In vitro analysis of antibacterial activity against wound pathogens, potential for wound healing, and anti‐melanoma properties of biosynthesized zinc oxide nanoparticles
Abstract Staphylococcus aureus is the most common cause of wound infections. Infected wounds increase wound severity and have a slower rate of healing. Moreover, emergence of multiple‐drug resistant bacteria such as methicillin‐resistant S. aureus (MRSA) limited treatment options. This study was the...
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| Format: | Article |
| Language: | English |
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Wiley
2023-09-01
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| Series: | Micro & Nano Letters |
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| Online Access: | https://doi.org/10.1049/mna2.12177 |
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| author | Ananya Jindaruk Titiradsadakorn Jaithon Jiraroj T‐Thienprasert Nattanan Panjaworayan T‐Thienprasert |
| author_facet | Ananya Jindaruk Titiradsadakorn Jaithon Jiraroj T‐Thienprasert Nattanan Panjaworayan T‐Thienprasert |
| author_sort | Ananya Jindaruk |
| collection | DOAJ |
| description | Abstract Staphylococcus aureus is the most common cause of wound infections. Infected wounds increase wound severity and have a slower rate of healing. Moreover, emergence of multiple‐drug resistant bacteria such as methicillin‐resistant S. aureus (MRSA) limited treatment options. This study was therefore aimed to evaluate antibacterial activity against wound pathogen and wound‐healing properties of green synthesized ZnO nanoparticles derived from mangosteen peel crude extract (ZnO‐Gm). Moreover, their anti‐skin cancer activity was also investigated in vitro. As a result, the ZnO‐Gm particles significantly inhibited growth of S. aureus and MRSA with the IC50 values at 0.44 and 0.51 mg/mL, respectively. By performing quantitative reactive oxygen species (ROS) assay, the intracellular ROS in both treated S. aureus and MRSA with ZnO‐Gm was found to be significantly elevated. Furthermore, ZnO‐Gm exhibited cytotoxic effects via induction of apoptosis on the A375 melanoma cancer cell line, with an IC50 value of 8.91 µg/mL, while not affecting the normal cell line (Vero). In addition, 30 µg/mL of ZnO‐Gm could strongly promote wound healing of an epidermal keratinocyte cell line (HaCaT). Consequently, the findings of this study demonstrated that the green synthesized ZnO nanoparticles have potential as antibacterial agents, wound‐healing materials, and anti‐melanoma agents. |
| first_indexed | 2024-03-09T14:21:48Z |
| format | Article |
| id | doaj.art-d313c72ab97d43d6a0d2679e0bb0b9f0 |
| institution | Directory Open Access Journal |
| issn | 1750-0443 |
| language | English |
| last_indexed | 2024-03-09T14:21:48Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Micro & Nano Letters |
| spelling | doaj.art-d313c72ab97d43d6a0d2679e0bb0b9f02023-11-28T10:32:40ZengWileyMicro & Nano Letters1750-04432023-09-01189-12n/an/a10.1049/mna2.12177In vitro analysis of antibacterial activity against wound pathogens, potential for wound healing, and anti‐melanoma properties of biosynthesized zinc oxide nanoparticlesAnanya Jindaruk0Titiradsadakorn Jaithon1Jiraroj T‐Thienprasert2Nattanan Panjaworayan T‐Thienprasert3Department of Biochemistry Faculty of Science Kasetsart University Bangkok ThailandDepartment of Biochemistry Faculty of Science Kasetsart University Bangkok ThailandDepartment of Physics Faculty of Science Kasetsart University Bangkok ThailandDepartment of Biochemistry Faculty of Science Kasetsart University Bangkok ThailandAbstract Staphylococcus aureus is the most common cause of wound infections. Infected wounds increase wound severity and have a slower rate of healing. Moreover, emergence of multiple‐drug resistant bacteria such as methicillin‐resistant S. aureus (MRSA) limited treatment options. This study was therefore aimed to evaluate antibacterial activity against wound pathogen and wound‐healing properties of green synthesized ZnO nanoparticles derived from mangosteen peel crude extract (ZnO‐Gm). Moreover, their anti‐skin cancer activity was also investigated in vitro. As a result, the ZnO‐Gm particles significantly inhibited growth of S. aureus and MRSA with the IC50 values at 0.44 and 0.51 mg/mL, respectively. By performing quantitative reactive oxygen species (ROS) assay, the intracellular ROS in both treated S. aureus and MRSA with ZnO‐Gm was found to be significantly elevated. Furthermore, ZnO‐Gm exhibited cytotoxic effects via induction of apoptosis on the A375 melanoma cancer cell line, with an IC50 value of 8.91 µg/mL, while not affecting the normal cell line (Vero). In addition, 30 µg/mL of ZnO‐Gm could strongly promote wound healing of an epidermal keratinocyte cell line (HaCaT). Consequently, the findings of this study demonstrated that the green synthesized ZnO nanoparticles have potential as antibacterial agents, wound‐healing materials, and anti‐melanoma agents.https://doi.org/10.1049/mna2.12177antibacterial activitybiological techniquesbiotechnologycancernanobiotechnologynanomedicine |
| spellingShingle | Ananya Jindaruk Titiradsadakorn Jaithon Jiraroj T‐Thienprasert Nattanan Panjaworayan T‐Thienprasert In vitro analysis of antibacterial activity against wound pathogens, potential for wound healing, and anti‐melanoma properties of biosynthesized zinc oxide nanoparticles Micro & Nano Letters antibacterial activity biological techniques biotechnology cancer nanobiotechnology nanomedicine |
| title | In vitro analysis of antibacterial activity against wound pathogens, potential for wound healing, and anti‐melanoma properties of biosynthesized zinc oxide nanoparticles |
| title_full | In vitro analysis of antibacterial activity against wound pathogens, potential for wound healing, and anti‐melanoma properties of biosynthesized zinc oxide nanoparticles |
| title_fullStr | In vitro analysis of antibacterial activity against wound pathogens, potential for wound healing, and anti‐melanoma properties of biosynthesized zinc oxide nanoparticles |
| title_full_unstemmed | In vitro analysis of antibacterial activity against wound pathogens, potential for wound healing, and anti‐melanoma properties of biosynthesized zinc oxide nanoparticles |
| title_short | In vitro analysis of antibacterial activity against wound pathogens, potential for wound healing, and anti‐melanoma properties of biosynthesized zinc oxide nanoparticles |
| title_sort | in vitro analysis of antibacterial activity against wound pathogens potential for wound healing and anti melanoma properties of biosynthesized zinc oxide nanoparticles |
| topic | antibacterial activity biological techniques biotechnology cancer nanobiotechnology nanomedicine |
| url | https://doi.org/10.1049/mna2.12177 |
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