Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule
Piscirickettsia salmonis is the etiological agent of piscirickettsiosis, the most prevalent disease in salmonid species in Chilean salmonids farms. Many bacteria produce N-acyl-homoserine lactones (AHLs) as a quorum-sensing signal molecule to regulate gene expression in a cell density-dependent mann...
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.755496/full |
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| author | Pamela Ruiz Pamela Ruiz Daniela Sepulveda José Miguel Vidal José Miguel Vidal Romina Romero David Contreras Javier Barros Carlos Carrasco Nathaly Ruiz-Tagle Alex Romero Alex Romero Homero Urrutia Homero Urrutia Cristian Oliver |
| author_facet | Pamela Ruiz Pamela Ruiz Daniela Sepulveda José Miguel Vidal José Miguel Vidal Romina Romero David Contreras Javier Barros Carlos Carrasco Nathaly Ruiz-Tagle Alex Romero Alex Romero Homero Urrutia Homero Urrutia Cristian Oliver |
| author_sort | Pamela Ruiz |
| collection | DOAJ |
| description | Piscirickettsia salmonis is the etiological agent of piscirickettsiosis, the most prevalent disease in salmonid species in Chilean salmonids farms. Many bacteria produce N-acyl-homoserine lactones (AHLs) as a quorum-sensing signal molecule to regulate gene expression in a cell density-dependent manner, and thus modulate physiological characteristics and several bacterial mechanisms. In this study, a fluorescent biosensor system method and gas chromatography-tandem mass spectrometry (GC/MS) were combined to detect AHLs produced by P. salmonis. These analyses revealed an emitted fluorescence signal when the biosensor P. putida EL106 (RPL4cep) was co-cultured with both, P. salmonis LF-89 type strain and an EM-90-like strain Ps007, respectively. Furthermore, the production of an AHL-type molecule was confirmed by GC/MS by both P. salmonis strains, which identified the presence of a N-acetyl-L-homoserine Lactone in the supernatant extract. However, It is suggested that an alternate pathway could synthesizes AHLs, which should be address in future experiments in order to elucidate this important bacterial process. To the best of our knowledge, the present report is the first to describe the type of AHLs produced by P. salmonis. |
| first_indexed | 2024-12-21T08:27:51Z |
| format | Article |
| id | doaj.art-d312c5eb12684c8299cdb56a83f1e6ca |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-21T08:27:51Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d312c5eb12684c8299cdb56a83f1e6ca2022-12-21T19:10:17ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-10-011110.3389/fcimb.2021.755496755496Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related MoleculePamela Ruiz0Pamela Ruiz1Daniela Sepulveda2José Miguel Vidal3José Miguel Vidal4Romina Romero5David Contreras6Javier Barros7Carlos Carrasco8Nathaly Ruiz-Tagle9Alex Romero10Alex Romero11Homero Urrutia12Homero Urrutia13Cristian Oliver14Laboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, ChileDepartamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Talcahuano, ChileLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, ChileLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, ChileDepartamento de Investigación y Desarrollo, Ecombio limitada, Concepción, ChileLaboratorio de Investigaciones Medioambientales de Zonas Áridas (LIMZA), Depto. Ingeniería Mecánica, Facultad de Ingeniería, Universidad de Tarapacá, Arica, ChileDepartamento de Química Analítica e Inorgánica, Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, ChileDepartamento de Investigación y Desarrollo, Micbiotech Spa, Concepción, ChileLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, ChileLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, ChileLaboratorio de Inmunología y Estrés de Organismos Acuáticos, Facultad de Ciencias Veterinarias, Instituto de Patología Animal, Valdivia, ChileInterdisciplinary Center for Aquaculture Research (INCAR), Concepción, ChileLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, ChileDepartamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Inmunología y Estrés de Organismos Acuáticos, Facultad de Ciencias Veterinarias, Instituto de Patología Animal, Valdivia, ChilePiscirickettsia salmonis is the etiological agent of piscirickettsiosis, the most prevalent disease in salmonid species in Chilean salmonids farms. Many bacteria produce N-acyl-homoserine lactones (AHLs) as a quorum-sensing signal molecule to regulate gene expression in a cell density-dependent manner, and thus modulate physiological characteristics and several bacterial mechanisms. In this study, a fluorescent biosensor system method and gas chromatography-tandem mass spectrometry (GC/MS) were combined to detect AHLs produced by P. salmonis. These analyses revealed an emitted fluorescence signal when the biosensor P. putida EL106 (RPL4cep) was co-cultured with both, P. salmonis LF-89 type strain and an EM-90-like strain Ps007, respectively. Furthermore, the production of an AHL-type molecule was confirmed by GC/MS by both P. salmonis strains, which identified the presence of a N-acetyl-L-homoserine Lactone in the supernatant extract. However, It is suggested that an alternate pathway could synthesizes AHLs, which should be address in future experiments in order to elucidate this important bacterial process. To the best of our knowledge, the present report is the first to describe the type of AHLs produced by P. salmonis.https://www.frontiersin.org/articles/10.3389/fcimb.2021.755496/fullPiscirickettsia salmonisSRSquorum sensing (QS)N-acetyl-L-homoserine lactoneAHL (N-acyl-homoserine lactone) |
| spellingShingle | Pamela Ruiz Pamela Ruiz Daniela Sepulveda José Miguel Vidal José Miguel Vidal Romina Romero David Contreras Javier Barros Carlos Carrasco Nathaly Ruiz-Tagle Alex Romero Alex Romero Homero Urrutia Homero Urrutia Cristian Oliver Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule Frontiers in Cellular and Infection Microbiology Piscirickettsia salmonis SRS quorum sensing (QS) N-acetyl-L-homoserine lactone AHL (N-acyl-homoserine lactone) |
| title | Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule |
| title_full | Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule |
| title_fullStr | Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule |
| title_full_unstemmed | Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule |
| title_short | Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule |
| title_sort | piscirickettsia salmonis produces a n acetyl l homoserine lactone as a bacterial quorum sensing system related molecule |
| topic | Piscirickettsia salmonis SRS quorum sensing (QS) N-acetyl-L-homoserine lactone AHL (N-acyl-homoserine lactone) |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.755496/full |
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