Cre-lox neurogenetics: 20 years of versatile applications in brain research and counting...
Defining and manipulating specific neurons in the brain have garnered enormous interest in recent years, because such an approach is now recognized as crucial for deepening our understanding of how the brain works. When I started out to explore the Cre-loxP recombination for brain research in early...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-02-01
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| Series: | Frontiers in Genetics |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2016.00019/full |
| _version_ | 1819075037176528896 |
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| author | Joe Z Tsien |
| author_facet | Joe Z Tsien |
| author_sort | Joe Z Tsien |
| collection | DOAJ |
| description | Defining and manipulating specific neurons in the brain have garnered enormous interest in recent years, because such an approach is now recognized as crucial for deepening our understanding of how the brain works. When I started out to explore the Cre-loxP recombination for brain research in early 1990s, it was written off as a dead-end project by a young fool. Yet over the past 20 years, Cre-lox recombination-mediated neurogenetics has emerged as one of the most powerful and versatile technology platforms for cell-specific gene knockouts, transgenic overexpression, Brainbow imaging, neural pathway tracing with retrovirus and CLARITY, chemical genetics, and optogenetics. Its popularity and greater utility in neuroscience research is also largely thanks to the NIH’s bold Blueprint for Neuroscience Research Initiative to launch several Cre-driver resource projects, as well as individual laboratories and private research organizations. With newly-discovered, genetically-encoded molecules that are capable of responding to sonar and magnetic stimulation, for sonogenetics or magnetogenetics, respectively, or detecting rapid voltage changes in neurons, Cre-lox neurogenetics will continue to aid brain research for years to come. |
| first_indexed | 2024-12-21T18:19:02Z |
| format | Article |
| id | doaj.art-d3139290ae024d0db4a07b65c0450ecf |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-21T18:19:02Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-d3139290ae024d0db4a07b65c0450ecf2022-12-21T18:54:35ZengFrontiers Media S.A.Frontiers in Genetics1664-80212016-02-01710.3389/fgene.2016.00019180879Cre-lox neurogenetics: 20 years of versatile applications in brain research and counting...Joe Z Tsien0Medical College of Georgia at Augusta UniversityDefining and manipulating specific neurons in the brain have garnered enormous interest in recent years, because such an approach is now recognized as crucial for deepening our understanding of how the brain works. When I started out to explore the Cre-loxP recombination for brain research in early 1990s, it was written off as a dead-end project by a young fool. Yet over the past 20 years, Cre-lox recombination-mediated neurogenetics has emerged as one of the most powerful and versatile technology platforms for cell-specific gene knockouts, transgenic overexpression, Brainbow imaging, neural pathway tracing with retrovirus and CLARITY, chemical genetics, and optogenetics. Its popularity and greater utility in neuroscience research is also largely thanks to the NIH’s bold Blueprint for Neuroscience Research Initiative to launch several Cre-driver resource projects, as well as individual laboratories and private research organizations. With newly-discovered, genetically-encoded molecules that are capable of responding to sonar and magnetic stimulation, for sonogenetics or magnetogenetics, respectively, or detecting rapid voltage changes in neurons, Cre-lox neurogenetics will continue to aid brain research for years to come.http://journal.frontiersin.org/Journal/10.3389/fgene.2016.00019/fullCognitionneural circuitslearning and memoryoptogeneticsmouse brainBrainbow |
| spellingShingle | Joe Z Tsien Cre-lox neurogenetics: 20 years of versatile applications in brain research and counting... Frontiers in Genetics Cognition neural circuits learning and memory optogenetics mouse brain Brainbow |
| title | Cre-lox neurogenetics: 20 years of versatile applications in brain research and counting... |
| title_full | Cre-lox neurogenetics: 20 years of versatile applications in brain research and counting... |
| title_fullStr | Cre-lox neurogenetics: 20 years of versatile applications in brain research and counting... |
| title_full_unstemmed | Cre-lox neurogenetics: 20 years of versatile applications in brain research and counting... |
| title_short | Cre-lox neurogenetics: 20 years of versatile applications in brain research and counting... |
| title_sort | cre lox neurogenetics 20 years of versatile applications in brain research and counting |
| topic | Cognition neural circuits learning and memory optogenetics mouse brain Brainbow |
| url | http://journal.frontiersin.org/Journal/10.3389/fgene.2016.00019/full |
| work_keys_str_mv | AT joeztsien creloxneurogenetics20yearsofversatileapplicationsinbrainresearchandcounting |