Comparative biological activity of abamectin formulations on root-knot nematodes (Meloidogyne spp.) infecting cucumber plants: in vivo and in vitro
Abstract The root-knot nematodes (Meloidogyne spp.) are considered one of the most destructive diseases in the world. In Egypt, farmers primarily rely on chemical nematicides, which have become costly to control. Currently, abamectin is a bio-based pesticide used as an alternative tool against Meloi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39324-x |
| _version_ | 1797752799121899520 |
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| author | Magdy A. Massoud Abdel Fattah S. A. Saad Mohamed S. Khalil Mosher Zakaria Shady Selim |
| author_facet | Magdy A. Massoud Abdel Fattah S. A. Saad Mohamed S. Khalil Mosher Zakaria Shady Selim |
| author_sort | Magdy A. Massoud |
| collection | DOAJ |
| description | Abstract The root-knot nematodes (Meloidogyne spp.) are considered one of the most destructive diseases in the world. In Egypt, farmers primarily rely on chemical nematicides, which have become costly to control. Currently, abamectin is a bio-based pesticide used as an alternative tool against Meloidogyne spp. on cucumber plants (Cucumis sativus L.). During the current research, four tested abamectin formulations were DIVA (1.8% EW), RIOMECTIN (5% ME), AGRIMEC GOLD (8.4% SC) and ZORO (3.6% EC) compared with two reference nematicides namely, CROP NEMA (5% CS) and TERVIGO (2% SC). The main results showed that, in vitro study elucidated that the most effective formulations of abamectin as a larvicidal were EW with LC50 value of 21.66 µg ml−1. However, in the egg hatching test, the formulations of abamectin SC (2%) and EW were the most effective in reducing egg hatching, with LC50 values of 12.83 and 13.57 µg ml−1. The calculated relative potency values showed diversity depending on the two referenced nematicides. On the other hand, in vivo study, the results indicated that, all tested formulations of abamectin recorded general mean reductions in root galls (23.05–75.23%), egg masses (14.46–65.63%). Moreover, the total population density declined by 39.24–87.08%. Furthermore, the influence of abamectin formulations, in the presence of root-knot nematodes, on the growth of cucumber plants parameters, such as root dry weight, root length, root radius, root surface area, shoot dry weight and shoot height, as well as the content of macro-elements (N, P and K) exhibited varying levels of response. |
| first_indexed | 2024-03-12T17:08:39Z |
| format | Article |
| id | doaj.art-d30b25eb7fed4d85aeb1576a10459829 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T17:08:39Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d30b25eb7fed4d85aeb1576a104598292023-08-06T11:15:04ZengNature PortfolioScientific Reports2045-23222023-07-0113111410.1038/s41598-023-39324-xComparative biological activity of abamectin formulations on root-knot nematodes (Meloidogyne spp.) infecting cucumber plants: in vivo and in vitroMagdy A. Massoud0Abdel Fattah S. A. Saad1Mohamed S. Khalil2Mosher Zakaria3Shady Selim4Plant Protection Department, Faculty of Agriculture (Saba-Basha), Alexandria UniversityPlant Protection Department, Faculty of Agriculture (Saba-Basha), Alexandria UniversityFungicides, Bactericides and Nematicides Department, Central Agricultural Pesticides Laboratory, Agricultural Research CenterPlant Protection Department, Faculty of Agriculture (Saba-Basha), Alexandria UniversityFaculty of Desert and Environmental Agriculture, Department of Pesticide Chemistry and Technology, Matrouh UniversityAbstract The root-knot nematodes (Meloidogyne spp.) are considered one of the most destructive diseases in the world. In Egypt, farmers primarily rely on chemical nematicides, which have become costly to control. Currently, abamectin is a bio-based pesticide used as an alternative tool against Meloidogyne spp. on cucumber plants (Cucumis sativus L.). During the current research, four tested abamectin formulations were DIVA (1.8% EW), RIOMECTIN (5% ME), AGRIMEC GOLD (8.4% SC) and ZORO (3.6% EC) compared with two reference nematicides namely, CROP NEMA (5% CS) and TERVIGO (2% SC). The main results showed that, in vitro study elucidated that the most effective formulations of abamectin as a larvicidal were EW with LC50 value of 21.66 µg ml−1. However, in the egg hatching test, the formulations of abamectin SC (2%) and EW were the most effective in reducing egg hatching, with LC50 values of 12.83 and 13.57 µg ml−1. The calculated relative potency values showed diversity depending on the two referenced nematicides. On the other hand, in vivo study, the results indicated that, all tested formulations of abamectin recorded general mean reductions in root galls (23.05–75.23%), egg masses (14.46–65.63%). Moreover, the total population density declined by 39.24–87.08%. Furthermore, the influence of abamectin formulations, in the presence of root-knot nematodes, on the growth of cucumber plants parameters, such as root dry weight, root length, root radius, root surface area, shoot dry weight and shoot height, as well as the content of macro-elements (N, P and K) exhibited varying levels of response.https://doi.org/10.1038/s41598-023-39324-x |
| spellingShingle | Magdy A. Massoud Abdel Fattah S. A. Saad Mohamed S. Khalil Mosher Zakaria Shady Selim Comparative biological activity of abamectin formulations on root-knot nematodes (Meloidogyne spp.) infecting cucumber plants: in vivo and in vitro Scientific Reports |
| title | Comparative biological activity of abamectin formulations on root-knot nematodes (Meloidogyne spp.) infecting cucumber plants: in vivo and in vitro |
| title_full | Comparative biological activity of abamectin formulations on root-knot nematodes (Meloidogyne spp.) infecting cucumber plants: in vivo and in vitro |
| title_fullStr | Comparative biological activity of abamectin formulations on root-knot nematodes (Meloidogyne spp.) infecting cucumber plants: in vivo and in vitro |
| title_full_unstemmed | Comparative biological activity of abamectin formulations on root-knot nematodes (Meloidogyne spp.) infecting cucumber plants: in vivo and in vitro |
| title_short | Comparative biological activity of abamectin formulations on root-knot nematodes (Meloidogyne spp.) infecting cucumber plants: in vivo and in vitro |
| title_sort | comparative biological activity of abamectin formulations on root knot nematodes meloidogyne spp infecting cucumber plants in vivo and in vitro |
| url | https://doi.org/10.1038/s41598-023-39324-x |
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