Influence of plasma collection tubes on N-glycome in human blood samples
Background and aims: Quantitative analysis of plasma N-glycome is a promising method for identifying disease biomarkers. This study aimed to investigate the impact of using blood collection tubes with different anticoagulants on plasma N-glycome. Materials and methods: We used a robust mass spectrom...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Practical Laboratory Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551724000295 |
| _version_ | 1797271352121491456 |
|---|---|
| author | Zejian Zhang Xiangyi Cui Nan Zhou Lisi Zhu Yuxiang Zhi Shuyang Zhang |
| author_facet | Zejian Zhang Xiangyi Cui Nan Zhou Lisi Zhu Yuxiang Zhi Shuyang Zhang |
| author_sort | Zejian Zhang |
| collection | DOAJ |
| description | Background and aims: Quantitative analysis of plasma N-glycome is a promising method for identifying disease biomarkers. This study aimed to investigate the impact of using blood collection tubes with different anticoagulants on plasma N-glycome. Materials and methods: We used a robust mass spectrometry method to profile plasma N-glycomes in two cohorts of healthy volunteers (cohort 1, n = 16; cohort 2, n = 53). The influence of three commonly used blood collection tubes on fully characterized N-glycomic profiles were explored. Results: Principal component analysis revealed distinct clustering of blood samples based on the collection tubes. Pairwise comparisons demonstrated significant differences between EDTA and heparin plasma in 55 out of 82 quantified N-glycan traits, and between EDTA and citrate plasma in 62 out of 82 traits. These differences encompassed various N-glycan features, including glycan type, sialylation, galactosylation, fucosylation, and bisection. Trends in N-glycan variations in citrate and heparin plasma were largely consistent compared to EDTA plasma. In correlation analysis (EDTA vs. heparin; EDTA vs. citrate), Pearson's correlation coefficients were consistently higher than 0.7 for the majority of N-glycan traits. Conclusion: Sample matrix variations impact plasma N-glycome measurements. Caution is crucial when comparing samples from different plasma collection tubes in glycomics projects. |
| first_indexed | 2024-03-07T14:01:29Z |
| format | Article |
| id | doaj.art-d31c6fc30bff425e97d5a7b56240fc1e |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-03-07T14:01:29Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-d31c6fc30bff425e97d5a7b56240fc1e2024-03-07T05:28:05ZengElsevierPractical Laboratory Medicine2352-55172024-03-0139e00383Influence of plasma collection tubes on N-glycome in human blood samplesZejian Zhang0Xiangyi Cui1Nan Zhou2Lisi Zhu3Yuxiang Zhi4Shuyang Zhang5State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuaifuyuan Wangfujing, Dongcheng District, Beijing, 100730, ChinaDepartment of Allergy & Clinical Immunology, National Clinical Research Center for Immunologic Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuaifuyuan Wangfujing, Dongcheng District, Beijing, 100730, ChinaDepartment of Allergy & Clinical Immunology, National Clinical Research Center for Immunologic Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuaifuyuan Wangfujing, Dongcheng District, Beijing, 100730, ChinaState Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuaifuyuan Wangfujing, Dongcheng District, Beijing, 100730, ChinaDepartment of Allergy & Clinical Immunology, National Clinical Research Center for Immunologic Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuaifuyuan Wangfujing, Dongcheng District, Beijing, 100730, China; Corresponding author.Department of Cardiology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuaifuyuan Wangfujing, Dongcheng District, Beijing, 100730, China; Corresponding author.Background and aims: Quantitative analysis of plasma N-glycome is a promising method for identifying disease biomarkers. This study aimed to investigate the impact of using blood collection tubes with different anticoagulants on plasma N-glycome. Materials and methods: We used a robust mass spectrometry method to profile plasma N-glycomes in two cohorts of healthy volunteers (cohort 1, n = 16; cohort 2, n = 53). The influence of three commonly used blood collection tubes on fully characterized N-glycomic profiles were explored. Results: Principal component analysis revealed distinct clustering of blood samples based on the collection tubes. Pairwise comparisons demonstrated significant differences between EDTA and heparin plasma in 55 out of 82 quantified N-glycan traits, and between EDTA and citrate plasma in 62 out of 82 traits. These differences encompassed various N-glycan features, including glycan type, sialylation, galactosylation, fucosylation, and bisection. Trends in N-glycan variations in citrate and heparin plasma were largely consistent compared to EDTA plasma. In correlation analysis (EDTA vs. heparin; EDTA vs. citrate), Pearson's correlation coefficients were consistently higher than 0.7 for the majority of N-glycan traits. Conclusion: Sample matrix variations impact plasma N-glycome measurements. Caution is crucial when comparing samples from different plasma collection tubes in glycomics projects.http://www.sciencedirect.com/science/article/pii/S2352551724000295N-glycomeCollection tubesPlasmaEDTAHeparinCitrate |
| spellingShingle | Zejian Zhang Xiangyi Cui Nan Zhou Lisi Zhu Yuxiang Zhi Shuyang Zhang Influence of plasma collection tubes on N-glycome in human blood samples Practical Laboratory Medicine N-glycome Collection tubes Plasma EDTA Heparin Citrate |
| title | Influence of plasma collection tubes on N-glycome in human blood samples |
| title_full | Influence of plasma collection tubes on N-glycome in human blood samples |
| title_fullStr | Influence of plasma collection tubes on N-glycome in human blood samples |
| title_full_unstemmed | Influence of plasma collection tubes on N-glycome in human blood samples |
| title_short | Influence of plasma collection tubes on N-glycome in human blood samples |
| title_sort | influence of plasma collection tubes on n glycome in human blood samples |
| topic | N-glycome Collection tubes Plasma EDTA Heparin Citrate |
| url | http://www.sciencedirect.com/science/article/pii/S2352551724000295 |
| work_keys_str_mv | AT zejianzhang influenceofplasmacollectiontubesonnglycomeinhumanbloodsamples AT xiangyicui influenceofplasmacollectiontubesonnglycomeinhumanbloodsamples AT nanzhou influenceofplasmacollectiontubesonnglycomeinhumanbloodsamples AT lisizhu influenceofplasmacollectiontubesonnglycomeinhumanbloodsamples AT yuxiangzhi influenceofplasmacollectiontubesonnglycomeinhumanbloodsamples AT shuyangzhang influenceofplasmacollectiontubesonnglycomeinhumanbloodsamples |