High YKL-40 serum levels and its expression in the muscle tissues of patients with antisynthetase syndrome
Abstract Background The protein chitinase-3-like-1 (YKL-40) is rarely analyzed in patients with myositis. Therefore, we aimed to evaluate YKL-40 serum levels; correlate them with laboratory and clinical parameters, disease status, and treatment schemes; and analyze the YKL-40 expression in the muscl...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | Advances in Rheumatology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42358-021-00199-z |
| _version_ | 1818888650643996672 |
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| author | Renata Casseb de Souza Carboni Gustavo Luiz Behrens Pinto Samuel Katsuyuki Shinjo |
| author_facet | Renata Casseb de Souza Carboni Gustavo Luiz Behrens Pinto Samuel Katsuyuki Shinjo |
| author_sort | Renata Casseb de Souza Carboni |
| collection | DOAJ |
| description | Abstract Background The protein chitinase-3-like-1 (YKL-40) is rarely analyzed in patients with myositis. Therefore, we aimed to evaluate YKL-40 serum levels; correlate them with laboratory and clinical parameters, disease status, and treatment schemes; and analyze the YKL-40 expression in the muscle tissues of patients with antisynthetase syndrome (ASSD). Methods This cross-sectional single-center study included 64 adult patients with ASSD who were age-, gender-, and ethnicity-matched to 64 healthy control individuals. Their YKL-40 serum levels were analyzed using the Enzyme-Linked Immunosorbent Assay (ELISA) kit method, while YKL-40 expression in muscle tissues was analyzed using an immunohistochemical technique. Disease status was assessed using the International Myositis Assessment and Clinical Studies Group (IMACS) set scores. Results The patients’ mean age was 44.8 ± 11.8 years, and median disease duration was 1.5 (0.0–4.0) years. These patients were predominantly female (82.8%) and Caucasian (73.4%). Most patients had stable disease. The median YKL-40 serum level was significantly higher in patients with ASSD when compared to the healthy individuals: 538.4 (363.4–853.1) pg/mL versus 270.0 (201.8–451.9) pg/mL, respectively; P < 0.001. However, YKL-40 serum levels did not correlate with any clinical, laboratory, disease status, or therapeutic parameters (P > 0.050), except tumor necrosis factor alpha (TNF-α) serum levels (Spearman’s correlation, rho = 0.382; P = 0.007). YKL-40 was highly expressed by inflammatory cells found in muscle biopsy specimens. Conclusions High YKL-40 serum levels were observed in patients with ASSD and correlated positively with TNF-α serum levels. Moreover, YKL-40 was expressed by the inflammatory cells of the muscle tissue. |
| first_indexed | 2024-12-19T16:56:30Z |
| format | Article |
| id | doaj.art-d314e0fca0f749b58487d21ae9e90dba |
| institution | Directory Open Access Journal |
| issn | 2523-3106 |
| language | English |
| last_indexed | 2024-12-19T16:56:30Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Advances in Rheumatology |
| spelling | doaj.art-d314e0fca0f749b58487d21ae9e90dba2022-12-21T20:13:25ZengBMCAdvances in Rheumatology2523-31062021-07-0161111010.1186/s42358-021-00199-zHigh YKL-40 serum levels and its expression in the muscle tissues of patients with antisynthetase syndromeRenata Casseb de Souza Carboni0Gustavo Luiz Behrens Pinto1Samuel Katsuyuki Shinjo2Division of Rheumatology, Faculdade de Medicina FMUSP, Universidade de Sao PauloDivision of Rheumatology, Faculdade de Medicina FMUSP, Universidade de Sao PauloDivision of Rheumatology, Faculdade de Medicina FMUSP, Universidade de Sao PauloAbstract Background The protein chitinase-3-like-1 (YKL-40) is rarely analyzed in patients with myositis. Therefore, we aimed to evaluate YKL-40 serum levels; correlate them with laboratory and clinical parameters, disease status, and treatment schemes; and analyze the YKL-40 expression in the muscle tissues of patients with antisynthetase syndrome (ASSD). Methods This cross-sectional single-center study included 64 adult patients with ASSD who were age-, gender-, and ethnicity-matched to 64 healthy control individuals. Their YKL-40 serum levels were analyzed using the Enzyme-Linked Immunosorbent Assay (ELISA) kit method, while YKL-40 expression in muscle tissues was analyzed using an immunohistochemical technique. Disease status was assessed using the International Myositis Assessment and Clinical Studies Group (IMACS) set scores. Results The patients’ mean age was 44.8 ± 11.8 years, and median disease duration was 1.5 (0.0–4.0) years. These patients were predominantly female (82.8%) and Caucasian (73.4%). Most patients had stable disease. The median YKL-40 serum level was significantly higher in patients with ASSD when compared to the healthy individuals: 538.4 (363.4–853.1) pg/mL versus 270.0 (201.8–451.9) pg/mL, respectively; P < 0.001. However, YKL-40 serum levels did not correlate with any clinical, laboratory, disease status, or therapeutic parameters (P > 0.050), except tumor necrosis factor alpha (TNF-α) serum levels (Spearman’s correlation, rho = 0.382; P = 0.007). YKL-40 was highly expressed by inflammatory cells found in muscle biopsy specimens. Conclusions High YKL-40 serum levels were observed in patients with ASSD and correlated positively with TNF-α serum levels. Moreover, YKL-40 was expressed by the inflammatory cells of the muscle tissue.https://doi.org/10.1186/s42358-021-00199-zAntisynthetase syndromeChitinaseInflammatory myopathyMuscle biopsyMyositis |
| spellingShingle | Renata Casseb de Souza Carboni Gustavo Luiz Behrens Pinto Samuel Katsuyuki Shinjo High YKL-40 serum levels and its expression in the muscle tissues of patients with antisynthetase syndrome Advances in Rheumatology Antisynthetase syndrome Chitinase Inflammatory myopathy Muscle biopsy Myositis |
| title | High YKL-40 serum levels and its expression in the muscle tissues of patients with antisynthetase syndrome |
| title_full | High YKL-40 serum levels and its expression in the muscle tissues of patients with antisynthetase syndrome |
| title_fullStr | High YKL-40 serum levels and its expression in the muscle tissues of patients with antisynthetase syndrome |
| title_full_unstemmed | High YKL-40 serum levels and its expression in the muscle tissues of patients with antisynthetase syndrome |
| title_short | High YKL-40 serum levels and its expression in the muscle tissues of patients with antisynthetase syndrome |
| title_sort | high ykl 40 serum levels and its expression in the muscle tissues of patients with antisynthetase syndrome |
| topic | Antisynthetase syndrome Chitinase Inflammatory myopathy Muscle biopsy Myositis |
| url | https://doi.org/10.1186/s42358-021-00199-z |
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