Genomic and Transcriptomic Differentiation of Independent Invasions of the Pacific Oyster Crassostrea gigas
Upon colonizing new habitats, invasive species face a series of new selection pressures as a result of changing abiotic conditions and novel biotic interactions with native species. These new selection pressures can be accommodated by different mechanisms that act on different levels and across diff...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-10-01
|
| Series: | Frontiers in Ecology and Evolution |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fevo.2020.567049/full |
| _version_ | 1818655266554511360 |
|---|---|
| author | K. Mathias Wegner Ana Lokmer Ana Lokmer Uwe John Uwe John |
| author_facet | K. Mathias Wegner Ana Lokmer Ana Lokmer Uwe John Uwe John |
| author_sort | K. Mathias Wegner |
| collection | DOAJ |
| description | Upon colonizing new habitats, invasive species face a series of new selection pressures as a result of changing abiotic conditions and novel biotic interactions with native species. These new selection pressures can be accommodated by different mechanisms that act on different levels and across different time scales: (1) By changing transcriptomic profiles, species can react by plasticity within individual physiological limitations. (2) Invasive populations can adapt by fixing beneficial genetic variants in response to the newly encountered selection pressures. Here, we compare the genomic and transcriptomic landscapes of two independent invasions of the Pacific Oyster (Crassostrea gigas) into the North Sea. In detail, we combine ddRAD sequencing on the genomic level with RNAseq on the transcriptomic level to reveal outlier loci (SNPs) indicative of adaptation, as well as transcriptomic profiles from a translocation experiment to show immediate physiological reactions between two populations characterizing the two independent invasions. Generally, we found low physical congruence between differentially regulated genes and outlier loci, indicating that different genes are involved on the different time scales. Functionally matching outlier loci and differentially expressed genes were however found for spliceosomal modification of mRNA and particularly for transposon activation, indicating that these variation creating processes might be connected across eco-physiological and evolutionary time scales. By contrasting and identifying functional congruence between population outlier loci and population specific transcriptomic profiles, we can thus reveal a glimpse at the traits and processes characterizing specific mechanisms involved in successful invasions. |
| first_indexed | 2024-12-17T03:06:58Z |
| format | Article |
| id | doaj.art-d31369b1246b45a0b21a72027f67c729 |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-12-17T03:06:58Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-d31369b1246b45a0b21a72027f67c7292022-12-21T22:05:55ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2020-10-01810.3389/fevo.2020.567049567049Genomic and Transcriptomic Differentiation of Independent Invasions of the Pacific Oyster Crassostrea gigasK. Mathias Wegner0Ana Lokmer1Ana Lokmer2Uwe John3Uwe John4Alfred Wegener Institut – Helmholtz-Zentrum für Polar- und Meeresforschung, Coastal Ecology Section, Bremerhaven, GermanyAlfred Wegener Institut – Helmholtz-Zentrum für Polar- und Meeresforschung, Coastal Ecology Section, Bremerhaven, GermanyLaboratoire d’Eco-anthropologie et Ethnobiologie, Muséum National d’Histoire Naturelle, Paris, FranceAlfred Wegener Institut – Helmholtz-Zentrum für Polar- und Meeresforschung, Ecological Chemistry Section, Bremerhaven, GermanyHelmholtz Institute for Functional Marine Biodiversity, Oldenburg, GermanyUpon colonizing new habitats, invasive species face a series of new selection pressures as a result of changing abiotic conditions and novel biotic interactions with native species. These new selection pressures can be accommodated by different mechanisms that act on different levels and across different time scales: (1) By changing transcriptomic profiles, species can react by plasticity within individual physiological limitations. (2) Invasive populations can adapt by fixing beneficial genetic variants in response to the newly encountered selection pressures. Here, we compare the genomic and transcriptomic landscapes of two independent invasions of the Pacific Oyster (Crassostrea gigas) into the North Sea. In detail, we combine ddRAD sequencing on the genomic level with RNAseq on the transcriptomic level to reveal outlier loci (SNPs) indicative of adaptation, as well as transcriptomic profiles from a translocation experiment to show immediate physiological reactions between two populations characterizing the two independent invasions. Generally, we found low physical congruence between differentially regulated genes and outlier loci, indicating that different genes are involved on the different time scales. Functionally matching outlier loci and differentially expressed genes were however found for spliceosomal modification of mRNA and particularly for transposon activation, indicating that these variation creating processes might be connected across eco-physiological and evolutionary time scales. By contrasting and identifying functional congruence between population outlier loci and population specific transcriptomic profiles, we can thus reveal a glimpse at the traits and processes characterizing specific mechanisms involved in successful invasions.https://www.frontiersin.org/articles/10.3389/fevo.2020.567049/fullbiological invasiongenomicsgenetic differentiationFST outlieradaptationgene expression |
| spellingShingle | K. Mathias Wegner Ana Lokmer Ana Lokmer Uwe John Uwe John Genomic and Transcriptomic Differentiation of Independent Invasions of the Pacific Oyster Crassostrea gigas Frontiers in Ecology and Evolution biological invasion genomics genetic differentiation FST outlier adaptation gene expression |
| title | Genomic and Transcriptomic Differentiation of Independent Invasions of the Pacific Oyster Crassostrea gigas |
| title_full | Genomic and Transcriptomic Differentiation of Independent Invasions of the Pacific Oyster Crassostrea gigas |
| title_fullStr | Genomic and Transcriptomic Differentiation of Independent Invasions of the Pacific Oyster Crassostrea gigas |
| title_full_unstemmed | Genomic and Transcriptomic Differentiation of Independent Invasions of the Pacific Oyster Crassostrea gigas |
| title_short | Genomic and Transcriptomic Differentiation of Independent Invasions of the Pacific Oyster Crassostrea gigas |
| title_sort | genomic and transcriptomic differentiation of independent invasions of the pacific oyster crassostrea gigas |
| topic | biological invasion genomics genetic differentiation FST outlier adaptation gene expression |
| url | https://www.frontiersin.org/articles/10.3389/fevo.2020.567049/full |
| work_keys_str_mv | AT kmathiaswegner genomicandtranscriptomicdifferentiationofindependentinvasionsofthepacificoystercrassostreagigas AT analokmer genomicandtranscriptomicdifferentiationofindependentinvasionsofthepacificoystercrassostreagigas AT analokmer genomicandtranscriptomicdifferentiationofindependentinvasionsofthepacificoystercrassostreagigas AT uwejohn genomicandtranscriptomicdifferentiationofindependentinvasionsofthepacificoystercrassostreagigas AT uwejohn genomicandtranscriptomicdifferentiationofindependentinvasionsofthepacificoystercrassostreagigas |