SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus
Sugarcane smut caused by Sporisorium scitamineum is a destructive disease responsible for significant losses in sugarcane production worldwide. However, the mechanisms underlying the pathogenicity of this fungus remain largely unknown. In this study, we found that the disruption of the SsRSS1 gene,...
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| Language: | English |
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Elsevier
2023-07-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922002568 |
| _version_ | 1797774844003090432 |
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| author | Hao-yang ZHANG Yan-fang YANG Feng GUO Xiao-rui SHEN Shan LU Bao-shan CHEN |
| author_facet | Hao-yang ZHANG Yan-fang YANG Feng GUO Xiao-rui SHEN Shan LU Bao-shan CHEN |
| author_sort | Hao-yang ZHANG |
| collection | DOAJ |
| description | Sugarcane smut caused by Sporisorium scitamineum is a destructive disease responsible for significant losses in sugarcane production worldwide. However, the mechanisms underlying the pathogenicity of this fungus remain largely unknown. In this study, we found that the disruption of the SsRSS1 gene, which encodes a salicylic acid (SA) sensing regulator, does not affect phenotypic traits such as the morphology, growth rate, and sexual mating ability of haploid basidiospores, but rather reduces the tolerance of basidiospores to SA stress by blocking the induction of SsSRG1, a gene encoding a SA response protein in S. scitamineum. SsRSS1 deletion resulted in the attenuation of the virulence of the fungus. In addition to a significant reduction in whip formation, a portion of plantlets (18.3%) inoculated with the ΔSsRSS1 strains were found to be infected but failed to produce whips for up to 90 days post-inoculation. However, the development of hyphae and teliospore from the ΔSsRSS1-infected plants that formed whips seemed indistinguishable from that in the wild-type-infected plants. Combined, our findings suggested that SsRss1 is required for maintaining fungal fitness in planta by counteracting SA stress. |
| first_indexed | 2024-03-12T22:27:07Z |
| format | Article |
| id | doaj.art-d2fff60b07f34516b750adb44d3562a9 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-12T22:27:07Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-d2fff60b07f34516b750adb44d3562a92023-07-22T04:51:46ZengElsevierJournal of Integrative Agriculture2095-31192023-07-0122721262137SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungusHao-yang ZHANG0Yan-fang YANG1Feng GUO2Xiao-rui SHEN3Shan LU4Bao-shan CHEN5College of Agriculture/Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning 530004, P.R.ChinaCollege of Agriculture/Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning 530004, P.R.ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/College of Life Science and Technology, Guangxi University, Nanning 530004, P.R.ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/College of Life Science and Technology, Guangxi University, Nanning 530004, P.R.ChinaCollege of Agriculture/Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning 530004, P.R.China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/College of Life Science and Technology, Guangxi University, Nanning 530004, P.R.China; Ministry and Province Co-sponsored Collaborative Innovation Center for Sugarcane and Sugar Industry, Guangxi University, Nanning 530004, P.R.China; Correspondence LU Shan, Tel: +86-771-3394554College of Agriculture/Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning 530004, P.R.China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/College of Life Science and Technology, Guangxi University, Nanning 530004, P.R.China; Ministry and Province Co-sponsored Collaborative Innovation Center for Sugarcane and Sugar Industry, Guangxi University, Nanning 530004, P.R.China; Correspondence CHEN Bao-shan, Tel: +86-771-3225260Sugarcane smut caused by Sporisorium scitamineum is a destructive disease responsible for significant losses in sugarcane production worldwide. However, the mechanisms underlying the pathogenicity of this fungus remain largely unknown. In this study, we found that the disruption of the SsRSS1 gene, which encodes a salicylic acid (SA) sensing regulator, does not affect phenotypic traits such as the morphology, growth rate, and sexual mating ability of haploid basidiospores, but rather reduces the tolerance of basidiospores to SA stress by blocking the induction of SsSRG1, a gene encoding a SA response protein in S. scitamineum. SsRSS1 deletion resulted in the attenuation of the virulence of the fungus. In addition to a significant reduction in whip formation, a portion of plantlets (18.3%) inoculated with the ΔSsRSS1 strains were found to be infected but failed to produce whips for up to 90 days post-inoculation. However, the development of hyphae and teliospore from the ΔSsRSS1-infected plants that formed whips seemed indistinguishable from that in the wild-type-infected plants. Combined, our findings suggested that SsRss1 is required for maintaining fungal fitness in planta by counteracting SA stress.http://www.sciencedirect.com/science/article/pii/S2095311922002568Sporisorium scitamineumSsRSS1pathogenicitysalicylic acid response |
| spellingShingle | Hao-yang ZHANG Yan-fang YANG Feng GUO Xiao-rui SHEN Shan LU Bao-shan CHEN SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus Journal of Integrative Agriculture Sporisorium scitamineum SsRSS1 pathogenicity salicylic acid response |
| title | SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus |
| title_full | SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus |
| title_fullStr | SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus |
| title_full_unstemmed | SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus |
| title_short | SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus |
| title_sort | ssrss1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus |
| topic | Sporisorium scitamineum SsRSS1 pathogenicity salicylic acid response |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922002568 |
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