The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica
Our present study concerns the influence of the picosecond rise-time-pulsed electromagnetic field, and the impact of nanosecond pulsed pressure on the aggregation state of horseradish peroxidase (HRP) as a model enzyme. The influence of a 640 kV/m pulsed electromagnetic field with a pulse rise-time...
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MDPI AG
2021-12-01
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| author | Vadim S. Ziborov Tatyana O. Pleshakova Ivan D. Shumov Andrey F. Kozlov Anastasia A. Valueva Irina A. Ivanova Maria O. Ershova Dmitry I. Larionov Alexey N. Evdokimov Vadim Yu. Tatur Alexander I. Aleshko Konstantin Yu. Sakharov Alexander Yu. Dolgoborodov Vladimir E. Fortov Alexander I. Archakov Yuri D. Ivanov |
| author_facet | Vadim S. Ziborov Tatyana O. Pleshakova Ivan D. Shumov Andrey F. Kozlov Anastasia A. Valueva Irina A. Ivanova Maria O. Ershova Dmitry I. Larionov Alexey N. Evdokimov Vadim Yu. Tatur Alexander I. Aleshko Konstantin Yu. Sakharov Alexander Yu. Dolgoborodov Vladimir E. Fortov Alexander I. Archakov Yuri D. Ivanov |
| author_sort | Vadim S. Ziborov |
| collection | DOAJ |
| description | Our present study concerns the influence of the picosecond rise-time-pulsed electromagnetic field, and the impact of nanosecond pulsed pressure on the aggregation state of horseradish peroxidase (HRP) as a model enzyme. The influence of a 640 kV/m pulsed electromagnetic field with a pulse rise-time of ~200 ps on the activity and aggregation state of an enzyme is studied by the single-molecule atomic force microscopy (AFM) method. The influence of such a field is shown to lead to aggregation of the protein and to a decrease in its enzymatic activity. Moreover, the effect of a shock wave with a pressure front rise-time of 80 ns on the increase in the HRP aggregation is demonstrated. The results obtained herein can be of use in modeling the impact of electromagnetic and pressure pulses on enzymes and on whole living organisms. Our results are also important for taking into account the effect of pulsed fields on the body in the development of drugs, therapeutic procedures, and novel highly sensitive medical diagnosticums. |
| first_indexed | 2024-03-10T04:38:00Z |
| format | Article |
| id | doaj.art-d32205bff949499a9eeeaff61bc211c8 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T04:38:00Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-d32205bff949499a9eeeaff61bc211c82023-11-23T03:36:37ZengMDPI AGApplied Sciences2076-34172021-12-0111241167710.3390/app112411677The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto MicaVadim S. Ziborov0Tatyana O. Pleshakova1Ivan D. Shumov2Andrey F. Kozlov3Anastasia A. Valueva4Irina A. Ivanova5Maria O. Ershova6Dmitry I. Larionov7Alexey N. Evdokimov8Vadim Yu. Tatur9Alexander I. Aleshko10Konstantin Yu. Sakharov11Alexander Yu. Dolgoborodov12Vladimir E. Fortov13Alexander I. Archakov14Yuri D. Ivanov15Institute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, RussiaFoundation of Perspective Technologies and Novations, 115682 Moscow, RussiaFoundation of Perspective Technologies and Novations, 115682 Moscow, RussiaThe All-Russian Research Institute for Optical and Physical Measurements Federal State Unitary Enterprise (VNIIOFI) of the Federal Agency for Technical Regulation and Metrology of the Russian Federation, 119361 Moscow, RussiaThe All-Russian Research Institute for Optical and Physical Measurements Federal State Unitary Enterprise (VNIIOFI) of the Federal Agency for Technical Regulation and Metrology of the Russian Federation, 119361 Moscow, RussiaJoint Institute for High Temperatures of the Russian Academy of Sciences, 125412 Moscow, RussiaJoint Institute for High Temperatures of the Russian Academy of Sciences, 125412 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, RussiaOur present study concerns the influence of the picosecond rise-time-pulsed electromagnetic field, and the impact of nanosecond pulsed pressure on the aggregation state of horseradish peroxidase (HRP) as a model enzyme. The influence of a 640 kV/m pulsed electromagnetic field with a pulse rise-time of ~200 ps on the activity and aggregation state of an enzyme is studied by the single-molecule atomic force microscopy (AFM) method. The influence of such a field is shown to lead to aggregation of the protein and to a decrease in its enzymatic activity. Moreover, the effect of a shock wave with a pressure front rise-time of 80 ns on the increase in the HRP aggregation is demonstrated. The results obtained herein can be of use in modeling the impact of electromagnetic and pressure pulses on enzymes and on whole living organisms. Our results are also important for taking into account the effect of pulsed fields on the body in the development of drugs, therapeutic procedures, and novel highly sensitive medical diagnosticums.https://www.mdpi.com/2076-3417/11/24/11677horseradish peroxidaseatomic force microscopyprotein aggregationshock wavepulsed electromagnetic field |
| spellingShingle | Vadim S. Ziborov Tatyana O. Pleshakova Ivan D. Shumov Andrey F. Kozlov Anastasia A. Valueva Irina A. Ivanova Maria O. Ershova Dmitry I. Larionov Alexey N. Evdokimov Vadim Yu. Tatur Alexander I. Aleshko Konstantin Yu. Sakharov Alexander Yu. Dolgoborodov Vladimir E. Fortov Alexander I. Archakov Yuri D. Ivanov The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica Applied Sciences horseradish peroxidase atomic force microscopy protein aggregation shock wave pulsed electromagnetic field |
| title | The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica |
| title_full | The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica |
| title_fullStr | The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica |
| title_full_unstemmed | The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica |
| title_short | The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica |
| title_sort | impact of fast rise time electromagnetic field and pressure on the aggregation of peroxidase upon its adsorption onto mica |
| topic | horseradish peroxidase atomic force microscopy protein aggregation shock wave pulsed electromagnetic field |
| url | https://www.mdpi.com/2076-3417/11/24/11677 |
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