Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs
Objective: To explore the possible mechanism of improving the imiquimod (IMQ)-induced psoriasis-like inflammation by using polyethylene glycol (PEG) ointment.Methods: We evaluated the appearance of psoriasis lesions by Psoriasis Area and Severity Index (PASI), observed the epidermal proliferation by...
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2020.560579/full |
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| author | Yan Lu Yan Lu Yi Xiao Yi Xiao Ming-Zhu Yin Ming-Zhu Yin Xing-Chen Zhou Li-Sha Wu Li-Sha Wu Wang-Qing Chen Yan Luo Yan Luo Ye-Hong Kuang Ye-Hong Kuang Wu Zhu Wu Zhu |
| author_facet | Yan Lu Yan Lu Yi Xiao Yi Xiao Ming-Zhu Yin Ming-Zhu Yin Xing-Chen Zhou Li-Sha Wu Li-Sha Wu Wang-Qing Chen Yan Luo Yan Luo Ye-Hong Kuang Ye-Hong Kuang Wu Zhu Wu Zhu |
| author_sort | Yan Lu |
| collection | DOAJ |
| description | Objective: To explore the possible mechanism of improving the imiquimod (IMQ)-induced psoriasis-like inflammation by using polyethylene glycol (PEG) ointment.Methods: We evaluated the appearance of psoriasis lesions by Psoriasis Area and Severity Index (PASI), observed the epidermal proliferation by histopathological staining and immunohistochemical staining, and explored the key molecules and signaling pathways of improving psoriasis-like inflammation treated with PEG ointment by RNA sequencing. Finally, we verified the expression of inflammatory cells and inflammatory factors by flow cytometry, immunohistochemical staining, and Q-PCR.Results: PEG ointment could improve the appearance of psoriasis lesions and the epidermis thickness of psoriasis mouse, inhibit the proliferation of keratinocytes, and down-regulate the relative mRNA levels of IL-23, IL-22, IL-6, IL-17C, IL-17F, S100A7, S100A8, S100A9, CXCL1, CXCL2, and IL-1β in the skin lesions of psoriasis mouse by down-regulating the numbers of myeloid-derived suppressor cells (MDSCs) and T helper 17 (Th17) cells.Conclusion: PEG ointment could improve the IMQ-induced psoriasis-like inflammation by down-regulating the functions of Th17 cells and MDSCs. |
| first_indexed | 2024-12-16T18:43:10Z |
| format | Article |
| id | doaj.art-d306c13502eb4711a24dd6ba6e745796 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-12-16T18:43:10Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-d306c13502eb4711a24dd6ba6e7457962022-12-21T22:20:57ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2021-03-01710.3389/fmed.2020.560579560579Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCsYan Lu0Yan Lu1Yi Xiao2Yi Xiao3Ming-Zhu Yin4Ming-Zhu Yin5Xing-Chen Zhou6Li-Sha Wu7Li-Sha Wu8Wang-Qing Chen9Yan Luo10Yan Luo11Ye-Hong Kuang12Ye-Hong Kuang13Wu Zhu14Wu Zhu15Department of Dermatology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Dermatology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Dermatology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, ChinaPharmacy, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, ChinaInstitute of Medical Science, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Dermatology, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Dermatology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Dermatology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Dermatology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, ChinaObjective: To explore the possible mechanism of improving the imiquimod (IMQ)-induced psoriasis-like inflammation by using polyethylene glycol (PEG) ointment.Methods: We evaluated the appearance of psoriasis lesions by Psoriasis Area and Severity Index (PASI), observed the epidermal proliferation by histopathological staining and immunohistochemical staining, and explored the key molecules and signaling pathways of improving psoriasis-like inflammation treated with PEG ointment by RNA sequencing. Finally, we verified the expression of inflammatory cells and inflammatory factors by flow cytometry, immunohistochemical staining, and Q-PCR.Results: PEG ointment could improve the appearance of psoriasis lesions and the epidermis thickness of psoriasis mouse, inhibit the proliferation of keratinocytes, and down-regulate the relative mRNA levels of IL-23, IL-22, IL-6, IL-17C, IL-17F, S100A7, S100A8, S100A9, CXCL1, CXCL2, and IL-1β in the skin lesions of psoriasis mouse by down-regulating the numbers of myeloid-derived suppressor cells (MDSCs) and T helper 17 (Th17) cells.Conclusion: PEG ointment could improve the IMQ-induced psoriasis-like inflammation by down-regulating the functions of Th17 cells and MDSCs.https://www.frontiersin.org/articles/10.3389/fmed.2020.560579/fullpsoriasisIMQPEGMDSCsTh17 |
| spellingShingle | Yan Lu Yan Lu Yi Xiao Yi Xiao Ming-Zhu Yin Ming-Zhu Yin Xing-Chen Zhou Li-Sha Wu Li-Sha Wu Wang-Qing Chen Yan Luo Yan Luo Ye-Hong Kuang Ye-Hong Kuang Wu Zhu Wu Zhu Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs Frontiers in Medicine psoriasis IMQ PEG MDSCs Th17 |
| title | Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs |
| title_full | Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs |
| title_fullStr | Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs |
| title_full_unstemmed | Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs |
| title_short | Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs |
| title_sort | polyethylene glycol ointment alleviates psoriasis like inflammation through down regulating the function of th17 cells and mdscs |
| topic | psoriasis IMQ PEG MDSCs Th17 |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2020.560579/full |
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