Using High Resolution Melting Method for Detecting Nucleotide Polymorphisms of STK11 Gene Germline in Patients with Digestive System Cancers
Background and Objective: High resolution melting (HRM) analysis is a technique to measure decrease of fluoresce, caused by discharge of the dye, throughout DNA thermal melting gradient process. STK11 gene encodes one of cellular serine-threonine kinase proteins that regulates cellular polarity. It...
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| Format: | Article |
| Language: | English |
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West Asia Organization for Cancer Prevention
2016-06-01
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| Series: | Asian Pacific Journal of Cancer Biology |
| Subjects: | |
| Online Access: | http://www.waocp.com/journal/index.php/apjcb/article/view/197 |
| _version_ | 1797341174455861248 |
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| author | Seyed Jamal Hosseini Ali Nazemi Mehrdad Hashemi Mirsaed Miri Nargesi Sharashout Sharirfi Farhad Shaveisi Zadeh Ali Asghar Karaminia Abolfazl Movafagh |
| author_facet | Seyed Jamal Hosseini Ali Nazemi Mehrdad Hashemi Mirsaed Miri Nargesi Sharashout Sharirfi Farhad Shaveisi Zadeh Ali Asghar Karaminia Abolfazl Movafagh |
| author_sort | Seyed Jamal Hosseini |
| collection | DOAJ |
| description | Background and Objective: High resolution melting (HRM) analysis is a technique to measure decrease of fluoresce, caused by discharge of the dye, throughout DNA thermal melting gradient process. STK11 gene encodes one of cellular serine-threonine kinase proteins that regulates cellular polarity. It also acts as tumor suppressor protein. Mutations of germline in the gene is coincident with Peutz-Jeghers syndrome and potential of developing a variety of neoplasia.
Methodology: In an experimental study, genome DNA of 56 patients with digestive system cancer was extracted. Afterward, nucleotide changes over the gene were examined using Real-time PCR and High resolution melting (HRM).
Findings: Nucleotide screening using HRM technique revealed two types of SNP in introns 6 & 7 in 10 patients. Four patients showed homozygous C/T nucleotide changes [cluster id/dsSNP/rs9282860] in intron 6 and six patients showed heterozygous C/G nucleotide changes [cluster id/dsSNP/rs2075607] in intron 7. Comparison of HRM results with sequencing results indicated 100% conformity.
Conclusion: Although no mutation was observed in exon section of the gene, primary screening of STK11 gene to diagnose unknown nucleotide changes of germline and somatic in patients with neoplasia using HRM was feasible, easy, and cost effective. |
| first_indexed | 2024-03-08T10:14:03Z |
| format | Article |
| id | doaj.art-d30cef25ee204ebb8b3db31e6aad5a6c |
| institution | Directory Open Access Journal |
| issn | 2538-4635 |
| language | English |
| last_indexed | 2024-03-08T10:14:03Z |
| publishDate | 2016-06-01 |
| publisher | West Asia Organization for Cancer Prevention |
| record_format | Article |
| series | Asian Pacific Journal of Cancer Biology |
| spelling | doaj.art-d30cef25ee204ebb8b3db31e6aad5a6c2024-01-29T05:38:38ZengWest Asia Organization for Cancer PreventionAsian Pacific Journal of Cancer Biology2538-46352016-06-0112475010.31557/apjcb.2016.1.2.47-50197Using High Resolution Melting Method for Detecting Nucleotide Polymorphisms of STK11 Gene Germline in Patients with Digestive System CancersSeyed Jamal Hosseini0Ali Nazemi1Mehrdad Hashemi2Mirsaed Miri Nargesi3Sharashout Sharirfi4Farhad Shaveisi Zadeh5Ali Asghar Karaminia6Abolfazl MovafaghDepartment of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran.Department of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran.Department of Molecular Genetics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.Department of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran.Department of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran.Department of Medical Genetics, Cancer Research Center, Shohada, Hospital, School of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.Cancer Research Center, Shohada, Hospital, School of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.Background and Objective: High resolution melting (HRM) analysis is a technique to measure decrease of fluoresce, caused by discharge of the dye, throughout DNA thermal melting gradient process. STK11 gene encodes one of cellular serine-threonine kinase proteins that regulates cellular polarity. It also acts as tumor suppressor protein. Mutations of germline in the gene is coincident with Peutz-Jeghers syndrome and potential of developing a variety of neoplasia. Methodology: In an experimental study, genome DNA of 56 patients with digestive system cancer was extracted. Afterward, nucleotide changes over the gene were examined using Real-time PCR and High resolution melting (HRM). Findings: Nucleotide screening using HRM technique revealed two types of SNP in introns 6 & 7 in 10 patients. Four patients showed homozygous C/T nucleotide changes [cluster id/dsSNP/rs9282860] in intron 6 and six patients showed heterozygous C/G nucleotide changes [cluster id/dsSNP/rs2075607] in intron 7. Comparison of HRM results with sequencing results indicated 100% conformity. Conclusion: Although no mutation was observed in exon section of the gene, primary screening of STK11 gene to diagnose unknown nucleotide changes of germline and somatic in patients with neoplasia using HRM was feasible, easy, and cost effective.http://www.waocp.com/journal/index.php/apjcb/article/view/197stk11 gene, mutation of germline, high resolution melting |
| spellingShingle | Seyed Jamal Hosseini Ali Nazemi Mehrdad Hashemi Mirsaed Miri Nargesi Sharashout Sharirfi Farhad Shaveisi Zadeh Ali Asghar Karaminia Abolfazl Movafagh Using High Resolution Melting Method for Detecting Nucleotide Polymorphisms of STK11 Gene Germline in Patients with Digestive System Cancers Asian Pacific Journal of Cancer Biology stk11 gene, mutation of germline, high resolution melting |
| title | Using High Resolution Melting Method for Detecting Nucleotide Polymorphisms of STK11 Gene Germline in Patients with Digestive System Cancers |
| title_full | Using High Resolution Melting Method for Detecting Nucleotide Polymorphisms of STK11 Gene Germline in Patients with Digestive System Cancers |
| title_fullStr | Using High Resolution Melting Method for Detecting Nucleotide Polymorphisms of STK11 Gene Germline in Patients with Digestive System Cancers |
| title_full_unstemmed | Using High Resolution Melting Method for Detecting Nucleotide Polymorphisms of STK11 Gene Germline in Patients with Digestive System Cancers |
| title_short | Using High Resolution Melting Method for Detecting Nucleotide Polymorphisms of STK11 Gene Germline in Patients with Digestive System Cancers |
| title_sort | using high resolution melting method for detecting nucleotide polymorphisms of stk11 gene germline in patients with digestive system cancers |
| topic | stk11 gene, mutation of germline, high resolution melting |
| url | http://www.waocp.com/journal/index.php/apjcb/article/view/197 |
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