Apple Fruit Size QTLs on Chromosomes 8 and 16 Characterized in ‘Honeycrisp’-Derived Germplasm
Multiple quantitative trait loci (QTLs) associated with fruit size have been reported in apple <i>(Malus domestica</i> Borkh.); however, few have been fully characterized and/or validated. A pedigree-based QTL analysis approach was used to examine the genetic basis of fruit weight in ‘Ho...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/6/1279 |
| _version_ | 1797490972107472896 |
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| author | Sarah A. Kostick James J. Luby |
| author_facet | Sarah A. Kostick James J. Luby |
| author_sort | Sarah A. Kostick |
| collection | DOAJ |
| description | Multiple quantitative trait loci (QTLs) associated with fruit size have been reported in apple <i>(Malus domestica</i> Borkh.); however, few have been fully characterized and/or validated. A pedigree-based QTL analysis approach was used to examine the genetic basis of fruit weight in ‘Honeycrisp’-derived germplasm. Fourteen breeding parents were represented by 814 offspring from 13 full-sib families with breeding parents ‘Honeycrisp’ and ‘Minneiska’ being highly represented. Historical fruit weight data and curated genome-wide single nucleotide polymorphism (SNP) data were leveraged to map QTLs to chromosomes (Chrs) 8 and 16, which together accounted for 15% of the phenotypic variation. The Chr 16 QTL colocalized with other important Chr 16 trait loci. ‘Honeycrisp’ inherited two low fruit weight haplotypes at the Chr 8 QTL from progenitors ‘Northern Spy’ and ‘Grimes Golden’. At the Chr 16 QTL, ‘Honeycrisp’ inherited a low fruit weight haplotype from ‘Frostbite’ and a high fruit weight haplotype from ‘Duchess of Oldenburg’. The small-fruited ‘Honeycrisp’ progenitor ‘Frostbite’ had three low fruit weight haplotypes across the two QTLs. Non-additive interactions were observed at and across QTLs. Results will enable more informed parent selection and/or development of trait-predictive DNA tests for use in apple breeding programs. |
| first_indexed | 2024-03-10T00:40:43Z |
| format | Article |
| id | doaj.art-d309e7be6d194442a46d23feaaa7e33b |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T00:40:43Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-d309e7be6d194442a46d23feaaa7e33b2023-11-23T15:09:09ZengMDPI AGAgronomy2073-43952022-05-01126127910.3390/agronomy12061279Apple Fruit Size QTLs on Chromosomes 8 and 16 Characterized in ‘Honeycrisp’-Derived GermplasmSarah A. Kostick0James J. Luby1Department of Horticultural Science, University of Minnesota, Saint Paul, MN 55108, USADepartment of Horticultural Science, University of Minnesota, Saint Paul, MN 55108, USAMultiple quantitative trait loci (QTLs) associated with fruit size have been reported in apple <i>(Malus domestica</i> Borkh.); however, few have been fully characterized and/or validated. A pedigree-based QTL analysis approach was used to examine the genetic basis of fruit weight in ‘Honeycrisp’-derived germplasm. Fourteen breeding parents were represented by 814 offspring from 13 full-sib families with breeding parents ‘Honeycrisp’ and ‘Minneiska’ being highly represented. Historical fruit weight data and curated genome-wide single nucleotide polymorphism (SNP) data were leveraged to map QTLs to chromosomes (Chrs) 8 and 16, which together accounted for 15% of the phenotypic variation. The Chr 16 QTL colocalized with other important Chr 16 trait loci. ‘Honeycrisp’ inherited two low fruit weight haplotypes at the Chr 8 QTL from progenitors ‘Northern Spy’ and ‘Grimes Golden’. At the Chr 16 QTL, ‘Honeycrisp’ inherited a low fruit weight haplotype from ‘Frostbite’ and a high fruit weight haplotype from ‘Duchess of Oldenburg’. The small-fruited ‘Honeycrisp’ progenitor ‘Frostbite’ had three low fruit weight haplotypes across the two QTLs. Non-additive interactions were observed at and across QTLs. Results will enable more informed parent selection and/or development of trait-predictive DNA tests for use in apple breeding programs.https://www.mdpi.com/2073-4395/12/6/1279<i>Malus domestica</i> Borkh.quantitative trait locuspedigree-based analysisDNA-informed breeding |
| spellingShingle | Sarah A. Kostick James J. Luby Apple Fruit Size QTLs on Chromosomes 8 and 16 Characterized in ‘Honeycrisp’-Derived Germplasm Agronomy <i>Malus domestica</i> Borkh. quantitative trait locus pedigree-based analysis DNA-informed breeding |
| title | Apple Fruit Size QTLs on Chromosomes 8 and 16 Characterized in ‘Honeycrisp’-Derived Germplasm |
| title_full | Apple Fruit Size QTLs on Chromosomes 8 and 16 Characterized in ‘Honeycrisp’-Derived Germplasm |
| title_fullStr | Apple Fruit Size QTLs on Chromosomes 8 and 16 Characterized in ‘Honeycrisp’-Derived Germplasm |
| title_full_unstemmed | Apple Fruit Size QTLs on Chromosomes 8 and 16 Characterized in ‘Honeycrisp’-Derived Germplasm |
| title_short | Apple Fruit Size QTLs on Chromosomes 8 and 16 Characterized in ‘Honeycrisp’-Derived Germplasm |
| title_sort | apple fruit size qtls on chromosomes 8 and 16 characterized in honeycrisp derived germplasm |
| topic | <i>Malus domestica</i> Borkh. quantitative trait locus pedigree-based analysis DNA-informed breeding |
| url | https://www.mdpi.com/2073-4395/12/6/1279 |
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