<i>Saccharomyces cerevisiae</i> Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult Dogs
<i>Saccharomyces cerevisiae</i> yeast culture can be dehydrated, and it has a potential prebiotic effect. This study evaluated the effects of supplementing increasing levels of dehydrated yeast culture (DYC) of <i>Saccharomyces cerevisiae</i> (Original XPC™, Diamond V, Cedar...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/1/2 |
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| author | Karine de Melo Santos Larissa Wünsche Risolia Mariana Fragoso Rentas Andressa Rodrigues Amaral Roberta Bueno Ayres Rodrigues Maria Isabel Gonzalez Urrego Thiago Henrique Annibale Vendramini Ricardo Vieira Ventura Júlio César de Carvalho Balieiro Cristina de Oliveira Massoco João Paulo Fernandes Santos Cristiana Fonseca Ferreira Pontieri Marcio Antonio Brunetto |
| author_facet | Karine de Melo Santos Larissa Wünsche Risolia Mariana Fragoso Rentas Andressa Rodrigues Amaral Roberta Bueno Ayres Rodrigues Maria Isabel Gonzalez Urrego Thiago Henrique Annibale Vendramini Ricardo Vieira Ventura Júlio César de Carvalho Balieiro Cristina de Oliveira Massoco João Paulo Fernandes Santos Cristiana Fonseca Ferreira Pontieri Marcio Antonio Brunetto |
| author_sort | Karine de Melo Santos |
| collection | DOAJ |
| description | <i>Saccharomyces cerevisiae</i> yeast culture can be dehydrated, and it has a potential prebiotic effect. This study evaluated the effects of supplementing increasing levels of dehydrated yeast culture (DYC) of <i>Saccharomyces cerevisiae</i> (Original XPC™, Diamond V, Cedar Rapids, IA, USA) on fecal microbiota, nutrient digestibility, and fermentative and immunological parameters of healthy adult dogs. Eighteen adult male and female dogs with a mean body weight of 15.8 ± 7.37 kg were randomly assigned to three experimental treatments: CD (control diet), DYC 0.3 (control diet with 0.3% DYC) and DYC 0.6 (control diet with 0.6% DYC). After 21 days of acclimation, fecal samples were collected for analysis of nutrient digestibility, microbiota and fecal fermentation products. On the last day, the blood samples were collected for the analysis of immunological parameters. The microbiome profile was assessed by the Illumina sequencing method, which allowed identifying the population of each bacterial phylum and genus. The statistical analyses were performed using the SAS software and the Tukey test for multiple comparison (<i>p</i> < 0.05). Our results suggest that the addition of DYC increased the percentage of the phyla Actinobacteria and Firmicutes (<i>p</i> = 0.0048 and <i>p</i> < 0.0001, respectively) and reduced that of the phylum Fusobacteria (<i>p</i> = 0.0008). Regardless of the inclusion level, the yeast addition promoted reduction of the genera <i>Allobaculum</i> and <i>Fusobacterium</i> (<i>p</i> = 0.0265 and <i>p</i> = 0.0006, respectively) and increased (<i>p</i> = 0.0059) that of the genus <i>Clostridium</i>. At the highest prebiotic inclusion level (DYC 0.6), an increase (<i>p</i> = 0.0052) in the genus <i>Collinsella</i> and decrease (<i>p</i> = 0.0003) in <i>Prevotella</i> were observed. Besides that, the inclusion of the additive improved the apparent digestibility of the crude fiber and decreased the digestibility of crude protein, nitrogen-free extract and metabolizable energy (<i>p</i> < 0.05). There were no significant changes in the production of volatile organic compounds. However, an increase in propionate production was observed (<i>p</i> = 0.05). In addition, the inclusion of yeast resulted in an increased phagocytosis index in both treatments (<i>p</i> = 0.01). The addition of 0.3 and 0.6% DYC to the diet of dogs wase able to modulate the proportions of some phyla and genera in healthy dogs, in addition to yielding changes in nutrient digestibility, fermentative products and immunity in healthy adult dogs, indicating that this additive can modulate fecal microbiota and be included in dog nutrition. |
| first_indexed | 2024-03-10T01:31:02Z |
| format | Article |
