Anti-Adhesion and Antibiofilm Activity of <i>Eruca sativa</i> Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach
Bacterial cells have the ability to form biofilm onto the surfaces of food matrixes and on food processing equipment, leading to a source of food contamination posing serious health implications. Therefore, our study aimed to determine the effect of <i>Eruca sativa</i> Miller <i>(E...
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MDPI AG
2022-02-01
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| Online Access: | https://www.mdpi.com/2223-7747/11/5/610 |
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| author | Amir Mahgoub Awadelkareem Eyad Al-Shammari AbdElmoneim O. Elkhalifa Mohd Adnan Arif Jamal Siddiqui Danish Mahmood Z. R. Azaz Ahmad Azad Mitesh Patel Khalid Mehmood Corina Danciu Syed Amir Ashraf |
| author_facet | Amir Mahgoub Awadelkareem Eyad Al-Shammari AbdElmoneim O. Elkhalifa Mohd Adnan Arif Jamal Siddiqui Danish Mahmood Z. R. Azaz Ahmad Azad Mitesh Patel Khalid Mehmood Corina Danciu Syed Amir Ashraf |
| author_sort | Amir Mahgoub Awadelkareem |
| collection | DOAJ |
| description | Bacterial cells have the ability to form biofilm onto the surfaces of food matrixes and on food processing equipment, leading to a source of food contamination posing serious health implications. Therefore, our study aimed to determine the effect of <i>Eruca sativa</i> Miller <i>(E. sativa)</i> crude extract against biofilms of food-borne bacteria along with in silico approaches to investigate adhesion proteins responsible for biofilm activity against the identified phytochemicals. The antibacterial potential of crude extract was evaluated using agar well diffusion technique and combinations of light and scanning electron microscopy to assess the efficacy of crude extract against the developed biofilms. Our results showed that crude extract of <i>E. sativa</i> was active against all tested food-borne bacteria, exhibiting a rapid kinetics of killing bacteria in a time-dependent manner. MIC and MBC values of <i>E. sativa</i> crude extract were found to be ranging from 125 to 500 µg/mL and 250 to 1000 µg/mL respectively. Furthermore, inhibition of developed biofilm by <i>E sativa</i> was found to be ranging from 58.68% to 73.45% for all the tested strains. The crude extract also reduced the viability of bacterial cells within biofilms and amount of EPS (ranging 59.73–82.77%) in the biofilm matrix. Additionally, the microscopic images also revealed significant disruption in the structure of biofilms. A molecular docking analysis of <i>E. sativa</i> phytochemicals showed interaction with active site of adhesion proteins <i>Sortase A</i>, <i>EspA</i>, <i>OprD</i>, and <i>type IV b pilin</i> of <i>S. aureus</i>, <i>E. coli</i>, <i>P. aeruginosa</i>, and <i>S. enterica ser.</i> typhi, respectively. Thus, our findings represent the first demonstration of <i>E. sativa</i> crude extract’s bioactivity and potency against food-borne bacteria in their planktonic forms, as well as against the developed biofilms. Therefore, a possible mechanistic approach for inhibition of biofilm via targeting adhesion proteins can be explored further to target biofilm producing food-borne bacterial pathogens. |
| first_indexed | 2024-03-09T20:26:35Z |
| format | Article |
| id | doaj.art-d31714507c87401880dfc74057e78405 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T20:26:35Z |
| publishDate | 2022-02-01 |
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| spelling | doaj.art-d31714507c87401880dfc74057e784052023-11-23T23:35:28ZengMDPI AGPlants2223-77472022-02-0111561010.3390/plants11050610Anti-Adhesion and Antibiofilm Activity of <i>Eruca sativa</i> Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico ApproachAmir Mahgoub Awadelkareem0Eyad Al-Shammari1AbdElmoneim O. Elkhalifa2Mohd Adnan3Arif Jamal Siddiqui4Danish Mahmood5Z. R. Azaz Ahmad Azad6Mitesh Patel7Khalid Mehmood8Corina Danciu9Syed Amir Ashraf10Department of Clinical Nutrition, College of Applied Medical Sciences, University of Hail, P.O. Box 2440, Hail 34464, Saudi ArabiaDepartment of Clinical Nutrition, College of Applied Medical Sciences, University of Hail, P.O. Box 2440, Hail 34464, Saudi ArabiaDepartment of Clinical Nutrition, College of Applied Medical Sciences, University of Hail, P.O. Box 2440, Hail 34464, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 34464, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 34464, Saudi