Crude Anthrax Protective Antigen Enhances Immunity For Salmonella Typhimurium in Mice
Background:Non-host-adapted Salmonella serovar Typhimurium is a facultative intracellular bacterium, which invades and multiplies within mononuclear phagocytes in liver, spleen, lymph nodes and Peyer’s plaques. Salmonella infection is a crucial medical and veterinary problem globally. S. Typhimurium...
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| Format: | Article |
| Language: | English |
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College of Medicine University of Baghdad
2017-07-01
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| Series: | مجلة كلية الطب |
| Subjects: | |
| Online Access: | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/133 |
| _version_ | 1797332975813132288 |
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| author | Inam J. Lafta Huda H. Al abbody |
| author_facet | Inam J. Lafta Huda H. Al abbody |
| author_sort | Inam J. Lafta |
| collection | DOAJ |
| description | Background:Non-host-adapted Salmonella serovar Typhimurium is a facultative intracellular bacterium, which invades and multiplies within mononuclear phagocytes in liver, spleen, lymph nodes and Peyer’s plaques. Salmonella infection is a crucial medical and veterinary problem globally. S. Typhimurium causes various clinical symptoms, from asymptomatic infection to typhoid-like syndromes in infants or highly susceptible animals, for instance mice.
Objective: The present study was carried out to investigate the efficacy of anthrax protective antigen (PA)as a potent adjuvant mixed with killed Salmonella Typhimurium (S.T.) to enhance the immunization capacity of the last.
Materials and Methods: Two groups of mice were immunized with either the mixture of S.T. and PA or S.T. alone, in addition to a control group immunized with phosphate buffered saline. Skin test and ELISA test were performed to evaluate cell-mediated and humoral immunity, respectively.
Results: The mixture group showed the best results in cell-mediated immunity.Furthermore, ELISA reading 14 days from booster dose was higher in the mixture group than S.T. alone. The mixture revealed high Ab titres compared to control or S.T. cohort 53 days from the beginning of immunization.These results were verified by histopathological examination.
Conclusions: Our findings suggest anthrax PA as a suitable and robust adjuvant for inactivated Salmonella Typhimurium vaccine in mice. |
| first_indexed | 2024-03-08T07:57:08Z |
| format | Article |
| id | doaj.art-d321f7ac9d6b495da46a8a1a8b6c1b3c |
| institution | Directory Open Access Journal |
| issn | 0041-9419 2410-8057 |
| language | English |
| last_indexed | 2024-03-08T07:57:08Z |
| publishDate | 2017-07-01 |
| publisher | College of Medicine University of Baghdad |
| record_format | Article |
| series | مجلة كلية الطب |
| spelling | doaj.art-d321f7ac9d6b495da46a8a1a8b6c1b3c2024-02-02T13:30:24ZengCollege of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572017-07-0159210.32007/med.1936/jfacmedbagdad.v59i2.16Crude Anthrax Protective Antigen Enhances Immunity For Salmonella Typhimurium in MiceInam J. Lafta0Huda H. Al abbody1College of Veterinary Medicine, Zoonotic Diseases Unit, Baghdad UniversityIraqi National Cancer Research Center, Baghdad University.Background:Non-host-adapted Salmonella serovar Typhimurium is a facultative intracellular bacterium, which invades and multiplies within mononuclear phagocytes in liver, spleen, lymph nodes and Peyer’s plaques. Salmonella infection is a crucial medical and veterinary problem globally. S. Typhimurium causes various clinical symptoms, from asymptomatic infection to typhoid-like syndromes in infants or highly susceptible animals, for instance mice. Objective: The present study was carried out to investigate the efficacy of anthrax protective antigen (PA)as a potent adjuvant mixed with killed Salmonella Typhimurium (S.T.) to enhance the immunization capacity of the last. Materials and Methods: Two groups of mice were immunized with either the mixture of S.T. and PA or S.T. alone, in addition to a control group immunized with phosphate buffered saline. Skin test and ELISA test were performed to evaluate cell-mediated and humoral immunity, respectively. Results: The mixture group showed the best results in cell-mediated immunity.Furthermore, ELISA reading 14 days from booster dose was higher in the mixture group than S.T. alone. The mixture revealed high Ab titres compared to control or S.T. cohort 53 days from the beginning of immunization.These results were verified by histopathological examination. Conclusions: Our findings suggest anthrax PA as a suitable and robust adjuvant for inactivated Salmonella Typhimurium vaccine in mice.http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/133Salmonella Typhimurium , Antrax protective antigen , killed vaccine , skin test and ELISA. |
| spellingShingle | Inam J. Lafta Huda H. Al abbody Crude Anthrax Protective Antigen Enhances Immunity For Salmonella Typhimurium in Mice مجلة كلية الطب Salmonella Typhimurium , Antrax protective antigen , killed vaccine , skin test and ELISA. |
| title | Crude Anthrax Protective Antigen Enhances Immunity For Salmonella Typhimurium in Mice |
| title_full | Crude Anthrax Protective Antigen Enhances Immunity For Salmonella Typhimurium in Mice |
| title_fullStr | Crude Anthrax Protective Antigen Enhances Immunity For Salmonella Typhimurium in Mice |
| title_full_unstemmed | Crude Anthrax Protective Antigen Enhances Immunity For Salmonella Typhimurium in Mice |
| title_short | Crude Anthrax Protective Antigen Enhances Immunity For Salmonella Typhimurium in Mice |
| title_sort | crude anthrax protective antigen enhances immunity for salmonella typhimurium in mice |
| topic | Salmonella Typhimurium , Antrax protective antigen , killed vaccine , skin test and ELISA. |
| url | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/133 |
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