Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in Korea
Cyprinids are popular species for aquaculture worldwide, with Asia being a significant contributor to their production. In Korea, common carp (Cyprinus carpio), koi carp (Cyprinus rubrofuscus), and goldfish (Carassius auratus) are cultivated domestically and imported for ornamental or human consumpt...
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| Format: | Article |
| Language: | English |
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The Korean Society of Fisheries and Aquatic Science
2023-07-01
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| Series: | Fisheries and Aquatic Sciences |
| Subjects: | |
| Online Access: | http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2023.e36 |
| _version_ | 1797770775978049536 |
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| author | Ye Jin Jeong Yu Gyeong Jeon Hee Ju Choi Eun Jin Baek Guk Hyun Kim Yun Jung Yang Min Jae Kim Joon Gyu Min Kwang Il Kim |
| author_facet | Ye Jin Jeong Yu Gyeong Jeon Hee Ju Choi Eun Jin Baek Guk Hyun Kim Yun Jung Yang Min Jae Kim Joon Gyu Min Kwang Il Kim |
| author_sort | Ye Jin Jeong |
| collection | DOAJ |
| description | Cyprinids are popular species for aquaculture worldwide, with Asia being a significant contributor to their production. In Korea, common carp (Cyprinus carpio), koi carp (Cyprinus rubrofuscus), and goldfish (Carassius auratus) are cultivated domestically and imported for ornamental or human consumption purposes. Among the viruses that infect cyprinids, cyprinid herpesvirus-2 (CyHV-2), koi herpesvirus (KHV, also known as cyprinid herpesvirus-3), and carp edema virus (CEV) are of particular concern as they cause substantial economic losses to the aquaculture industry. In this study, we investigated these viruses in both of domestic and imported cyprinids. Our results revealed that CyHV-2 was only detected in imported goldfish from Thailand. To further investigate the genetic characteristics of them, the marker A region was analyzed. Despite belonging to the same cluster with isolates from China, France, Poland, and Israel, CyHV-2 detected in this study showed distinct differences in their repetitive sequence sizes. Furthermore, two different forms of KHV/CEV coinfection were identified from domestic koi carp, both of which exhibited typical symptoms. Phylogenetic analysis showed that one KHV isolate (ScKc-2105-K) was of the Asian type and closely related to isolates from Japan, Indonesia, Belgium, Taiwan, and China. Two CEV isolates (ScKc-2105-CE and GhKc-2207-CE) belonged to the IIa type and showed high similarity with isolates from the USA, France, and Korea. Notably, koi carp injected with cultured KHV (ScKc-2105-K) showed 78.0% cumulative mortality within 14 days post-injection (dpi). Our findings support the importance of regular surveillance of viral diseases in cyprinids. |
| first_indexed | 2024-03-12T21:28:04Z |
| format | Article |
| id | doaj.art-d321928a2fd24fcaab3f6115afb277be |
| institution | Directory Open Access Journal |
| issn | 2234-1757 |
| language | English |
| last_indexed | 2024-03-12T21:28:04Z |
| publishDate | 2023-07-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj.art-d321928a2fd24fcaab3f6115afb277be2023-07-28T05:02:13ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572023-07-0126743744610.47853/FAS.2023.e36Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in KoreaYe Jin Jeong0Yu Gyeong Jeon1Hee Ju Choi2Eun Jin Baek3Guk Hyun Kim4Yun Jung Yang5Min Jae Kim6Joon Gyu Min7Kwang Il Kim8Department of Aquatic Life Medicine, Pukyong National University, Busan 48513, KoreaDepartment of Aquatic Life Medicine, Pukyong National University, Busan 48513, KoreaDepartment of Aquatic Life Medicine, Pukyong National University, Busan 48513, KoreaDepartment of Aquatic Life Medicine, Pukyong National University, Busan 48513, KoreaDepartment of Aquatic Life Medicine, Pukyong National University, Busan 48513, KoreaDepartment of Aquatic Life Medicine, Pukyong National University, Busan 48513, KoreaDepartment of Aquatic Life Medicine, Pukyong National University, Busan 48513, KoreaDepartment of Aquatic Life Medicine, Pukyong National University, Busan 48513, KoreaDepartment of Aquatic Life Medicine, Pukyong National University, Busan 48513, KoreaCyprinids are popular species for aquaculture worldwide, with Asia being a significant contributor to their production. In Korea, common carp (Cyprinus carpio), koi carp (Cyprinus rubrofuscus), and goldfish (Carassius auratus) are cultivated domestically and imported for ornamental or human consumption purposes. Among the viruses that infect cyprinids, cyprinid herpesvirus-2 (CyHV-2), koi herpesvirus (KHV, also known as cyprinid herpesvirus-3), and carp edema virus (CEV) are of particular concern as they cause substantial economic losses to the aquaculture industry. In this study, we investigated these viruses in both of domestic and imported cyprinids. Our results revealed that CyHV-2 was only detected in imported goldfish from Thailand. To further investigate the genetic characteristics of them, the marker A region was analyzed. Despite belonging to the same cluster with isolates from China, France, Poland, and Israel, CyHV-2 detected in this study showed distinct differences in their repetitive sequence sizes. Furthermore, two different forms of KHV/CEV coinfection were identified from domestic koi carp, both of which exhibited typical symptoms. Phylogenetic analysis showed that one KHV isolate (ScKc-2105-K) was of the Asian type and closely related to isolates from Japan, Indonesia, Belgium, Taiwan, and China. Two CEV isolates (ScKc-2105-CE and GhKc-2207-CE) belonged to the IIa type and showed high similarity with isolates from the USA, France, and Korea. Notably, koi carp injected with cultured KHV (ScKc-2105-K) showed 78.0% cumulative mortality within 14 days post-injection (dpi). Our findings support the importance of regular surveillance of viral diseases in cyprinids. http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2023.e36Koi herpesvirusCyprinid herpesvirus-2Carp edema virusCyprinidsTransmission |
| spellingShingle | Ye Jin Jeong Yu Gyeong Jeon Hee Ju Choi Eun Jin Baek Guk Hyun Kim Yun Jung Yang Min Jae Kim Joon Gyu Min Kwang Il Kim Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in Korea Fisheries and Aquatic Sciences Koi herpesvirus Cyprinid herpesvirus-2 Carp edema virus Cyprinids Transmission |
| title | Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in Korea |
| title_full | Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in Korea |
| title_fullStr | Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in Korea |
| title_full_unstemmed | Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in Korea |
| title_short | Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in Korea |
| title_sort | genetic characterization of alloherpesvirus cyprinid herpesvirus 2 and koi herpesvirus and poxvirus carp edema virus identified from domestic and imported cyprinids in korea |
| topic | Koi herpesvirus Cyprinid herpesvirus-2 Carp edema virus Cyprinids Transmission |
| url | http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2023.e36 |
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