic markers, discovery of prognostic markers attracts more attention. Apoptosis-inducing factor,...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/7/1021 |
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| author | Daraporn Chua-on Tanakorn Proungvitaya Doungdean Tummanatsakun Anchalee Techasen Temduang Limpaiboon Sittiruk Roytrakul Sopit Wongkham Chaisiri Wongkham Ongart Somintara Sakkarn Sangkhamanon Siriporn Proungvitaya |
| author_facet | Daraporn Chua-on Tanakorn Proungvitaya Doungdean Tummanatsakun Anchalee Techasen Temduang Limpaiboon Sittiruk Roytrakul Sopit Wongkham Chaisiri Wongkham Ongart Somintara Sakkarn Sangkhamanon Siriporn Proungvitaya |
| author_sort | Daraporn Chua-on |
| collection | DOAJ |
| description | Prognosis of cholangiocarcinoma (CCA) patients is absolutely poor. Since improvement of prognosis and/or response to treatment by personalized and precision treatments requires earlier and precise diagnostic markers, discovery of prognostic markers attracts more attention. Apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) is highly expressed in several cancers including CCA. The present study investigated whether the serum AIFM3 level can be used as a potential marker for CCA prognosis. For this purpose, we first determined secretory protein nature of AIFM3 using bioinformatic tools. The results show that although AIFM3 lacks signal peptide, it can be secreted into plasma/serum via an unconventional pathway. Then, the AIFM3 levels in the sera of 141 CCA patients and 70 healthy controls (HC) were measured using a semi-quantitative dot blot assay. The results show that the AIFM3 level in the sera of CCA group was significantly higher than that of HC. When correlation between serum AIFM3 levels and the clinicopathological parameters of CCA patients were examined, serum AIFM3 levels correlated significantly with lymph node metastasis, age, and the patients’ overall survival (OS). Higher AIFM3 levels were significantly associated with shorter OS, and only AIFM3 was an independent prognostic marker for CCA. In conclusion, AIFM3 can be used as a prognostic marker for CCA. |
| first_indexed | 2024-03-10T18:35:01Z |
| format | Article |
| id | doaj.art-d2fb0662bdfa4c9abdf99ccf6297ebea |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T18:35:01Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-d2fb0662bdfa4c9abdf99ccf6297ebea2023-11-20T06:22:24ZengMDPI AGBiomolecules2218-273X2020-07-01107102110.3390/biom10071021Apoptosis-Inducing Factor, Mitochondrion-Associated 3 (AIFM3) Protein Level in the Sera as a Prognostic Marker of Cholangiocarcinoma PatientsDaraporn Chua-on0Tanakorn Proungvitaya1Doungdean Tummanatsakun2Anchalee Techasen3Temduang Limpaiboon4Sittiruk Roytrakul5Sopit Wongkham6Chaisiri Wongkham7Ongart Somintara8Sakkarn Sangkhamanon9Siriporn Proungvitaya10Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandCentre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandCentre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandCentre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandCentre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, ThailandCholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandCholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandCentre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandPrognosis of cholangiocarcinoma (CCA) patients is absolutely poor. Since improvement of prognosis and/or response to treatment by personalized and precision treatments requires earlier and precise diagnostic markers, discovery of prognostic markers attracts more attention. Apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) is highly expressed in several cancers including CCA. The present study investigated whether the serum AIFM3 level can be used as a potential marker for CCA prognosis. For this purpose, we first determined secretory protein nature of AIFM3 using bioinformatic tools. The results show that although AIFM3 lacks signal peptide, it can be secreted into plasma/serum via an unconventional pathway. Then, the AIFM3 levels in the sera of 141 CCA patients and 70 healthy controls (HC) were measured using a semi-quantitative dot blot assay. The results show that the AIFM3 level in the sera of CCA group was significantly higher than that of HC. When correlation between serum AIFM3 levels and the clinicopathological parameters of CCA patients were examined, serum AIFM3 levels correlated significantly with lymph node metastasis, age, and the patients’ overall survival (OS). Higher AIFM3 levels were significantly associated with shorter OS, and only AIFM3 was an independent prognostic marker for CCA. In conclusion, AIFM3 can be used as a prognostic marker for CCA.https://www.mdpi.com/2218-273X/10/7/1021AIFM3serumdot blotprognostic markerCCA |
| spellingShingle | Daraporn Chua-on Tanakorn Proungvitaya Doungdean Tummanatsakun Anchalee Techasen Temduang Limpaiboon Sittiruk Roytrakul Sopit Wongkham Chaisiri Wongkham Ongart Somintara Sakkarn Sangkhamanon Siriporn Proungvitaya Apoptosis-Inducing Factor, Mitochondrion-Associated 3 (AIFM3) Protein Level in the Sera as a Prognostic Marker of Cholangiocarcinoma Patients Biomolecules AIFM3 serum dot blot prognostic marker CCA |
| title | Apoptosis-Inducing Factor, Mitochondrion-Associated 3 (AIFM3) Protein Level in the Sera as a Prognostic Marker of Cholangiocarcinoma Patients |
| title_full | Apoptosis-Inducing Factor, Mitochondrion-Associated 3 (AIFM3) Protein Level in the Sera as a Prognostic Marker of Cholangiocarcinoma Patients |
| title_fullStr | Apoptosis-Inducing Factor, Mitochondrion-Associated 3 (AIFM3) Protein Level in the Sera as a Prognostic Marker of Cholangiocarcinoma Patients |
| title_full_unstemmed | Apoptosis-Inducing Factor, Mitochondrion-Associated 3 (AIFM3) Protein Level in the Sera as a Prognostic Marker of Cholangiocarcinoma Patients |
| title_short | Apoptosis-Inducing Factor, Mitochondrion-Associated 3 (AIFM3) Protein Level in the Sera as a Prognostic Marker of Cholangiocarcinoma Patients |
| title_sort | apoptosis inducing factor mitochondrion associated 3 aifm3 protein level in the sera as a prognostic marker of cholangiocarcinoma patients |
| topic | AIFM3 serum dot blot prognostic marker CCA |
| url | https://www.mdpi.com/2218-273X/10/7/1021 |
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