The Shank3 interaction partner ProSAPiP1 regulates postsynaptic SPAR levels and the maturation of dendritic spines in hippocampal neurons
The postsynaptic density or PSD is a submembranous compartment containing a wide array of proteins that contribute to both morphology and function of excitatory glutamatergic synapses. In this study, we have analyzed functional aspects of the Fezzin ProSAPiP1, an interaction partner of the well-know...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-05-01
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| Series: | Frontiers in Synaptic Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnsyn.2016.00013/full |
| _version_ | 1819212670556962816 |
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| author | Dominik eReim Tobias eWeis Sonja eHalbedl Andreas eGrabrucker Michael J Schmeisser Tobias Maria Boeckers |
| author_facet | Dominik eReim Tobias eWeis Sonja eHalbedl Andreas eGrabrucker Michael J Schmeisser Tobias Maria Boeckers |
| author_sort | Dominik eReim |
| collection | DOAJ |
| description | The postsynaptic density or PSD is a submembranous compartment containing a wide array of proteins that contribute to both morphology and function of excitatory glutamatergic synapses. In this study, we have analyzed functional aspects of the Fezzin ProSAPiP1, an interaction partner of the well-known PSD proteins Shank3 and SPAR. Using lentiviral-mediated overexpression and knockdown of ProSAPiP1, we found that this protein is dispensable for the formation of both pre- and postsynaptic specializations per se. We further show that ProSAPiP1 regulates SPAR levels at the PSD and the maturation of dendritic spines. In line with previous findings on the ProSAPiP1 homologue PSD-Zip70, we conclude that Fezzins essentially contribute to the maturation of excitatory spine synapses. |
| first_indexed | 2024-12-23T06:46:39Z |
| format | Article |
| id | doaj.art-d3120d84e3114922b3d19764019542e7 |
| institution | Directory Open Access Journal |
| issn | 1663-3563 |
| language | English |
| last_indexed | 2024-12-23T06:46:39Z |
| publishDate | 2016-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Synaptic Neuroscience |
| spelling | doaj.art-d3120d84e3114922b3d19764019542e72022-12-21T17:56:33ZengFrontiers Media S.A.Frontiers in Synaptic Neuroscience1663-35632016-05-01810.3389/fnsyn.2016.00013187952The Shank3 interaction partner ProSAPiP1 regulates postsynaptic SPAR levels and the maturation of dendritic spines in hippocampal neuronsDominik eReim0Tobias eWeis1Sonja eHalbedl2Andreas eGrabrucker3Michael J Schmeisser4Tobias Maria Boeckers5University of UlmUniversity of UlmUniversity of UlmUniversity of UlmUniversity of UlmUniversity of UlmThe postsynaptic density or PSD is a submembranous compartment containing a wide array of proteins that contribute to both morphology and function of excitatory glutamatergic synapses. In this study, we have analyzed functional aspects of the Fezzin ProSAPiP1, an interaction partner of the well-known PSD proteins Shank3 and SPAR. Using lentiviral-mediated overexpression and knockdown of ProSAPiP1, we found that this protein is dispensable for the formation of both pre- and postsynaptic specializations per se. We further show that ProSAPiP1 regulates SPAR levels at the PSD and the maturation of dendritic spines. In line with previous findings on the ProSAPiP1 homologue PSD-Zip70, we conclude that Fezzins essentially contribute to the maturation of excitatory spine synapses.http://journal.frontiersin.org/Journal/10.3389/fnsyn.2016.00013/fullsynapseShank3sparLZTS1LAPSER1LZTS2 |
| spellingShingle | Dominik eReim Tobias eWeis Sonja eHalbedl Andreas eGrabrucker Michael J Schmeisser Tobias Maria Boeckers The Shank3 interaction partner ProSAPiP1 regulates postsynaptic SPAR levels and the maturation of dendritic spines in hippocampal neurons Frontiers in Synaptic Neuroscience synapse Shank3 spar LZTS1 LAPSER1 LZTS2 |
| title | The Shank3 interaction partner ProSAPiP1 regulates postsynaptic SPAR levels and the maturation of dendritic spines in hippocampal neurons |
| title_full | The Shank3 interaction partner ProSAPiP1 regulates postsynaptic SPAR levels and the maturation of dendritic spines in hippocampal neurons |
| title_fullStr | The Shank3 interaction partner ProSAPiP1 regulates postsynaptic SPAR levels and the maturation of dendritic spines in hippocampal neurons |
| title_full_unstemmed | The Shank3 interaction partner ProSAPiP1 regulates postsynaptic SPAR levels and the maturation of dendritic spines in hippocampal neurons |
| title_short | The Shank3 interaction partner ProSAPiP1 regulates postsynaptic SPAR levels and the maturation of dendritic spines in hippocampal neurons |
| title_sort | shank3 interaction partner prosapip1 regulates postsynaptic spar levels and the maturation of dendritic spines in hippocampal neurons |
| topic | synapse Shank3 spar LZTS1 LAPSER1 LZTS2 |
| url | http://journal.frontiersin.org/Journal/10.3389/fnsyn.2016.00013/full |
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