Characterization of two novel colistin resistance gene mcr-1 variants originated from Moraxella spp.
This study aimed to characterize two novel mcr-1 variants, mcr-1.35 and mcr-1.36, which originated from Moraxella spp. that were isolated from diseased pigs in China. The Moraxella spp. carrying novel mcr-1 variants were subjected to whole-genome sequencing (WGS) and phylogenetic analysis based on t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1153740/full |
| _version_ | 1797826376389099520 |
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| author | Yongliang Che Yongliang Che Renjie Wu Renjie Wu Hongjie Li Longbai Wang Longbai Wang Xuemin Wu Xuemin Wu Qiuyong Chen Qiuyong Chen Rujing Chen Rujing Chen Lunjiang Zhou Lunjiang Zhou |
| author_facet | Yongliang Che Yongliang Che Renjie Wu Renjie Wu Hongjie Li Longbai Wang Longbai Wang Xuemin Wu Xuemin Wu Qiuyong Chen Qiuyong Chen Rujing Chen Rujing Chen Lunjiang Zhou Lunjiang Zhou |
| author_sort | Yongliang Che |
| collection | DOAJ |
| description | This study aimed to characterize two novel mcr-1 variants, mcr-1.35 and mcr-1.36, which originated from Moraxella spp. that were isolated from diseased pigs in China. The Moraxella spp. carrying novel mcr-1 variants were subjected to whole-genome sequencing (WGS) and phylogenetic analysis based on the 16S rRNA gene. The mcr-1 variants mcr-1.35 and mcr-1.36 were characterized using phylogenetic analysis, a comparison of genetic environments, and protein structure prediction. The WGS indicated that two novel mcr-1 variants were located in the chromosomes of three Moraxella spp. with a genetic environment of mcr-1-pap2. In addition to the novel colistin resistance genes mcr-1.35 and mcr-1.36, the three Moraxella spp. contained other antimicrobial resistance genes, including aac(3)-IId, tet(O), sul2, floR, and blaROB-3. A functional cloning assay indicated that either the mcr-1.35 or mcr-1.36 gene could confer resistance to colistin in Escherichia coli DH5α and JM109. The nucleotide sequences of mcr-1.35 and mcr-1.36 presented 95.33 and 95.33% identities, respectively, to mcr-1.1. The phylogenetic analysis showed that mcr-1.35 and mcr-1.36 were derived from Moraxella spp. that belonged to subclades that were different from those of the mcr-1 variants (mcr-1.1 to mcr-1.34 except mcr-1.10) originating from Enterobacteriaceae. The deduced amino acid sequences of MCR-1.35 (MCR-1.36) showed 96.67% (96.49%), 82.59% (82.04%), 84.07% (83.52%), 55.52% (55.17%), 59.75% (59.57%), and 61.88% (61.69%) identity to MCR-1.10, MCR-2.2, MCR-6.1, MCR-LIN, MCR-OSL, and MCR-POR, respectively, that originated from Moraxella sp. Notably, protein structure alignment showed only a few changes in amino acid residues between MCR-1.1 and MCR-1.35, as well as between MCR-1.1 and MCR-1.36. In conclusion, this study identified Moraxella spp. carrying two novel mcr-1 variants, mcr-1.35 and mcr-1.36, conferring resistance to colistin, which were isolated from pig farms in China. In addition, mcr-like variants were observed to be located in the chromosomes of some species of Moraxella isolated from pig samples. |
| first_indexed | 2024-04-09T12:30:53Z |
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| id | doaj.art-d30acde2c0124da58b167f904adc178e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T12:30:53Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d30acde2c0124da58b167f904adc178e2023-05-16T05:22:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11537401153740Characterization of two novel colistin resistance gene mcr-1 variants originated from Moraxella spp.Yongliang Che0Yongliang Che1Renjie Wu2Renjie Wu3Hongjie Li4Longbai Wang5Longbai Wang6Xuemin Wu7Xuemin Wu8Qiuyong Chen9Qiuyong Chen10Rujing Chen11Rujing Chen12Lunjiang Zhou13Lunjiang Zhou14Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaThis study aimed to characterize two novel mcr-1 variants, mcr-1.35 and mcr-1.36, which originated from Moraxella spp. that were isolated from diseased pigs in China. The Moraxella spp. carrying novel mcr-1 variants were subjected to whole-genome sequencing (WGS) and phylogenetic analysis based on the 16S rRNA gene. The mcr-1 variants mcr-1.35 and mcr-1.36 were characterized using phylogenetic analysis, a comparison of genetic environments, and protein structure prediction. The WGS indicated that two novel mcr-1 variants were located in the chromosomes of three Moraxella spp. with a genetic environment of mcr-1-pap2. In addition to the novel colistin resistance genes mcr-1.35 and mcr-1.36, the three Moraxella spp. contained other antimicrobial resistance genes, including aac(3)-IId, tet(O), sul2, floR, and blaROB-3. A functional cloning assay indicated that either the mcr-1.35 or mcr-1.36 gene could confer resistance to colistin in Escherichia coli DH5α and JM109. The nucleotide sequences of mcr-1.35 and mcr-1.36 presented 95.33 and 95.33% identities, respectively, to mcr-1.1. The phylogenetic analysis showed that mcr-1.35 and mcr-1.36 were derived from Moraxella spp. that belonged to subclades that were different from those of the mcr-1 variants (mcr-1.1 to mcr-1.34 except mcr-1.10) originating from Enterobacteriaceae. The deduced amino acid sequences of MCR-1.35 (MCR-1.36) showed 96.67% (96.49%), 82.59% (82.04%), 84.07% (83.52%), 55.52% (55.17%), 59.75% (59.57%), and 61.88% (61.69%) identity to MCR-1.10, MCR-2.2, MCR-6.1, MCR-LIN, MCR-OSL, and MCR-POR, respectively, that originated from Moraxella sp. Notably, protein structure alignment showed only a few changes in amino acid residues between MCR-1.1 and MCR-1.35, as well as between MCR-1.1 and MCR-1.36. In conclusion, this study identified Moraxella spp. carrying two novel mcr-1 variants, mcr-1.35 and mcr-1.36, conferring resistance to colistin, which were isolated from pig farms in China. In addition, mcr-like variants were observed to be located in the chromosomes of some species of Moraxella isolated from pig samples.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1153740/fullpigMoraxellamcr-1 variantcolistinresistancemcr-1.35 |
| spellingShingle | Yongliang Che Yongliang Che Renjie Wu Renjie Wu Hongjie Li Longbai Wang Longbai Wang Xuemin Wu Xuemin Wu Qiuyong Chen Qiuyong Chen Rujing Chen Rujing Chen Lunjiang Zhou Lunjiang Zhou Characterization of two novel colistin resistance gene mcr-1 variants originated from Moraxella spp. Frontiers in Microbiology pig Moraxella mcr-1 variant colistin resistance mcr-1.35 |
| title | Characterization of two novel colistin resistance gene mcr-1 variants originated from Moraxella spp. |
| title_full | Characterization of two novel colistin resistance gene mcr-1 variants originated from Moraxella spp. |
| title_fullStr | Characterization of two novel colistin resistance gene mcr-1 variants originated from Moraxella spp. |
| title_full_unstemmed | Characterization of two novel colistin resistance gene mcr-1 variants originated from Moraxella spp. |
| title_short | Characterization of two novel colistin resistance gene mcr-1 variants originated from Moraxella spp. |
| title_sort | characterization of two novel colistin resistance gene mcr 1 variants originated from moraxella spp |
| topic | pig Moraxella mcr-1 variant colistin resistance mcr-1.35 |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1153740/full |
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