ArabiaDepartment of Pharmacology and Toxicology, Unaizah College of Pharmacy, Qassim University, P.O. Box 6688, Qassim 51452, Saudi ArabiaDepartment of Post-Harvest Engineering and Technology, Aligarh Muslim University, Aligarh 202002, IndiaBapalal Vaidya Botanical Research Centre, Department of Biosciences, Veer Narmad South Gujarat University, Surat 395007, IndiaDepartment of Pharmaceutics, College of Pharmacy, University of Hail, P.O. Box 2440, Hail 34464, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaDepartment of Clinical Nutrition, College of Applied Medical Sciences, University of Hail, P.O. Box 2440, Hail 34464, Saudi ArabiaBacterial cells have the ability to form biofilm onto the surfaces of food matrixes and on food processing equipment, leading to a source of food contamination posing serious health implications. Therefore, our study aimed to determine the effect of <i>Eruca sativa</i> Miller <i>(E. sativa)</i> crude extract against biofilms of food-borne bacteria along with in silico approaches to investigate adhesion proteins responsible for biofilm activity against the identified phytochemicals. The antibacterial potential of crude extract was evaluated using agar well diffusion technique and combinations of light and scanning electron microscopy to assess the efficacy of crude extract against the developed biofilms. Our results showed that crude extract of <i>E. sativa</i> was active against all tested food-borne bacteria, exhibiting a rapid kinetics of killing bacteria in a time-dependent manner. MIC and MBC values of <i>E. sativa</i> crude extract were found to be ranging from 125 to 500 µg/mL and 250 to 1000 µg/mL respectively. Furthermore, inhibition of developed biofilm by <i>E sativa</i> was found to be ranging from 58.68% to 73.45% for all the tested strains. The crude extract also reduced the viability of bacterial cells within biofilms and amount of EPS (ranging 59.73–82.77%) in the biofilm matrix. Additionally, the microscopic images also revealed significant disruption in the structure of biofilms. A molecular docking analysis of <i>E. sativa</i> phytochemicals showed interaction with active site of adhesion proteins <i>Sortase A</i>, <i>EspA</i>, <i>OprD</i>, and <i>type IV b pilin</i> of <i>S. aureus</i>, <i>E. coli</i>, <i>P. aeruginosa</i>, and <i>S. enterica ser.</i> typhi, respectively. Thus, our findings represent the first demonstration of <i>E. sativa</i> crude extract’s bioactivity and potency against food-borne bacteria in their planktonic forms, as well as against the developed biofilms. Therefore, a possible mechanistic approach for inhibition of biofilm via targeting adhesion proteins can be explored further to target biofilm producing food-borne bacterial pathogens.https://www.mdpi.com/2223-7747/11/5/610<i>Eruca sativa</i> Millerfood-borne pathogensbiofilmsedible plantsadhesion proteinsextracellular polysaccharide |
| spellingShingle | Amir Mahgoub Awadelkareem Eyad Al-Shammari AbdElmoneim O. Elkhalifa Mohd Adnan Arif Jamal Siddiqui Danish Mahmood Z. R. Azaz Ahmad Azad Mitesh Patel Khalid Mehmood Corina Danciu Syed Amir Ashraf Anti-Adhesion and Antibiofilm Activity of <i>Eruca sativa</i> Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach Plants <i>Eruca sativa</i> Miller food-borne pathogens biofilms edible plants adhesion proteins extracellular polysaccharide |
| title | Anti-Adhesion and Antibiofilm Activity of <i>Eruca sativa</i> Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach |
| title_full | Anti-Adhesion and Antibiofilm Activity of <i>Eruca sativa</i> Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach |
| title_fullStr | Anti-Adhesion and Antibiofilm Activity of <i>Eruca sativa</i> Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach |
| title_full_unstemmed | Anti-Adhesion and Antibiofilm Activity of <i>Eruca sativa</i> Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach |
| title_short | Anti-Adhesion and Antibiofilm Activity of <i>Eruca sativa</i> Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach |
| title_sort | anti adhesion and antibiofilm activity of i eruca sativa i miller extract targeting cell adhesion proteins of food borne bacteria as a potential mechanism combined in vitro in silico approach |
| topic | <i>Eruca sativa</i> Miller food-borne pathogens biofilms edible plants adhesion proteins extracellular polysaccharide |
| url | https://www.mdpi.com/2223-7747/11/5/610 |
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