Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments
The human quest for sustainable habitation of extraterrestrial environments necessitates a robust understanding of life’s adaptability to the unique conditions of spaceflight. This study provides a comprehensive proteomic dissection of the Arabidopsis plant’s responses to the spaceflight environment...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/19/14425 |
| _version_ | 1797575847500054528 |
|---|---|
| author | Gbolaga O. Olanrewaju Colin P. S. Kruse Sarah E. Wyatt |
| author_facet | Gbolaga O. Olanrewaju Colin P. S. Kruse Sarah E. Wyatt |
| author_sort | Gbolaga O. Olanrewaju |
| collection | DOAJ |
| description | The human quest for sustainable habitation of extraterrestrial environments necessitates a robust understanding of life’s adaptability to the unique conditions of spaceflight. This study provides a comprehensive proteomic dissection of the Arabidopsis plant’s responses to the spaceflight environment through a meta-analysis of proteomics data from four separate spaceflight experiments conducted on the International Space Station (ISS) in different hardware configurations. Raw proteomics LC/MS spectra were analyzed for differential expression in MaxQuant and Perseus software. The analysis of dissimilarities among the datasets reveals the multidimensional nature of plant proteomic responses to spaceflight, impacted by variables such as spaceflight hardware, seedling age, lighting conditions, and proteomic quantification techniques. By contrasting datasets that varied in light exposure, we elucidated proteins involved in photomorphogenesis and skotomorphogenesis in plant spaceflight responses. Additionally, with data from an onboard 1 <i>g</i> control experiment, we isolated proteins that specifically respond to the microgravity environment and those that respond to other spaceflight conditions. This study identified proteins and associated metabolic pathways that are consistently impacted across the datasets. Notably, these shared proteins were associated with critical metabolic functions, including carbon metabolism, glycolysis, gluconeogenesis, and amino acid biosynthesis, underscoring their potential significance in Arabidopsis’ spaceflight adaptation mechanisms and informing strategies for successful space farming. |
| first_indexed | 2024-03-10T21:44:06Z |
| format | Article |
| id | doaj.art-d3105345f66d44b6a50a166220ac0370 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T21:44:06Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d3105345f66d44b6a50a166220ac03702023-11-19T14:26:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124191442510.3390/ijms241914425Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight ExperimentsGbolaga O. Olanrewaju0Colin P. S. Kruse1Sarah E. Wyatt2Molecular and Cellular Biology Program, Ohio University, Athens, OH 45701, USALos Alamos National Laboratory, Bioscience Division, Los Alamos, NM 87545, USAMolecular and Cellular Biology Program, Ohio University, Athens, OH 45701, USAThe human quest for sustainable habitation of extraterrestrial environments necessitates a robust understanding of life’s adaptability to the unique conditions of spaceflight. This study provides a comprehensive proteomic dissection of the Arabidopsis plant’s responses to the spaceflight environment through a meta-analysis of proteomics data from four separate spaceflight experiments conducted on the International Space Station (ISS) in different hardware configurations. Raw proteomics LC/MS spectra were analyzed for differential expression in MaxQuant and Perseus software. The analysis of dissimilarities among the datasets reveals the multidimensional nature of plant proteomic responses to spaceflight, impacted by variables such as spaceflight hardware, seedling age, lighting conditions, and proteomic quantification techniques. By contrasting datasets that varied in light exposure, we elucidated proteins involved in photomorphogenesis and skotomorphogenesis in plant spaceflight responses. Additionally, with data from an onboard 1 <i>g</i> control experiment, we isolated proteins that specifically respond to the microgravity environment and those that respond to other spaceflight conditions. This study identified proteins and associated metabolic pathways that are consistently impacted across the datasets. Notably, these shared proteins were associated with critical metabolic functions, including carbon metabolism, glycolysis, gluconeogenesis, and amino acid biosynthesis, underscoring their potential significance in Arabidopsis’ spaceflight adaptation mechanisms and informing strategies for successful space farming.https://www.mdpi.com/1422-0067/24/19/14425spaceflightgravitropismArabidopsisproteomicsmeta-analysisTMT |
| spellingShingle | Gbolaga O. Olanrewaju Colin P. S. Kruse Sarah E. Wyatt Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments International Journal of Molecular Sciences spaceflight gravitropism Arabidopsis proteomics meta-analysis TMT |
| title | Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments |
| title_full | Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments |
| title_fullStr | Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments |
| title_full_unstemmed | Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments |
| title_short | Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments |
| title_sort | functional meta analysis of the proteomic responses of arabidopsis seedlings to the spaceflight environment reveals multi dimensional sources of variability across spaceflight experiments |
| topic | spaceflight gravitropism Arabidopsis proteomics meta-analysis TMT |
| url | https://www.mdpi.com/1422-0067/24/19/14425 |
| work_keys_str_mv | AT gbolagaoolanrewaju functionalmetaanalysisoftheproteomicresponsesofarabidopsisseedlingstothespaceflightenvironmentrevealsmultidimensionalsourcesofvariabilityacrossspaceflightexperiments AT colinpskruse functionalmetaanalysisoftheproteomicresponsesofarabidopsisseedlingstothespaceflightenvironmentrevealsmultidimensionalsourcesofvariabilityacrossspaceflightexperiments AT sarahewyatt functionalmetaanalysisoftheproteomicresponsesofarabidopsisseedlingstothespaceflightenvironmentrevealsmultidimensionalsourcesofvariabilityacrossspaceflightexperiments |