Collagen constitutes about 12% in females and 17% in males of the total protein in mice
Abstract Collagen has been postulated to be the most abundant protein in our body, making up one-third of the total protein content in mammals. However, a direct assessment of the total collagen levels of an entire mammal to confirm this estimate is missing. Here we measured hydroxyproline levels as...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-31566-z |
| _version_ | 1797864660082360320 |
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| author | Katharina Tarnutzer Devanarayanan Siva Sankar Joern Dengjel Collin Y. Ewald |
| author_facet | Katharina Tarnutzer Devanarayanan Siva Sankar Joern Dengjel Collin Y. Ewald |
| author_sort | Katharina Tarnutzer |
| collection | DOAJ |
| description | Abstract Collagen has been postulated to be the most abundant protein in our body, making up one-third of the total protein content in mammals. However, a direct assessment of the total collagen levels of an entire mammal to confirm this estimate is missing. Here we measured hydroxyproline levels as a proxy for collagen content together with total protein levels of entire mice or of individual tissues. Collagen content normalized to the total protein is approximately 0.1% in the brain and liver, 1% in the heart and kidney, 4% in the muscle and lung, 6% in the colon, 20–40% in the skin, 25–35% in bones, and 40–50% in tendons of wild-type (CD1 and CB57BL/6) mice, consistent with previous reports. To our surprise, we find that collagen is approximately 12% in females and 17% in males of the total protein content of entire wild-type (CD1 and CB57BL/6) mice. Although collagen type I is the most abundant collagen, the most abundant proteins are albumin, hemoglobulin, histones, actin, serpina, and then collagen type I. Analyzing amino acid compositions of mice revealed glycine as the most abundant amino acid. Thus, we provide reference points for collagen, matrisome, protein, and amino acid composition of healthy wild-type mice. |
| first_indexed | 2024-04-09T22:55:29Z |
| format | Article |
| id | doaj.art-d30fe29a12b34a62ac52396303daf5a3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:55:29Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d30fe29a12b34a62ac52396303daf5a32023-03-22T11:17:55ZengNature PortfolioScientific Reports2045-23222023-03-0113111210.1038/s41598-023-31566-zCollagen constitutes about 12% in females and 17% in males of the total protein in miceKatharina Tarnutzer0Devanarayanan Siva Sankar1Joern Dengjel2Collin Y. Ewald3Department of Health Sciences and Technology, Institute of Translational Medicine, ETH ZürichDepartment of Biology, University of FribourgDepartment of Biology, University of FribourgDepartment of Health Sciences and Technology, Institute of Translational Medicine, ETH ZürichAbstract Collagen has been postulated to be the most abundant protein in our body, making up one-third of the total protein content in mammals. However, a direct assessment of the total collagen levels of an entire mammal to confirm this estimate is missing. Here we measured hydroxyproline levels as a proxy for collagen content together with total protein levels of entire mice or of individual tissues. Collagen content normalized to the total protein is approximately 0.1% in the brain and liver, 1% in the heart and kidney, 4% in the muscle and lung, 6% in the colon, 20–40% in the skin, 25–35% in bones, and 40–50% in tendons of wild-type (CD1 and CB57BL/6) mice, consistent with previous reports. To our surprise, we find that collagen is approximately 12% in females and 17% in males of the total protein content of entire wild-type (CD1 and CB57BL/6) mice. Although collagen type I is the most abundant collagen, the most abundant proteins are albumin, hemoglobulin, histones, actin, serpina, and then collagen type I. Analyzing amino acid compositions of mice revealed glycine as the most abundant amino acid. Thus, we provide reference points for collagen, matrisome, protein, and amino acid composition of healthy wild-type mice.https://doi.org/10.1038/s41598-023-31566-z |
| spellingShingle | Katharina Tarnutzer Devanarayanan Siva Sankar Joern Dengjel Collin Y. Ewald Collagen constitutes about 12% in females and 17% in males of the total protein in mice Scientific Reports |
| title | Collagen constitutes about 12% in females and 17% in males of the total protein in mice |
| title_full | Collagen constitutes about 12% in females and 17% in males of the total protein in mice |
| title_fullStr | Collagen constitutes about 12% in females and 17% in males of the total protein in mice |
| title_full_unstemmed | Collagen constitutes about 12% in females and 17% in males of the total protein in mice |
| title_short | Collagen constitutes about 12% in females and 17% in males of the total protein in mice |
| title_sort | collagen constitutes about 12 in females and 17 in males of the total protein in mice |
| url | https://doi.org/10.1038/s41598-023-31566-z |
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