| id | doaj.art-d2fea8cc05d74426a286ce6dac0aca99 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T01:31:02Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-d2fea8cc05d74426a286ce6dac0aca992023-11-23T13:42:46ZengMDPI AGFermentation2311-56372021-12-0181210.3390/fermentation8010002<i>Saccharomyces cerevisiae</i> Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult DogsKarine de Melo Santos0Larissa Wünsche Risolia1Mariana Fragoso Rentas2Andressa Rodrigues Amaral3Roberta Bueno Ayres Rodrigues4Maria Isabel Gonzalez Urrego5Thiago Henrique Annibale Vendramini6Ricardo Vieira Ventura7Júlio César de Carvalho Balieiro8Cristina de Oliveira Massoco9João Paulo Fernandes Santos10Cristiana Fonseca Ferreira Pontieri11Marcio Antonio Brunetto12Pet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, BrazilVeterinary Nutrology Service, Veterinary Teaching Hospital, School of Veterinary Medicine and Animal Science, University of São Paulo, Cidade Universitária, Sao Paulo 05508-270, SP, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, BrazilNutrition Development Center, Grandfood Industria e Comercio LTDA (Premier Pet), Dourado 13590-000, SP, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, Brazil<i>Saccharomyces cerevisiae</i> yeast culture can be dehydrated, and it has a potential prebiotic effect. This study evaluated the effects of supplementing increasing levels of dehydrated yeast culture (DYC) of <i>Saccharomyces cerevisiae</i> (Original XPC™, Diamond V, Cedar Rapids, IA, USA) on fecal microbiota, nutrient digestibility, and fermentative and immunological parameters of healthy adult dogs. Eighteen adult male and female dogs with a mean body weight of 15.8 ± 7.37 kg were randomly assigned to three experimental treatments: CD (control diet), DYC 0.3 (control diet with 0.3% DYC) and DYC 0.6 (control diet with 0.6% DYC). After 21 days of acclimation, fecal samples were collected for analysis of nutrient digestibility, microbiota and fecal fermentation products. On the last day, the blood samples were collected for the analysis of immunological parameters. The microbiome profile was assessed by the Illumina sequencing method, which allowed identifying the population of each bacterial phylum and genus. The statistical analyses were performed using the SAS software and the Tukey test for multiple comparison (<i>p</i> < 0.05). Our results suggest that the addition of DYC increased the percentage of the phyla Actinobacteria and Firmicutes (<i>p</i> = 0.0048 and <i>p</i> < 0.0001, respectively) and reduced that of the phylum Fusobacteria (<i>p</i> = 0.0008). Regardless of the inclusion level, the yeast addition promoted reduction of the genera <i>Allobaculum</i> and <i>Fusobacterium</i> (<i>p</i> = 0.0265 and <i>p</i> = 0.0006, respectively) and increased (<i>p</i> = 0.0059) that of the genus <i>Clostridium</i>. At the highest prebiotic inclusion level (DYC 0.6), an increase (<i>p</i> = 0.0052) in the genus <i>Collinsella</i> and decrease (<i>p</i> = 0.0003) in <i>Prevotella</i> were observed. Besides that, the inclusion of the additive improved the apparent digestibility of the crude fiber and decreased the digestibility of crude protein, nitrogen-free extract and metabolizable energy (<i>p</i> < 0.05). There were no significant changes in the production of volatile organic compounds. However, an increase in propionate production was observed (<i>p</i> = 0.05). In addition, the inclusion of yeast resulted in an increased phagocytosis index in both treatments (<i>p</i> = 0.01). The addition of 0.3 and 0.6% DYC to the diet of dogs wase able to modulate the proportions of some phyla and genera in healthy dogs, in addition to yielding changes in nutrient digestibility, fermentative products and immunity in healthy adult dogs, indicating that this additive can modulate fecal microbiota and be included in dog nutrition.https://www.mdpi.com/2311-5637/8/1/2bacteriabeta-glucanfermentationilluminamannan oligosaccharidesprebiotic |
| spellingShingle | Karine de Melo Santos Larissa Wünsche Risolia Mariana Fragoso Rentas Andressa Rodrigues Amaral Roberta Bueno Ayres Rodrigues Maria Isabel Gonzalez Urrego Thiago Henrique Annibale Vendramini Ricardo Vieira Ventura Júlio César de Carvalho Balieiro Cristina de Oliveira Massoco João Paulo Fernandes Santos Cristiana Fonseca Ferreira Pontieri Marcio Antonio Brunetto <i>Saccharomyces cerevisiae</i> Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult Dogs Fermentation bacteria beta-glucan fermentation illumina mannan oligosaccharides prebiotic |
| title | <i>Saccharomyces cerevisiae</i> Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult Dogs |
| title_full | <i>Saccharomyces cerevisiae</i> Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult Dogs |
| title_fullStr | <i>Saccharomyces cerevisiae</i> Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult Dogs |
| title_full_unstemmed | <i>Saccharomyces cerevisiae</i> Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult Dogs |
| title_short | <i>Saccharomyces cerevisiae</i> Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult Dogs |
| title_sort | i saccharomyces cerevisiae i dehydrated culture modulates fecal microbiota and improves innate immunity of adult dogs |
| topic | bacteria beta-glucan fermentation illumina mannan oligosaccharides prebiotic |
| url | https://www.mdpi.com/2311-5637/8/1/2 |
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