Copper Tolerance Mediated by FgAceA and FgCrpA in Fusarium graminearum
All organisms must secure essential trace elements (e.g., Cu) for survival and reproduction. However, excess trace element accumulation in cells is highly toxic. The maintenance of copper (Cu) homeostasis has been extensively studied in mammals, bacteria, and yeast but not in plant pathogens. In thi...
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| Format: | Article |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01392/full |
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| author | Xin Liu Xin Liu Xin Liu Yichen Jiang Yichen Jiang Dan He Xin Fang Xin Fang Jianhong Xu Jianhong Xu Yin-Won Lee Yin-Won Lee Nancy P. Keller Nancy P. Keller Jianrong Shi Jianrong Shi |
| author_facet | Xin Liu Xin Liu Xin Liu Yichen Jiang Yichen Jiang Dan He Xin Fang Xin Fang Jianhong Xu Jianhong Xu Yin-Won Lee Yin-Won Lee Nancy P. Keller Nancy P. Keller Jianrong Shi Jianrong Shi |
| author_sort | Xin Liu |
| collection | DOAJ |
| description | All organisms must secure essential trace elements (e.g., Cu) for survival and reproduction. However, excess trace element accumulation in cells is highly toxic. The maintenance of copper (Cu) homeostasis has been extensively studied in mammals, bacteria, and yeast but not in plant pathogens. In this study, we investigated the molecular mechanisms of copper tolerance in Fusarium graminearum, the important wheat head scab fungus. RNA-seq revealed induced expression of the P-type ATPase transporter FgCrpA and metallothionein (MT) FgCrdA after excess Cu treatment. Deletion of FgCrpA but not FgCrdA resulted in reduced tolerance to Cu toxicity. The “Cu fist” transcription factor FgAceA was involved in Cu detoxification through activation of FgCrpA. △FgAceA was more sensitive to copper toxicity than △FgCrpA and overexpression of FgCrpA restored copper tolerance in △FgAceA. FgAceA negatively regulated aurofusarin production and its biosynthetic gene expression. △FgCrpA and △FgAceA were reduced in virulence in flowering wheat heads and synthesized decreased amounts of the mycotoxin deoxynivalenol when challenged with excess Cu. Taken together, these results suggest that mediation of Cu tolerance in F. graminearum mainly relies on the Cu efflux pump and that FgAceA governs Cu detoxification through activation of FgCrpA. |
| first_indexed | 2024-12-11T13:33:14Z |
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| id | doaj.art-d324806b2e9e4acc8b28b3c68aa0e04d |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T13:33:14Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d324806b2e9e4acc8b28b3c68aa0e04d2022-12-22T01:05:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.01392548639Copper Tolerance Mediated by FgAceA and FgCrpA in Fusarium graminearumXin Liu0Xin Liu1Xin Liu2Yichen Jiang3Yichen Jiang4Dan He5Xin Fang6Xin Fang7Jianhong Xu8Jianhong Xu9Yin-Won Lee10Yin-Won Lee11Nancy P. Keller12Nancy P. Keller13Jianrong Shi14Jianrong Shi15Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, United StatesSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaCollege of Food Science, Tibet Agriculture and Animal Husbandry University, Nyingchi, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSchool of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, United StatesDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI, United StatesJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaAll organisms must secure essential trace elements (e.g., Cu) for survival and reproduction. However, excess trace element accumulation in cells is highly toxic. The maintenance of copper (Cu) homeostasis has been extensively studied in mammals, bacteria, and yeast but not in plant pathogens. In this study, we investigated the molecular mechanisms of copper tolerance in Fusarium graminearum, the important wheat head scab fungus. RNA-seq revealed induced expression of the P-type ATPase transporter FgCrpA and metallothionein (MT) FgCrdA after excess Cu treatment. Deletion of FgCrpA but not FgCrdA resulted in reduced tolerance to Cu toxicity. The “Cu fist” transcription factor FgAceA was involved in Cu detoxification through activation of FgCrpA. △FgAceA was more sensitive to copper toxicity than △FgCrpA and overexpression of FgCrpA restored copper tolerance in △FgAceA. FgAceA negatively regulated aurofusarin production and its biosynthetic gene expression. △FgCrpA and △FgAceA were reduced in virulence in flowering wheat heads and synthesized decreased amounts of the mycotoxin deoxynivalenol when challenged with excess Cu. Taken together, these results suggest that mediation of Cu tolerance in F. graminearum mainly relies on the Cu efflux pump and that FgAceA governs Cu detoxification through activation of FgCrpA.https://www.frontiersin.org/article/10.3389/fmicb.2020.01392/fullFusarium graminearumcopper toleranceFgAceAcopper transportersmetallothioneinvirulence |
| spellingShingle | Xin Liu Xin Liu Xin Liu Yichen Jiang Yichen Jiang Dan He Xin Fang Xin Fang Jianhong Xu Jianhong Xu Yin-Won Lee Yin-Won Lee Nancy P. Keller Nancy P. Keller Jianrong Shi Jianrong Shi Copper Tolerance Mediated by FgAceA and FgCrpA in Fusarium graminearum Frontiers in Microbiology Fusarium graminearum copper tolerance FgAceA copper transporters metallothionein virulence |
| title | Copper Tolerance Mediated by FgAceA and FgCrpA in Fusarium graminearum |
| title_full | Copper Tolerance Mediated by FgAceA and FgCrpA in Fusarium graminearum |
| title_fullStr | Copper Tolerance Mediated by FgAceA and FgCrpA in Fusarium graminearum |
| title_full_unstemmed | Copper Tolerance Mediated by FgAceA and FgCrpA in Fusarium graminearum |
| title_short | Copper Tolerance Mediated by FgAceA and FgCrpA in Fusarium graminearum |
| title_sort | copper tolerance mediated by fgacea and fgcrpa in fusarium graminearum |
| topic | Fusarium graminearum copper tolerance FgAceA copper transporters metallothionein virulence |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01392/full |
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