Performance and Microbial Community analysis on denitrification of electronic wastewater using various External Carbon Source
Objectives In the biological denitrification process of electronic wastewater, the denitrification performance of various types of external carbon sources and the changes in the bacterial community before and after denitrification were evaluated through NGS analysis. Methods After selecting 6 types...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Society of Environmental Engineers
2024-02-01
|
| Series: | 대한환경공학회지 |
| Subjects: | |
| Online Access: | http://www.jksee.or.kr/upload/pdf/KSEE-2024-46-2-48.pdf |
| _version_ | 1797277672295890944 |
|---|---|
| author | Hyunjun Song Taegeun Kim HyeonJung Cha SungYeop Jung |
| author_facet | Hyunjun Song Taegeun Kim HyeonJung Cha SungYeop Jung |
| author_sort | Hyunjun Song |
| collection | DOAJ |
| description | Objectives In the biological denitrification process of electronic wastewater, the denitrification performance of various types of external carbon sources and the changes in the bacterial community before and after denitrification were evaluated through NGS analysis. Methods After selecting 6 types of external carbon sources, the concentration of nitrate nitrogen was analyzed for 6 hours under anoxic conditions with a C/N ratio of 4 to evaluate the denitrification rate, and the changes in the community distribution of bacteria before and after denitrification were confirmed through NGS analysis. Results and Discussion As a result of comparing the denitrification performance of various external carbon sources, Ethylene glycol(EG) was the best at 79.9% after 6 hours, and the specific denitrification rate(SDNR) was 1.000mg NO3--N removal/g MLVSS·hr. The bacterial community change using NGS was distributed more than 90% of 10 phylums at the phylum level, and Proteobacteria, Saccharibacteria, and Chloroflexi were dominant, and among them, Saccharibacteria and Chloroflexi were confirmed to be bacteria contributing to denitrification. At class and genus level, when a external carbon source was added, the number of γ-proteobacteria increased in all experimental conditions, but the distribution of denitrifying bacteria was less than 1.27%, indicating that various bacteria contributed to denitrification. Conclusion In the case of Ethylene glycol(EG), it is judged that it can be used as an external carbon source, and there was no significant change in the community depending on the type of carbon source injected, and various bacteria such as Saccharibacteria and Chloroflexi contributed to denitrification and eliminated nitrogen pollutants. |
| first_indexed | 2024-03-07T15:51:46Z |
| format | Article |
| id | doaj.art-d30411a6d9b34118b47fe8ebba882510 |
| institution | Directory Open Access Journal |
| issn | 1225-5025 2383-7810 |
| language | English |
| last_indexed | 2024-03-07T15:51:46Z |
| publishDate | 2024-02-01 |
| publisher | Korean Society of Environmental Engineers |
| record_format | Article |
| series | 대한환경공학회지 |
| spelling | doaj.art-d30411a6d9b34118b47fe8ebba8825102024-03-05T00:07:03ZengKorean Society of Environmental Engineers대한환경공학회지1225-50252383-78102024-02-01462485610.4491/KSEE.2024.46.2.484499Performance and Microbial Community analysis on denitrification of electronic wastewater using various External Carbon SourceHyunjun Song0Taegeun Kim1HyeonJung Cha2SungYeop Jung3JCnM Co., Ltd. Institute of technologyJCnM Co., Ltd. Institute of technologyJCnM Co., Ltd. Institute of technologyJCnM Co., Ltd. Institute of technologyObjectives In the biological denitrification process of electronic wastewater, the denitrification performance of various types of external carbon sources and the changes in the bacterial community before and after denitrification were evaluated through NGS analysis. Methods After selecting 6 types of external carbon sources, the concentration of nitrate nitrogen was analyzed for 6 hours under anoxic conditions with a C/N ratio of 4 to evaluate the denitrification rate, and the changes in the community distribution of bacteria before and after denitrification were confirmed through NGS analysis. Results and Discussion As a result of comparing the denitrification performance of various external carbon sources, Ethylene glycol(EG) was the best at 79.9% after 6 hours, and the specific denitrification rate(SDNR) was 1.000mg NO3--N removal/g MLVSS·hr. The bacterial community change using NGS was distributed more than 90% of 10 phylums at the phylum level, and Proteobacteria, Saccharibacteria, and Chloroflexi were dominant, and among them, Saccharibacteria and Chloroflexi were confirmed to be bacteria contributing to denitrification. At class and genus level, when a external carbon source was added, the number of γ-proteobacteria increased in all experimental conditions, but the distribution of denitrifying bacteria was less than 1.27%, indicating that various bacteria contributed to denitrification. Conclusion In the case of Ethylene glycol(EG), it is judged that it can be used as an external carbon source, and there was no significant change in the community depending on the type of carbon source injected, and various bacteria such as Saccharibacteria and Chloroflexi contributed to denitrification and eliminated nitrogen pollutants.http://www.jksee.or.kr/upload/pdf/KSEE-2024-46-2-48.pdfelectronic wastewaterexternal carbon sourcesdenitrificationnext generation sequencingbacterial communities |
| spellingShingle | Hyunjun Song Taegeun Kim HyeonJung Cha SungYeop Jung Performance and Microbial Community analysis on denitrification of electronic wastewater using various External Carbon Source 대한환경공학회지 electronic wastewater external carbon sources denitrification next generation sequencing bacterial communities |
| title | Performance and Microbial Community analysis on denitrification of electronic wastewater using various External Carbon Source |
| title_full | Performance and Microbial Community analysis on denitrification of electronic wastewater using various External Carbon Source |
| title_fullStr | Performance and Microbial Community analysis on denitrification of electronic wastewater using various External Carbon Source |
| title_full_unstemmed | Performance and Microbial Community analysis on denitrification of electronic wastewater using various External Carbon Source |
| title_short | Performance and Microbial Community analysis on denitrification of electronic wastewater using various External Carbon Source |
| title_sort | performance and microbial community analysis on denitrification of electronic wastewater using various external carbon source |
| topic | electronic wastewater external carbon sources denitrification next generation sequencing bacterial communities |
| url | http://www.jksee.or.kr/upload/pdf/KSEE-2024-46-2-48.pdf |
| work_keys_str_mv | AT hyunjunsong performanceandmicrobialcommunityanalysisondenitrificationofelectronicwastewaterusingvariousexternalcarbonsource AT taegeunkim performanceandmicrobialcommunityanalysisondenitrificationofelectronicwastewaterusingvariousexternalcarbonsource AT hyeonjungcha performanceandmicrobialcommunityanalysisondenitrificationofelectronicwastewaterusingvariousexternalcarbonsource AT sungyeopjung performanceandmicrobialcommunityanalysisondenitrificationofelectronicwastewaterusingvariousexternalcarbonsource |