Presence and importance of oxidative stress parameters in malignant mammary gland tumors in dogs
This study aimed to evaluate the presence of oxidative stress based on lipid peroxidation and the DNA damage markers malondialdehyde (MDA) and 8-hydroxy-2"-deoxyguanosine (8-OHdG) in canine mammary gland carcinomas using immunohistochemistry techniques. A total of ten malignant and six normal c...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2021-07-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2824 |
| _version_ | 1797800735646154752 |
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| author | Emin KARAKURT Mushap KURU Serpil DAĞ Enver BEYTUT Hasan ORAL Hilmi NUHOĞLU Ayfer YILDIZ |
| author_facet | Emin KARAKURT Mushap KURU Serpil DAĞ Enver BEYTUT Hasan ORAL Hilmi NUHOĞLU Ayfer YILDIZ |
| author_sort | Emin KARAKURT |
| collection | DOAJ |
| description | This study aimed to evaluate the presence of oxidative stress based on lipid peroxidation and the DNA damage markers malondialdehyde
(MDA) and 8-hydroxy-2"-deoxyguanosine (8-OHdG) in canine mammary gland carcinomas using immunohistochemistry techniques. A total
of ten malignant and six normal canine mammary tissue samples were evaluated. The specimens were fixed in 10% buff ered formaldehyde
solution, processed routinely, embedded in paraffin wax, sectioned at 5 μm, stained with hematoxylin and eosin, examined under a light
microscope, and photographed to detect histopathological changes. For immunohistochemistry, the avidin-biotin-peroxidase method was
performed. All canine mammary gland tumors were immunopositive for MDA and 8-OHdG expression. There was a statistically significant
increase in MDA and 8-OHdG expressions in the tumor group compared to the control group. Based on this study data, in the context of
oxidative stress, it is proposed that lipid peroxidation and reactive oxygen species (ROS)-induced DNA damage are significantly associated
with canine mammary gland tumor development. In addition, antioxidants may be useful in the treatment of canine mammary gland tumors. |
| first_indexed | 2024-03-13T04:39:44Z |
| format | Article |
| id | doaj.art-d31fb31d33d34495b21702109517a1bf |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:39:44Z |
| publishDate | 2021-07-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-d31fb31d33d34495b21702109517a1bf2023-06-19T06:29:46ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512021-07-0127451752310.9775/kvfd.2021.259192824Presence and importance of oxidative stress parameters in malignant mammary gland tumors in dogsEmin KARAKURT0Mushap KURU1Serpil DAĞ2Enver BEYTUT3Hasan ORAL4Hilmi NUHOĞLU5Ayfer YILDIZ6Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEYDepartment of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEYDepartment of Pathology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEYDepartment of Pathology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEYDepartment of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEYDepartment of Pathology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEYDepartment of Pathology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEYThis study aimed to evaluate the presence of oxidative stress based on lipid peroxidation and the DNA damage markers malondialdehyde (MDA) and 8-hydroxy-2"-deoxyguanosine (8-OHdG) in canine mammary gland carcinomas using immunohistochemistry techniques. A total of ten malignant and six normal canine mammary tissue samples were evaluated. The specimens were fixed in 10% buff ered formaldehyde solution, processed routinely, embedded in paraffin wax, sectioned at 5 μm, stained with hematoxylin and eosin, examined under a light microscope, and photographed to detect histopathological changes. For immunohistochemistry, the avidin-biotin-peroxidase method was performed. All canine mammary gland tumors were immunopositive for MDA and 8-OHdG expression. There was a statistically significant increase in MDA and 8-OHdG expressions in the tumor group compared to the control group. Based on this study data, in the context of oxidative stress, it is proposed that lipid peroxidation and reactive oxygen species (ROS)-induced DNA damage are significantly associated with canine mammary gland tumor development. In addition, antioxidants may be useful in the treatment of canine mammary gland tumors.https://vetdergikafkas.org/pdf.php?id=2824caninecarcinomamammary glandoxidative stress |
| spellingShingle | Emin KARAKURT Mushap KURU Serpil DAĞ Enver BEYTUT Hasan ORAL Hilmi NUHOĞLU Ayfer YILDIZ Presence and importance of oxidative stress parameters in malignant mammary gland tumors in dogs Kafkas Universitesi Veteriner Fakültesi Dergisi canine carcinoma mammary gland oxidative stress |
| title | Presence and importance of oxidative stress parameters in malignant mammary gland tumors in dogs |
| title_full | Presence and importance of oxidative stress parameters in malignant mammary gland tumors in dogs |
| title_fullStr | Presence and importance of oxidative stress parameters in malignant mammary gland tumors in dogs |
| title_full_unstemmed | Presence and importance of oxidative stress parameters in malignant mammary gland tumors in dogs |
| title_short | Presence and importance of oxidative stress parameters in malignant mammary gland tumors in dogs |
| title_sort | presence and importance of oxidative stress parameters in malignant mammary gland tumors in dogs |
| topic | canine carcinoma mammary gland oxidative stress |
| url | https://vetdergikafkas.org/pdf.php?id=2824 |
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